Construct: ORF TRCN0000473910
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013508.1_s317c1
- Derived from:
- ccsbBroadEn_08607
- DNA Barcode:
- CTTTCGTGTGTTCAGTATAAGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYNN (55892)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473910
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55892 | MYNN | myoneurin | NM_001185118.2 | 99.9% | 99.8% | 5A>G |
| 2 | human | 55892 | MYNN | myoneurin | NM_018657.5 | 99.9% | 99.8% | 5A>G |
| 3 | human | 55892 | MYNN | myoneurin | NM_001185119.1 | 95.1% | 95% | 5A>G;1482_1483ins87 |
| 4 | human | 55892 | MYNN | myoneurin | NR_033703.2 | 34.4% | (many diffs) | |
| 5 | human | 55892 | MYNN | myoneurin | NR_033702.2 | 34.1% | (many diffs) | |
| 6 | mouse | 80732 | Mynn | myoneurin | NM_030557.3 | 87.8% | 91.8% | (many diffs) |
| 7 | mouse | 80732 | Mynn | myoneurin | NM_001289621.1 | 83.2% | 87.2% | (many diffs) |
| 8 | mouse | 80732 | Mynn | myoneurin | NM_001289622.1 | 70.4% | 73.4% | (many diffs) |
| 9 | mouse | 80732 | Mynn | myoneurin | NM_001289623.1 | 65.9% | 68.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1896
- ORF length:
- 1830
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gtattcgcac cactgtgagc accttttaga gagactgaac aaacagcggg 121 aagcaggttt tctctgtgac tgtaccatag tgattgggga attccagttt aaagctcata 181 ggaatgtgct ggcctccttt agtgagtatt ttggtgcgat ctacagaagc acttctgaga 241 acaatgtctt tcttgatcag agtcaggtga aggctgatgg atttcagaaa ctgttggagt 301 ttatatacac aggaacttta aatcttgaca gttggaatgt taaagaaatt catcaggctg 361 ctgactatct caaagtggaa gaggtggtca ctaaatgcaa aataaagatg gaagattttg 421 cttttattgc taatccttct tctacagaga tatctagtat tactggaaac attgaattga 481 atcaacagac ttgtcttctt actctgcgag attataataa tcgagagaaa tcagaagtat 541 ctacagattt gattcaggca aatcctaaac aaggcgcgtt agcgaaaaag tcatctcaaa 601 cgaaaaagaa gaagaaggct ttcaactccc cgaaaacagg gcagaataaa acagtgcaat 661 atcccagtga catcttagag aatgcatctg ttgaattatt cctagatgca aataaactgc 721 ccacacctgt agtagaacaa gttgcacaaa taaatgataa ttcagaactc gagttgacat 781 cagttgtgga aaatactttt ccagcacaag atattgtgca cactgttaca gtgaaacgga 841 aacgtggaaa atcacagcca aactgtgctc tgaaagaaca ctctatgtct aatatagcca 901 gcgtcaagag tccttatgag gcggagaact ccggggaaga gctggatcag aggtattcca 961 aggccaagcc aatgtgtaac acatgtggga aagtgttttc agaagccagc agtttgagaa 1021 ggcacatgag aatacataaa ggagtcaaac cttacgtctg ccacttatgt ggaaaggcat 1081 ttacccaatg taaccagctg aaaacgcatg taagaactca tacaggtgag aagccataca 1141 aatgtgaatt gtgtgataaa ggatttgctc agaaatgtca gctagtcttc catagtcgca 1201 tgcatcatgg tgaagaaaaa ccctataaat gtgatgtatg caacttacag tttgcaactt 1261 ctagcaatct caagattcat gcaaggaagc atagtggaga gaagccatat gtctgtgata 1321 ggtgtggaca gagatttgct caagccagca cactgaccta tcatgtccgt aggcatactg 1381 gagaaaagcc ttatgtatgt gatacctgtg ggaaggcatt tgctgtctct agttctctta 1441 tcactcattc tcgaaaacat acaggtgaaa aaccatacat atgtggtatt tgtgggaaaa 1501 gttttatttc cTCAGGAGAG CTCAACAAAC ACTTTCGGTC CCATACAGGA GAAAGACCAT 1561 TTATCTGCGA ATTATGTGGA AATTCTTACA CAGATATTAA AAATTTAAAG AAGCACAAAA 1621 CAAAAGTCCA TTCTGGTGCA GATAAAACTC TAGACTCCAG TGCAGAGGAT CATACTTTGA 1681 GTGAACAGGA TTCCATACAA AAAAGTCCTT TATCAGAAAC TATGGATGTG AAGCCTTCTG 1741 ATATGACTTT ACCATTAGCT CTTCCACTTG GGACTGAGGA CCATCACATG CTTCTGCCTG 1801 TCACGGATAC TCAGTCTCCT ACATCAGATA CATTGTTGAG GTCAACTGTG AATGGGTATT 1861 CAGAACCACA GTTGATTTTT TTACAACAAT TATACTGCCC AACTTTCTTG TACAAAGTGG 1921 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1981 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2041 ACTTTCGTGT GTTCAGTATA AGCCTACGCG TTAAGTCgac aatcaacctc tggattacaa 2101 aatttgtgaa agatt