Construct: ORF TRCN0000473445
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012301.1_s317c1
- Derived from:
- ccsbBroadEn_11320
- DNA Barcode:
- ATCTCCGTTGTCTGGATAATCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FUBP3 (8939)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473445
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8939 | FUBP3 | far upstream element bindin... | XM_011519172.3 | 48.4% | 46.2% | (many diffs) |
| 2 | human | 8939 | FUBP3 | far upstream element bindin... | XM_011519173.2 | 46.6% | 44.3% | (many diffs) |
| 3 | human | 8939 | FUBP3 | far upstream element bindin... | XM_017015269.1 | 46.1% | 47.9% | (many diffs) |
| 4 | human | 8939 | FUBP3 | far upstream element bindin... | XM_005272232.2 | 45.5% | 46.8% | (many diffs) |
| 5 | human | 8939 | FUBP3 | far upstream element bindin... | XM_006717313.2 | 44% | 42% | (many diffs) |
| 6 | human | 8939 | FUBP3 | far upstream element bindin... | XM_011519174.2 | 44% | 42% | (many diffs) |
| 7 | human | 8939 | FUBP3 | far upstream element bindin... | NM_003934.2 | 43.6% | 41.6% | (many diffs) |
| 8 | human | 8939 | FUBP3 | far upstream element bindin... | XM_017015273.1 | 24.2% | 25.8% | (many diffs) |
| 9 | human | 8939 | FUBP3 | far upstream element bindin... | XR_001746412.2 | 23.8% | (many diffs) | |
| 10 | human | 8939 | FUBP3 | far upstream element bindin... | XR_001746411.2 | 23.3% | (many diffs) | |
| 11 | human | 8939 | FUBP3 | far upstream element bindin... | XM_017015270.1 | 23.1% | 21.1% | (many diffs) |
| 12 | human | 8939 | FUBP3 | far upstream element bindin... | XM_017015271.1 | 23.1% | 21.1% | (many diffs) |
| 13 | human | 8939 | FUBP3 | far upstream element bindin... | XM_017015272.1 | 23.1% | 21.1% | (many diffs) |
| 14 | human | 8939 | FUBP3 | far upstream element bindin... | XR_001746413.1 | 17.3% | (many diffs) | |
| 15 | mouse | 320267 | Fubp3 | far upstream element (FUSE)... | XM_017319016.1 | 41.4% | 45.1% | (many diffs) |
| 16 | mouse | 320267 | Fubp3 | far upstream element (FUSE)... | XM_006498129.1 | 40.9% | 41.1% | (many diffs) |
| 17 | mouse | 320267 | Fubp3 | far upstream element (FUSE)... | XM_017319015.1 | 39.7% | 39.9% | (many diffs) |
| 18 | mouse | 320267 | Fubp3 | far upstream element (FUSE)... | NM_001290548.1 | 39.6% | 39.8% | (many diffs) |
| 19 | mouse | 320267 | Fubp3 | far upstream element (FUSE)... | XM_006498127.1 | 39.3% | 39.5% | (many diffs) |
| 20 | mouse | 320267 | Fubp3 | far upstream element (FUSE)... | NM_001033389.4 | 39.2% | 39.4% | (many diffs) |
| 21 | mouse | 320267 | Fubp3 | far upstream element (FUSE)... | XM_006498126.1 | 39.2% | 39.4% | (many diffs) |
| 22 | mouse | 320267 | Fubp3 | far upstream element (FUSE)... | XR_001783041.1 | 20% | (many diffs) | |
| 23 | mouse | 320267 | Fubp3 | far upstream element (FUSE)... | XR_001783039.1 | 18.1% | (many diffs) | |
| 24 | mouse | 320267 | Fubp3 | far upstream element (FUSE)... | XR_001783038.1 | 17.8% | (many diffs) | |
| 25 | mouse | 320267 | Fubp3 | far upstream element (FUSE)... | XR_001783040.1 | 17.2% | (many diffs) | |
| 26 | mouse | 320267 | Fubp3 | far upstream element (FUSE)... | XR_001783042.1 | 16.6% | (many diffs) | |
| 27 | mouse | 320267 | Fubp3 | far upstream element (FUSE)... | XR_001783037.1 | 15.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 849
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tcctataggt aaccagttag gggccttggt acatcaaagg acggtaataa 121 cggaagaatt caaagtgcct gacaaaatgg ttggatttat tatcggcagg ggaggtgagc 181 agatttcacg gattcaagca gaatctggtt gcaaaattca gattgcttca gagagttctg 241 ggattccaga gaggccctgt gtacttaccg gaaccccaga aagtattgaa caagccaaac 301 ggctcctggg acagattgtg gaccgctgtc gaaatggacc tggctttcat aatgacatag 361 acagcaacag cacaatccag gagattctca ttcccgcatc taaagtgggt ctggtcatcg 421 gcagaggagg ggaaacaatc aagcagttgc aggagcgGAC AGGGGTGAAG ATGGTCATGA 481 TCCAGGATGG CCCATTGCCC ACGGGAGCAG ACAAGCCTCT TCGTATCACT GGAGATGCAT 541 TTAAAGTACA GCAAGCAAGA GAAATGGTAC TAGAGATTAT CCGAGAAAAA GACCAAGCTG 601 ACTTTCGGGG TGTACGCGGC GATTTCAACT CTCGAATGGG AGGAGGCAGT ATAGAGGTAT 661 CTGTGCCTAG GTTTGCTGTG GGGATTGTAA TAGGAAGAAA CGGGGAAATG ATCAAAAAGA 721 TCCAGAATGA TGCTGGTGTG AGGATTCAGT TTAAACCAGA TGATGGGATT AGTCCAGAAT 781 ATTACAGACA GCAGGTCGCT TTCTACGGAC AGACGTTAGG GCAGGCGCAG GCCCACAGCC 841 AGGAGCAGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 901 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 961 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATCTCC GTTGTCTGGA TAATCCTTAC 1021 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt