Construct: ORF TRCN0000472102
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010772.1_s317c1
- Derived from:
- ccsbBroadEn_02753
- DNA Barcode:
- CAATCTACATGATTAGCGGGCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VPS39 (23339)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472102
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23339 | VPS39 | VPS39 subunit of HOPS complex | NM_015289.4 | 100% | 100% | |
| 2 | human | 23339 | VPS39 | VPS39 subunit of HOPS complex | NM_001301138.2 | 98.7% | 98.6% | 140_172del |
| 3 | human | 23339 | VPS39 | VPS39 subunit of HOPS complex | XM_017022037.1 | 97.7% | 96.2% | (many diffs) |
| 4 | human | 23339 | VPS39 | VPS39 subunit of HOPS complex | XM_011521404.2 | 97.1% | 94.8% | 2550_2619del;2688_2689insTGACACT |
| 5 | human | 23339 | VPS39 | VPS39 subunit of HOPS complex | XM_017022036.1 | 96.5% | 94.9% | (many diffs) |
| 6 | human | 23339 | VPS39 | VPS39 subunit of HOPS complex | XM_011521403.2 | 95.9% | 93.6% | 140_172del;2583_2652del;2721_2722insTGACACT |
| 7 | human | 23339 | VPS39 | VPS39 subunit of HOPS complex | XM_017022038.1 | 85.8% | 85.8% | 0_1ins372 |
| 8 | human | 23339 | VPS39 | VPS39 subunit of HOPS complex | XM_017022039.1 | 58.9% | 58.6% | (many diffs) |
| 9 | human | 23339 | VPS39 | VPS39 subunit of HOPS complex | XR_001751181.1 | 52.2% | 1_168del;2063_2237del;2969_5024del | |
| 10 | human | 23339 | VPS39 | VPS39 subunit of HOPS complex | XR_001751180.1 | 52.1% | (many diffs) | |
| 11 | mouse | 269338 | Vps39 | VPS39 HOPS complex subunit | NM_178851.3 | 91.1% | 97.3% | (many diffs) |
| 12 | mouse | 269338 | Vps39 | VPS39 HOPS complex subunit | NM_147153.3 | 89.9% | 96% | (many diffs) |
| 13 | mouse | 269338 | Vps39 | VPS39 HOPS complex subunit | XM_006499598.3 | 88.7% | 89.7% | (many diffs) |
| 14 | mouse | 269338 | Vps39 | VPS39 HOPS complex subunit | XR_001782888.1 | 55.7% | (many diffs) | |
| 15 | mouse | 269338 | Vps39 | VPS39 HOPS complex subunit | NR_027618.1 | 53.9% | (many diffs) | |
| 16 | mouse | 269338 | Vps39 | VPS39 HOPS complex subunit | XM_006499599.1 | 52.3% | 55.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2691
- ORF length:
- 2625
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca cgacgctttc gagccagtgc cgatcctaga aaagctgcct ctgcaaatcg 121 actgtctggc tgcctgggag gaatggcttc ttgtgggaac caaacaagga catcttcttc 181 tctataggat tcggaaggac gttggttgca acagatttga agtgacacta gagaaatcca 241 ataagaactt ctccaaaaag attcagcaga tccatgtggt ttcccagttt aagattctgg 301 tcagcttgtt agaaaataac atttatgtcc atgacctatt gacatttcaa caaatcacta 361 cggtttcaaa ggcaaaggga gcatcactgt ttacttgtga cctccagcac acagagaccg 421 gtgaggaggt gttacggatg tgtgtggcag taaaaaagaa gctgcagctc tatttctgga 481 aggacaggga atttcatgaa ttgcaggggg actttagtgt gccagatgtg cccaagtcca 541 tggcgtggtg tgaaaattct atctgtgtgg gtttcaagag agactactac ctaataaggg 601 tggatggaaa ggggtccatc aaagagctct ttccaacagg aaaacagctg gagcccttag 661 ttgcacctct ggcagatgga aaagtggctg tgggccagga tgatctcacc gtggtactca 721 atgaggaagg gatctgcaca cagaaatgtg ccctgaactg gacggacata ccagtggcca 781 tggagcacca gcctccctac atcattgcag tgttgcctcg atatgttgag atccgaacat 841 ttgaaccgag gcttctggtc caaagcattg aattgcaaag gccccgtttc attacctcag 901 gaggatcaaa cattatctat gtggccagca atcattttgt ttggagactc atccctgtcc 961 ccatggcaac ccaaatccaa caacttctcc aggacaagca gtttgaattg gctctgcagc 1021 tcgcagaaat gaaagatgat tctgacagtg aaaagcagca acaaattcat cacatcaaga 1081 acttgtatgc cttcaacctc ttctgccaga agcgttttga tgagtccatg caggtctttg 1141 ctaaacttgg cacagatccc acccatgtga tgggcctgta ccctgacctg ctgcccacag 1201 actacagaaa gcagttgcag tatcccaacc cattgcctgt gctctccggg gctgaattgg 1261 agaaggctca cttagctctg attgactacc tgacacagaa acgaagtcaa ttggtaaaga 1321 agctgaatga ctctgatcac cagtcaagca cctcaccgct catggaaggc actcccacca 1381 tcaaatccaa gaagaagctg ctacaaatca tcgacaccac cctgctcaag tgctatctcc 1441 atacaaatgt ggccctggtg gcccccttgc tacgcctgga gaacaatcac tgccacatcg 1501 aggagagcga gcacgtgcta aagaaggctc acaagtacag tgagcttatc atcctgtatg 1561 agaagaaggg gctccacgag aaagctctgc aggtgctcgt ggaccagtcc aagaaagcca 1621 actcccctct gaaaggccac gagaggacag tgcagtatct gcagcatctg ggcacagaaa 1681 acctgcattt gattttctcc tactcagtgt gggtgctgag agacttccca gaagatggcc 1741 tgaagatatt tactgaagat ctcccggaag tggagtctct gccacgtgat cgagtcctcg 1801 gcttcttaat agagaatttt aagggtctgg ctattcctta tctggaacac atcatccatg 1861 tttgggagga gacaggctct cggttccaca actgcctgat ccagctatac tgtgagaagg 1921 tgcaaggtct gatgaaggag tatctcctgt ccttccctgc aggcaaaacc ccagtcccag 1981 ctggagagga agagggtgag ctgggagaat accggcaaaa gctcctcatg ttcttggaga 2041 tttccagcta ctatgatcca ggccggctca tctgtgattt tccctttgat ggcctcttag 2101 aagaacgagc tctcctgttg gggcgcatgg ggaaacatga acaagctctt ttcatttatg 2161 tccacatctt gaaggataca aggatgGCTG AGGAGTACTG CCACAAACAC TATGACCGAA 2221 ACAAAGATGG CAACAAAGAT GTGTATCTGT CCCTGCTTCG GATGTACCTG TCGCCCCCCA 2281 GCATTCACTG CCTGGGGCCA ATCAAGCTGG AACTACTGGA GCCAAAAGCC AACCTCCAGG 2341 CCGCTCTGCA GGTCCTCGAG CTACACCACA GCAAACTGGA CACCACCAAG GCCCTCAACC 2401 TTCTGCCAGC AAACACTCAG ATCAATGACA TACGCATCTT CCTGGAAAAG GTCTTGGAAG 2461 AAAATGCACA AAAGAAACGG TTCAATCAAG TGCTCAAGAA CCTTCTCCAT GCAGAATTCC 2521 TGAGGGTCCA GGAAGAGCGG ATTTTACACC AGCAGGTGAA GTGCATCATC ACAGAGGAGA 2581 AGGTGTGCAT GGTGTGTAAG AAGAAGATTG GGAACAGTGC ATTTGCAAGA TACCCCAATG 2641 GAGTGGTCGT CCATTACTTC TGTTCCAAAG AGGTAAACCC AGCTGACACT TGCCCAACTT 2701 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2761 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2821 CTTGTGGAAA GGACGACAAT CTACATGATT AGCGGGCTCA ACGCGTTAAG TCgacaatca 2881 acctctggat tacaaaattt gtgaaagatt