Construct: ORF TRCN0000472827

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014630.1_s317c1
Derived from:
ccsbBroadEn_08662
DNA Barcode:
CCGGAGTCCTGCCACCGGATAATG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MCCC1 (56922)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472827
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56922 MCCC1 methylcrotonoyl-CoA carboxy... NM_020166.5 99.9% 99.8% 396C>T;1391A>C
2 human 56922 MCCC1 methylcrotonoyl-CoA carboxy... XM_011512992.2 94.6% 94.6% 0_1ins114;282C>T;1277A>C
3 human 56922 MCCC1 methylcrotonoyl-CoA carboxy... XR_001740208.2 85% (many diffs)
4 human 56922 MCCC1 methylcrotonoyl-CoA carboxy... NM_001363880.1 84.8% 84.8% 0_1ins327;69C>T;1064A>C
5 human 56922 MCCC1 methylcrotonoyl-CoA carboxy... XR_001740207.2 84.7% (many diffs)
6 human 56922 MCCC1 methylcrotonoyl-CoA carboxy... NM_001293273.1 83.8% 77.5% (many diffs)
7 human 56922 MCCC1 methylcrotonoyl-CoA carboxy... XR_001740210.1 81.2% (many diffs)
8 human 56922 MCCC1 methylcrotonoyl-CoA carboxy... XR_241502.3 78.7% (many diffs)
9 human 56922 MCCC1 methylcrotonoyl-CoA carboxy... XR_001740209.2 76.7% (many diffs)
10 human 56922 MCCC1 methylcrotonoyl-CoA carboxy... NR_120639.1 73.7% (many diffs)
11 human 56922 MCCC1 methylcrotonoyl-CoA carboxy... XR_002959554.1 63.1% (many diffs)
12 human 56922 MCCC1 methylcrotonoyl-CoA carboxy... NR_120640.1 59% (many diffs)
13 human 56922 MCCC1 methylcrotonoyl-CoA carboxy... XR_002959553.1 33.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2241
ORF length:
2175
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggcggcctct gcggtgtcgg tgctgctggt ggcggcggag aggaaccggt
121ggcatcgtct cccgagcctg ctcctgccgc cgaggacatg ggtgtggagg caaagaacca
181tgaagtacac aacagccaca ggaagaaaca ttaccaaggt cctcattgca aacagaggag
241aaattgcctg cagggtgatg cgcacagcca aaaaactggg tgtacagact gtggcggttt
301atagtgaggc tgacagaaat tccatgcatg tagatatggc agatgaagca tattccatcg
361gccccgctcc ctcccagcag agctacctat ctatggagaa aatcattcaa gtggccaaga
421cctctgctgc acaggctatc catccaggat gcggttttct ttcagaaaac atggaatttg
481ctgaactttg taagcaagaa ggaattattt ttataggccc tcctccatct gcaattagag
541acatgggtat aaagagcaca tccaaatcca taatggctgc tgctggagta cctgttgtgg
601agggttatca tggtgaggac caatcagacc agtgcctgaa ggaacacgcc aggagaattg
661gctatcctgt catgattaaa gccgtccggg gtggaggagg aaaaggaatg aggattgtta
721gatcagaaca agaatttcaa gaacagttag agtcagcacg gagagaagct aagaagtctt
781tcaatgatga tgctatgctg atcgagaagt ttgtagacac accgaggcat gtagaagtcc
841aggtgtttgg tgatcaccat ggcaatgctg tgtacttgtt tgaaagagac tgtagtgtgc
901agaggcgaca tcagaagatc attgaggagg ccccagcgcc tggtattaaa tctgaagtaa
961gaaaaaagct gggagaagct gcagtcagag ctgctaaagc tgtaaattat gttggagcag
1021ggactgtgga gtttattatg gactcaaaac ataatttctg tttcatggag atgaatacaa
1081ggctgcaagt ggaacatcct gttactgaga tgatcacagg aactgacttg gtggagtggc
1141agcttagaat tgcagcagga gagaagattc ctttgagcca ggaagaaata actctgcagg
1201gccatgcctt cgaagctaga atatatgcag aagatcctag caataacttc atgcctgtgg
1261caggcccatt agtgcacctc tctactcctc gagcagaccc ttccaccagg attgaaactg
1321gagtacggca aggagacgaa gtttccgtgc attatgaccc catgattgcg aagctggtcg
1381tgtgggcagc agatcgccag gcggcattga caaaactgag gtacagcctt cgtcagtaca
1441atattgttgg actgcccacc aacattgact tcttactcaa cctgtctggc cacccagagt
1501ttgaagctgg gaacgtgcac actgatttca tccctcaaca ccacaaacag ttgttgctca
1561gtcggaaggc tgcagccaaa gagtctttat gccaggcagc cctgggtctc atcctcaagg
1621agaaagccat gaccgacact ttcactcttc aggcacatga tcaattctct ccattttcgt
1681ctagcagtgg aagaagactg aatatctcgt ataccagaaa catgactctt aaagatggta
1741aaaacaatgt agccatagct gtaacgtata accatgatgg gtcttatagc atgcagattg
1801aagataaaac tttccaagtc cttggtaaTC TTTACAGCGA GGGAGACTGC ACTTACCTGA
1861AATGTTCTGT TAATGGAGTT GCTAGTAAAG CGAAGCTGAT TATCCTGGAA AACACTATTT
1921ACCTATTTTC CAAGGAAGGA AGTATTGAGA TTGACATTCC AGTCCCCAAA TACTTATCTT
1981CTGTGAGCTC ACAAGAAACT CAGGGCGGCC CCTTAGCTCC TATGACTGGA ACCATTGAAA
2041AGGTGTTTGT CAAAGCTGGA GACAAAGTGA AAGCGGGAGA TTCCCTCATG GTTATGATCG
2101CCATGAAGAT GGAGCATACC ATAAAGTCTC CAAAGGATGG CACAGTAAAG AAAGTGTTCT
2161ACAGAGAAGG TGCTCAGGCC AACAGACACA CTCCTTTAGT CGAGTTTGAG GAGGAAGAAT
2221CAGACAAAAG GGAATCGGAA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
2281CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
2341AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCGG AGTCCTGCCA
2401CCGGATAATG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)