Construct: ORF TRCN0000472827
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014630.1_s317c1
- Derived from:
- ccsbBroadEn_08662
- DNA Barcode:
- CCGGAGTCCTGCCACCGGATAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCCC1 (56922)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472827
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56922 | MCCC1 | methylcrotonoyl-CoA carboxy... | NM_020166.5 | 99.9% | 99.8% | 396C>T;1391A>C |
| 2 | human | 56922 | MCCC1 | methylcrotonoyl-CoA carboxy... | XM_011512992.2 | 94.6% | 94.6% | 0_1ins114;282C>T;1277A>C |
| 3 | human | 56922 | MCCC1 | methylcrotonoyl-CoA carboxy... | XR_001740208.2 | 85% | (many diffs) | |
| 4 | human | 56922 | MCCC1 | methylcrotonoyl-CoA carboxy... | NM_001363880.1 | 84.8% | 84.8% | 0_1ins327;69C>T;1064A>C |
| 5 | human | 56922 | MCCC1 | methylcrotonoyl-CoA carboxy... | XR_001740207.2 | 84.7% | (many diffs) | |
| 6 | human | 56922 | MCCC1 | methylcrotonoyl-CoA carboxy... | NM_001293273.1 | 83.8% | 77.5% | (many diffs) |
| 7 | human | 56922 | MCCC1 | methylcrotonoyl-CoA carboxy... | XR_001740210.1 | 81.2% | (many diffs) | |
| 8 | human | 56922 | MCCC1 | methylcrotonoyl-CoA carboxy... | XR_241502.3 | 78.7% | (many diffs) | |
| 9 | human | 56922 | MCCC1 | methylcrotonoyl-CoA carboxy... | XR_001740209.2 | 76.7% | (many diffs) | |
| 10 | human | 56922 | MCCC1 | methylcrotonoyl-CoA carboxy... | NR_120639.1 | 73.7% | (many diffs) | |
| 11 | human | 56922 | MCCC1 | methylcrotonoyl-CoA carboxy... | XR_002959554.1 | 63.1% | (many diffs) | |
| 12 | human | 56922 | MCCC1 | methylcrotonoyl-CoA carboxy... | NR_120640.1 | 59% | (many diffs) | |
| 13 | human | 56922 | MCCC1 | methylcrotonoyl-CoA carboxy... | XR_002959553.1 | 33.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2241
- ORF length:
- 2175
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggcctct gcggtgtcgg tgctgctggt ggcggcggag aggaaccggt 121 ggcatcgtct cccgagcctg ctcctgccgc cgaggacatg ggtgtggagg caaagaacca 181 tgaagtacac aacagccaca ggaagaaaca ttaccaaggt cctcattgca aacagaggag 241 aaattgcctg cagggtgatg cgcacagcca aaaaactggg tgtacagact gtggcggttt 301 atagtgaggc tgacagaaat tccatgcatg tagatatggc agatgaagca tattccatcg 361 gccccgctcc ctcccagcag agctacctat ctatggagaa aatcattcaa gtggccaaga 421 cctctgctgc acaggctatc catccaggat gcggttttct ttcagaaaac atggaatttg 481 ctgaactttg taagcaagaa ggaattattt ttataggccc tcctccatct gcaattagag 541 acatgggtat aaagagcaca tccaaatcca taatggctgc tgctggagta cctgttgtgg 601 agggttatca tggtgaggac caatcagacc agtgcctgaa ggaacacgcc aggagaattg 661 gctatcctgt catgattaaa gccgtccggg gtggaggagg aaaaggaatg aggattgtta 721 gatcagaaca agaatttcaa gaacagttag agtcagcacg gagagaagct aagaagtctt 781 tcaatgatga tgctatgctg atcgagaagt ttgtagacac accgaggcat gtagaagtcc 841 aggtgtttgg tgatcaccat ggcaatgctg tgtacttgtt tgaaagagac tgtagtgtgc 901 agaggcgaca tcagaagatc attgaggagg ccccagcgcc tggtattaaa tctgaagtaa 961 gaaaaaagct gggagaagct gcagtcagag ctgctaaagc tgtaaattat gttggagcag 1021 ggactgtgga gtttattatg gactcaaaac ataatttctg tttcatggag atgaatacaa 1081 ggctgcaagt ggaacatcct gttactgaga tgatcacagg aactgacttg gtggagtggc 1141 agcttagaat tgcagcagga gagaagattc ctttgagcca ggaagaaata actctgcagg 1201 gccatgcctt cgaagctaga atatatgcag aagatcctag caataacttc atgcctgtgg 1261 caggcccatt agtgcacctc tctactcctc gagcagaccc ttccaccagg attgaaactg 1321 gagtacggca aggagacgaa gtttccgtgc attatgaccc catgattgcg aagctggtcg 1381 tgtgggcagc agatcgccag gcggcattga caaaactgag gtacagcctt cgtcagtaca 1441 atattgttgg actgcccacc aacattgact tcttactcaa cctgtctggc cacccagagt 1501 ttgaagctgg gaacgtgcac actgatttca tccctcaaca ccacaaacag ttgttgctca 1561 gtcggaaggc tgcagccaaa gagtctttat gccaggcagc cctgggtctc atcctcaagg 1621 agaaagccat gaccgacact ttcactcttc aggcacatga tcaattctct ccattttcgt 1681 ctagcagtgg aagaagactg aatatctcgt ataccagaaa catgactctt aaagatggta 1741 aaaacaatgt agccatagct gtaacgtata accatgatgg gtcttatagc atgcagattg 1801 aagataaaac tttccaagtc cttggtaaTC TTTACAGCGA GGGAGACTGC ACTTACCTGA 1861 AATGTTCTGT TAATGGAGTT GCTAGTAAAG CGAAGCTGAT TATCCTGGAA AACACTATTT 1921 ACCTATTTTC CAAGGAAGGA AGTATTGAGA TTGACATTCC AGTCCCCAAA TACTTATCTT 1981 CTGTGAGCTC ACAAGAAACT CAGGGCGGCC CCTTAGCTCC TATGACTGGA ACCATTGAAA 2041 AGGTGTTTGT CAAAGCTGGA GACAAAGTGA AAGCGGGAGA TTCCCTCATG GTTATGATCG 2101 CCATGAAGAT GGAGCATACC ATAAAGTCTC CAAAGGATGG CACAGTAAAG AAAGTGTTCT 2161 ACAGAGAAGG TGCTCAGGCC AACAGACACA CTCCTTTAGT CGAGTTTGAG GAGGAAGAAT 2221 CAGACAAAAG GGAATCGGAA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2281 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2341 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCGG AGTCCTGCCA 2401 CCGGATAATG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt