Construct: ORF TRCN0000472534
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005842.1_s317c1
- Derived from:
- ccsbBroadEn_15634
- DNA Barcode:
- GTTAGCTAACGAGCGGGCCAGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HAT1 (8520)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472534
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8520 | HAT1 | histone acetyltransferase 1 | XM_006712808.3 | 43.2% | 43% | 1_702del;923T>C |
| 2 | human | 8520 | HAT1 | histone acetyltransferase 1 | XM_017005136.2 | 43.2% | 43% | 1_702del;923T>C |
| 3 | human | 8520 | HAT1 | histone acetyltransferase 1 | XM_017005137.2 | 43.2% | 43% | 1_702del;923T>C |
| 4 | human | 8520 | HAT1 | histone acetyltransferase 1 | XM_024453186.1 | 43.2% | 43% | 1_702del;923T>C |
| 5 | human | 8520 | HAT1 | histone acetyltransferase 1 | XM_024453187.1 | 43.2% | 43% | 1_702del;923T>C |
| 6 | human | 8520 | HAT1 | histone acetyltransferase 1 | NM_003642.4 | 42.6% | 42.4% | 1_720del;941T>C |
| 7 | human | 8520 | HAT1 | histone acetyltransferase 1 | NR_027862.1 | 33.3% | 1_720del;1258_1609del | |
| 8 | mouse | 107435 | Hat1 | histone aminotransferase 1 | NM_026115.4 | 38.7% | 39.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 603
- ORF length:
- 537
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gattttgact ccatttcaag gtcaaggcca tggtgctcaa cttcttgaaa 121 cagttcatag atactacact gaatttccTA CAGTTCTTGA TATTACAGCG GAAGATCCAT 181 CCAAAAGCTA TGTGAAATTA CGAGACTTTG TGCTTGTGAA GCTTTGTCAA GATTTGCCCT 241 GTTTTTCCCG GGAAAAATTA ATGCAAGGAT TCAATGAAGA TATGGCGATA GAGGCACAAC 301 AGAAGTTCAA AATAAATAAG CAACACGCTA GAAGGGTTTA TGAAATTCTT CGACTACTGG 361 TAACTGACAT GAGTGATGCC GAACAATACA GAAGCTACAG ACTGGATATT AAAAGAAGAC 421 TAATTAGCCC ATATAAGAAA AAGCAGAGAG ATCTTGCTAA GATGAGAAAA TGTCTCAGAC 481 CAGAAGAACT GACAAACCAG ATGAACCAAA TAGAAATAAG CATGCAACAT GAACAGCTGG 541 AAGAGAGTTT TCAGGAACTA GTGGAAGATT ACCGGCGTGT TATTGAACGA CTTGCTCAAG 601 AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 661 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 721 GGCTTTATAT ATCTTGTGGA AAGGACGAGT TAGCTAACGA GCGGGCCAGA CGACGCGTTA 781 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt