Construct: ORF TRCN0000472152
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006852.1_s317c1
- Derived from:
- ccsbBroadEn_01045
- DNA Barcode:
- TGCCGATATAGCTCTCTATACTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MT2A (4502)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472152
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4502 | MT2A | metallothionein 2A | NM_005953.5 | 100% | 100% | |
| 2 | human | 4495 | MT1G | metallothionein 1G | NM_005950.3 | 95% | 95% | (many diffs) |
| 3 | human | 4495 | MT1G | metallothionein 1G | NM_001301267.2 | 93.5% | 93.5% | (many diffs) |
| 4 | human | 4501 | MT1X | metallothionein 1X | NM_005952.4 | 92.9% | 90.1% | (many diffs) |
| 5 | human | 4493 | MT1E | metallothionein 1E | NM_175617.4 | 92.3% | 93.4% | (many diffs) |
| 6 | human | 4489 | MT1A | metallothionein 1A | NM_005946.3 | 92.3% | 90.1% | (many diffs) |
| 7 | human | 4496 | MT1H | metallothionein 1H | NM_005951.2 | 91.8% | 90.1% | (many diffs) |
| 8 | human | 4494 | MT1F | metallothionein 1F | NM_005949.4 | 91.2% | 91.8% | (many diffs) |
| 9 | human | 645745 | MT1HL1 | metallothionein 1H like 1 | NM_001276687.2 | 91.2% | 88.5% | (many diffs) |
| 10 | human | 4499 | MT1M | metallothionein 1M | NM_176870.3 | 89.6% | 80.3% | (many diffs) |
| 11 | human | 4490 | MT1B | metallothionein 1B | NM_005947.3 | 88.5% | 85.2% | (many diffs) |
| 12 | human | 4494 | MT1F | metallothionein 1F | NM_001301272.1 | 56.7% | 53.9% | (many diffs) |
| 13 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_027781.1 | 43% | (many diffs) | |
| 14 | human | 4498 | MT1JP | metallothionein 1J, pseudogene | NR_036677.1 | 40.6% | (many diffs) | |
| 15 | human | 4500 | MT1L | metallothionein 1L, pseudogene | NR_001447.2 | 37.6% | (many diffs) | |
| 16 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_003669.2 | 36.4% | (many diffs) | |
| 17 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_003658.2 | 30.9% | (many diffs) | |
| 18 | mouse | 17750 | Mt2 | metallothionein 2 | NM_008630.2 | 88.5% | 86.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 272
- ORF end:
- 455
- ORF length:
- 183
- Sequence:
-
1 CACCTCGATT AGTTCTCGAG CTTTTGGAGT ACGTCGTCTT TAGGTTGGGG GGAGGGGTTT 61 TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC 121 TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG 181 CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA 241 TTGATCAACA AGTTTGTACA AAAAAGTTGG CATGGATCCC AACTGCTCCT GCGCCGCCGG 301 TGACTCCTGC ACCTGCGCCG GCTCCTGCAA ATGCAAAGAG TGCAAATGCA CCTCCTGCAA 361 GAAAAGCTGC TGCTCCTGCT GCCCTGTGGG CTGTGCCAAG TGTGCCCAGG GCTGCATCTG 421 CAAAGGGGCG TCGGACAAGT GCAGCTGCTG CGCCTGCCCA ACTTTCTTGT ACAAAGTGGT 481 TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC 541 TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 601 TGCCGATATA GCTCTCTATA CTCAACGCGT TAAGTCgaca atcaacctct ggattacaaa 661 atttgtgaaa gatt