Construct: ORF TRCN0000472102

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010772.1_s317c1
Derived from:
ccsbBroadEn_02753
DNA Barcode:
CAATCTACATGATTAGCGGGCTCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VPS39 (23339)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472102
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23339 VPS39 VPS39 subunit of HOPS complex NM_015289.4 100% 100%
2 human 23339 VPS39 VPS39 subunit of HOPS complex NM_001301138.2 98.7% 98.6% 140_172del
3 human 23339 VPS39 VPS39 subunit of HOPS complex XM_017022037.1 97.7% 96.2% (many diffs)
4 human 23339 VPS39 VPS39 subunit of HOPS complex XM_011521404.2 97.1% 94.8% 2550_2619del;2688_2689insTGACACT
5 human 23339 VPS39 VPS39 subunit of HOPS complex XM_017022036.1 96.5% 94.9% (many diffs)
6 human 23339 VPS39 VPS39 subunit of HOPS complex XM_011521403.2 95.9% 93.6% 140_172del;2583_2652del;2721_2722insTGACACT
7 human 23339 VPS39 VPS39 subunit of HOPS complex XM_017022038.1 85.8% 85.8% 0_1ins372
8 human 23339 VPS39 VPS39 subunit of HOPS complex XM_017022039.1 58.9% 58.6% (many diffs)
9 human 23339 VPS39 VPS39 subunit of HOPS complex XR_001751181.1 52.2% 1_168del;2063_2237del;2969_5024del
10 human 23339 VPS39 VPS39 subunit of HOPS complex XR_001751180.1 52.1% (many diffs)
11 mouse 269338 Vps39 VPS39 HOPS complex subunit NM_178851.3 91.1% 97.3% (many diffs)
12 mouse 269338 Vps39 VPS39 HOPS complex subunit NM_147153.3 89.9% 96% (many diffs)
13 mouse 269338 Vps39 VPS39 HOPS complex subunit XM_006499598.3 88.7% 89.7% (many diffs)
14 mouse 269338 Vps39 VPS39 HOPS complex subunit XR_001782888.1 55.7% (many diffs)
15 mouse 269338 Vps39 VPS39 HOPS complex subunit NR_027618.1 53.9% (many diffs)
16 mouse 269338 Vps39 VPS39 HOPS complex subunit XM_006499599.1 52.3% 55.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2691
ORF length:
2625
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca cgacgctttc gagccagtgc cgatcctaga aaagctgcct ctgcaaatcg
121actgtctggc tgcctgggag gaatggcttc ttgtgggaac caaacaagga catcttcttc
181tctataggat tcggaaggac gttggttgca acagatttga agtgacacta gagaaatcca
241ataagaactt ctccaaaaag attcagcaga tccatgtggt ttcccagttt aagattctgg
301tcagcttgtt agaaaataac atttatgtcc atgacctatt gacatttcaa caaatcacta
361cggtttcaaa ggcaaaggga gcatcactgt ttacttgtga cctccagcac acagagaccg
421gtgaggaggt gttacggatg tgtgtggcag taaaaaagaa gctgcagctc tatttctgga
481aggacaggga atttcatgaa ttgcaggggg actttagtgt gccagatgtg cccaagtcca
541tggcgtggtg tgaaaattct atctgtgtgg gtttcaagag agactactac ctaataaggg
601tggatggaaa ggggtccatc aaagagctct ttccaacagg aaaacagctg gagcccttag
661ttgcacctct ggcagatgga aaagtggctg tgggccagga tgatctcacc gtggtactca
721atgaggaagg gatctgcaca cagaaatgtg ccctgaactg gacggacata ccagtggcca
781tggagcacca gcctccctac atcattgcag tgttgcctcg atatgttgag atccgaacat
841ttgaaccgag gcttctggtc caaagcattg aattgcaaag gccccgtttc attacctcag
901gaggatcaaa cattatctat gtggccagca atcattttgt ttggagactc atccctgtcc
961ccatggcaac ccaaatccaa caacttctcc aggacaagca gtttgaattg gctctgcagc
1021tcgcagaaat gaaagatgat tctgacagtg aaaagcagca acaaattcat cacatcaaga
1081acttgtatgc cttcaacctc ttctgccaga agcgttttga tgagtccatg caggtctttg
1141ctaaacttgg cacagatccc acccatgtga tgggcctgta ccctgacctg ctgcccacag
1201actacagaaa gcagttgcag tatcccaacc cattgcctgt gctctccggg gctgaattgg
1261agaaggctca cttagctctg attgactacc tgacacagaa acgaagtcaa ttggtaaaga
1321agctgaatga ctctgatcac cagtcaagca cctcaccgct catggaaggc actcccacca
1381tcaaatccaa gaagaagctg ctacaaatca tcgacaccac cctgctcaag tgctatctcc
1441atacaaatgt ggccctggtg gcccccttgc tacgcctgga gaacaatcac tgccacatcg
1501aggagagcga gcacgtgcta aagaaggctc acaagtacag tgagcttatc atcctgtatg
1561agaagaaggg gctccacgag aaagctctgc aggtgctcgt ggaccagtcc aagaaagcca
1621actcccctct gaaaggccac gagaggacag tgcagtatct gcagcatctg ggcacagaaa
1681acctgcattt gattttctcc tactcagtgt gggtgctgag agacttccca gaagatggcc
1741tgaagatatt tactgaagat ctcccggaag tggagtctct gccacgtgat cgagtcctcg
1801gcttcttaat agagaatttt aagggtctgg ctattcctta tctggaacac atcatccatg
1861tttgggagga gacaggctct cggttccaca actgcctgat ccagctatac tgtgagaagg
1921tgcaaggtct gatgaaggag tatctcctgt ccttccctgc aggcaaaacc ccagtcccag
1981ctggagagga agagggtgag ctgggagaat accggcaaaa gctcctcatg ttcttggaga
2041tttccagcta ctatgatcca ggccggctca tctgtgattt tccctttgat ggcctcttag
2101aagaacgagc tctcctgttg gggcgcatgg ggaaacatga acaagctctt ttcatttatg
2161tccacatctt gaaggataca aggatgGCTG AGGAGTACTG CCACAAACAC TATGACCGAA
2221ACAAAGATGG CAACAAAGAT GTGTATCTGT CCCTGCTTCG GATGTACCTG TCGCCCCCCA
2281GCATTCACTG CCTGGGGCCA ATCAAGCTGG AACTACTGGA GCCAAAAGCC AACCTCCAGG
2341CCGCTCTGCA GGTCCTCGAG CTACACCACA GCAAACTGGA CACCACCAAG GCCCTCAACC
2401TTCTGCCAGC AAACACTCAG ATCAATGACA TACGCATCTT CCTGGAAAAG GTCTTGGAAG
2461AAAATGCACA AAAGAAACGG TTCAATCAAG TGCTCAAGAA CCTTCTCCAT GCAGAATTCC
2521TGAGGGTCCA GGAAGAGCGG ATTTTACACC AGCAGGTGAA GTGCATCATC ACAGAGGAGA
2581AGGTGTGCAT GGTGTGTAAG AAGAAGATTG GGAACAGTGC ATTTGCAAGA TACCCCAATG
2641GAGTGGTCGT CCATTACTTC TGTTCCAAAG AGGTAAACCC AGCTGACACT TGCCCAACTT
2701TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
2761CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
2821CTTGTGGAAA GGACGACAAT CTACATGATT AGCGGGCTCA ACGCGTTAAG TCgacaatca
2881acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)