Construct: ORF TRCN0000471971
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009883.1_s317c1
- Derived from:
- ccsbBroadEn_05423
- DNA Barcode:
- ACTCCGCCGCGTCAATCGCGAACT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB37 (326624)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471971
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 326624 | RAB37 | RAB37, member RAS oncogene ... | NM_001006638.3 | 100% | 100% | |
| 2 | human | 326624 | RAB37 | RAB37, member RAS oncogene ... | NM_001163989.1 | 91.6% | 87.8% | (many diffs) |
| 3 | human | 326624 | RAB37 | RAB37, member RAS oncogene ... | NM_001330471.1 | 87.4% | 86.5% | (many diffs) |
| 4 | human | 326624 | RAB37 | RAB37, member RAS oncogene ... | NM_175738.5 | 86.3% | 79.6% | (many diffs) |
| 5 | human | 326624 | RAB37 | RAB37, member RAS oncogene ... | NM_001163990.2 | 83.4% | 83.4% | 92_93ins111 |
| 6 | mouse | 58222 | Rab37 | RAB37, member RAS oncogene ... | NM_021411.4 | 88.9% | 93.7% | (many diffs) |
| 7 | mouse | 58222 | Rab37 | RAB37, member RAS oncogene ... | XM_006533856.3 | 72.5% | 68.2% | (many diffs) |
| 8 | mouse | 58222 | Rab37 | RAB37, member RAS oncogene ... | XR_388502.3 | 25.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 735
- ORF length:
- 669
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gggcacgcca ggcgccgttg ccacccggga tggcgaggcc cccgagcgct 121 ccccgccctg cagtccgagc tacgacctca cgggcaaggt gatgcttctg ggagacacag 181 gcgtcggcaa aacatgtttc ctgatccaat tcaaagacgg ggccttcctg tccggaacct 241 tcatagccac cGTCGGCATA GACTTCAGGA ACAAGGTGGT GACTGTGGAT GGCGTGAGAG 301 TGAAGCTGCA GATCTGGGAC ACCGCTGGGC AGGAACGGTT CCGAAGCGTC ACCCATGCTT 361 ATTACAGAGA TGCTCAGGCC TTGCTTCTGC TGTATGACAT CACCAACAAA TCTTCTTTCG 421 ACAACATCAG GGCCTGGCTC ACTGAGATTC ATGAGTATGC CCAGAGGGAC GTGGTGATCA 481 TGCTGCTAGG CAACAAGGCG GATATGAGCA GCGAAAGAGT GATCCGTTCC GAAGACGGAG 541 AGACCTTGGC CAGGGAGTAC GGTGTTCCCT TCCTGGAGAC CAGCGCCAAG ACTGGCATGA 601 ATGTGGAGTT AGCCTTTCTG GCCATCGCCA AGGAACTGAA ATACCGGGCC GGGCATCAGG 661 CGGATGAGCC CAGCTTCCAG ATCCGAGACT ATGTAGAGTC CCAGAAGAAG CGCTCCAGCT 721 GCTGCTCCTT CATGTGCCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC 781 CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT 841 ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AACTCCGCCG CGTCAATCGC 901 GAACTACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt