Transcript: Mouse XM_006502056.2

PREDICTED: Mus musculus DnaJ heat shock protein family (Hsp40) member B14 (Dnajb14), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Dnajb14 (70604)
Length:
9033
CDS:
1..939

Additional Resources:

NCBI RefSeq record:
XM_006502056.2
NBCI Gene record:
Dnajb14 (70604)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006502056.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000341981 CGTGACTAATATTCGAAATAA pLKO_005 771 CDS 100% 15.000 21.000 N Dnajb14 n/a
2 TRCN0000335864 GTGACTAATATTCGAAATAAC pLKO_005 772 CDS 100% 13.200 18.480 N DNAJB14 n/a
3 TRCN0000341983 TCCCGAAGACCTGTTCAATAT pLKO_005 390 CDS 100% 13.200 18.480 N Dnajb14 n/a
4 TRCN0000342056 CTACGAAGTCCTCGGAGTTAC pLKO_005 126 CDS 100% 10.800 15.120 N Dnajb14 n/a
5 TRCN0000341984 TACGTCAGTAAGGACTTTAAA pLKO_005 694 CDS 100% 15.000 10.500 N Dnajb14 n/a
6 TRCN0000341982 CAAAGTCTGGAGAGGATTATC pLKO_005 1164 3UTR 100% 13.200 9.240 N Dnajb14 n/a
7 TRCN0000152147 CGAAATAACTGCTGGAAAGAA pLKO.1 784 CDS 100% 5.625 3.938 N DNAJB14 n/a
8 TRCN0000106535 GCCTGGTCTACAAAGTGAGTT pLKO.1 1762 3UTR 100% 4.950 2.475 Y Gad2 n/a
9 TRCN0000182745 CCAGAGTTCAAATCCCAGCAA pLKO.1 6581 3UTR 100% 2.640 1.320 Y P3h3 n/a
10 TRCN0000320070 CCAGAGTTCAAATCCCAGCAA pLKO_005 6581 3UTR 100% 2.640 1.320 Y P3h3 n/a
11 TRCN0000150360 CCTGAAATCTTGGACTGTTTA pLKO.1 1046 3UTR 100% 13.200 9.240 N DNAJB14 n/a
12 TRCN0000335860 CCTGAAATCTTGGACTGTTTA pLKO_005 1046 3UTR 100% 13.200 9.240 N DNAJB14 n/a
13 TRCN0000054408 ACGCCTTTAATCCCAGCACTT pLKO.1 1703 3UTR 100% 4.050 2.025 Y Mtif2 n/a
14 TRCN0000088533 CGGGAGGCAGAGGCAGGCAAT pLKO.1 1723 3UTR 100% 0.000 0.000 Y Trrap n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006502056.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04159 pDONR223 100% 71.7% 71.5% None (many diffs) n/a
2 ccsbBroad304_04159 pLX_304 0% 71.7% 71.5% V5 (many diffs) n/a
3 TRCN0000472243 TCTCTCTGACATTTTGCCTTCCTT pLX_317 39.2% 71.7% 71.5% V5 (many diffs) n/a
4 ccsbBroadEn_13727 pDONR223 100% 34.6% 35.8% None (many diffs) n/a
5 ccsbBroad304_13727 pLX_304 0% 34.6% 35.8% V5 (many diffs) n/a
6 TRCN0000479965 TGGTTTCTCTAACACGCTCCACAC pLX_317 40.6% 34.6% 35.8% V5 (many diffs) n/a
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