Construct: ORF TRCN0000474219

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017819.1_s317c1
Derived from:
ccsbBroadEn_04433
DNA Barcode:
CCAACCCAACTGCGAGTAGGCGGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LRCH3 (84859)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474219
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84859 LRCH3 leucine rich repeats and ca... NM_032773.3 99.9% 100% 1314T>C
2 human 84859 LRCH3 leucine rich repeats and ca... XM_017007350.2 92.5% 92.4% (many diffs)
3 human 84859 LRCH3 leucine rich repeats and ca... NM_001365715.1 91.5% 91.3% (many diffs)
4 human 84859 LRCH3 leucine rich repeats and ca... NM_001363887.1 88.5% 88.4% (many diffs)
5 human 84859 LRCH3 leucine rich repeats and ca... NM_001365717.1 88.4% 88.2% (many diffs)
6 human 84859 LRCH3 leucine rich repeats and ca... NM_001365718.1 86.8% 86.7% (many diffs)
7 human 84859 LRCH3 leucine rich repeats and ca... XM_005269363.3 85.5% 85.4% (many diffs)
8 human 84859 LRCH3 leucine rich repeats and ca... NM_001365716.1 84% 83.9% (many diffs)
9 human 84859 LRCH3 leucine rich repeats and ca... XM_017007351.2 83.3% 83.1% (many diffs)
10 human 84859 LRCH3 leucine rich repeats and ca... XM_005269367.3 81% 80.9% (many diffs)
11 human 84859 LRCH3 leucine rich repeats and ca... XM_011513242.3 80.9% 80.3% (many diffs)
12 human 84859 LRCH3 leucine rich repeats and ca... XM_017007352.2 78.8% 78.7% (many diffs)
13 human 84859 LRCH3 leucine rich repeats and ca... NM_001365719.2 77.2% 76.9% (many diffs)
14 human 84859 LRCH3 leucine rich repeats and ca... XR_001740329.2 48% (many diffs)
15 human 84859 LRCH3 leucine rich repeats and ca... NR_158582.1 19.5% (many diffs)
16 mouse 70144 Lrch3 leucine-rich repeats and ca... NM_001081255.1 77.4% 78.9% (many diffs)
17 mouse 70144 Lrch3 leucine-rich repeats and ca... NM_001310674.1 75.7% 77.2% (many diffs)
18 mouse 70144 Lrch3 leucine-rich repeats and ca... XM_006522524.3 75.7% 77.2% (many diffs)
19 mouse 70144 Lrch3 leucine-rich repeats and ca... XM_006522525.2 75.6% 77% (many diffs)
20 mouse 70144 Lrch3 leucine-rich repeats and ca... XM_006522526.3 73.8% 75% (many diffs)
21 mouse 70144 Lrch3 leucine-rich repeats and ca... NM_001310672.1 73.8% 74.7% (many diffs)
22 mouse 70144 Lrch3 leucine-rich repeats and ca... XM_006522527.3 73.7% 74.9% (many diffs)
23 mouse 70144 Lrch3 leucine-rich repeats and ca... XM_006522528.2 72.1% 73.1% (many diffs)
24 mouse 70144 Lrch3 leucine-rich repeats and ca... XM_006522530.2 70.1% 71.4% (many diffs)
25 mouse 70144 Lrch3 leucine-rich repeats and ca... XM_006522531.2 70% 71.3% (many diffs)
26 mouse 70144 Lrch3 leucine-rich repeats and ca... XM_006522533.3 68.2% 69.3% (many diffs)
27 mouse 70144 Lrch3 leucine-rich repeats and ca... NM_001310673.1 68% 68.9% (many diffs)
28 mouse 70144 Lrch3 leucine-rich repeats and ca... XR_001781866.1 21.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2202
ORF length:
2136
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggccgcgggc ttggtcgctg tggcagcggc tgccgagtac tctggcacgg
121tagcgtcggg aggtaacctc cctggtgttc actgcggccc aagctccggg gcaggccctg
181gttttggccc gggctcgtgg agccgctctc tcgatcgagc cctggaggag gcggcggtca
241ctggggtgct gagcctgagc ggccggaaac tgagggagtt tccccgggga gcggccaacc
301acgacctgac ggacaccacc cgggcggacc tgtcgcgaaa tcgcctttca gaaattccta
361tagaagcatg tcactttgtt tctctggaaa atctcaactt gtaccaaaat tgtattcgtt
421atattccaga ggcaatttta aacctacaag ctctaacatt cttaaatatt agtcggaacc
481aactgtcaac attgccggta cacttgtgta atttgccatt gaaagtctta attgctagta
541ataacaaatt ggtgtcactt ccagaagaaa ttggacacct tagacatttg atggaacttg
601atgtgagctg caatgaaatt caaactatac cttcccaaat tggtaacctg gaggccttga
661gagaccttaa tgtaagaaga aatcacctag tacatttgcc tgaagagctg gcggagttgc
721ctttgatacg gttagacttc tcatgcaata aaattaccac aatccctgtt tgttatcgga
781acctcaggca cctacagacg atcaccctag ataacaatcc actacaatca cctcctgcac
841agatatgtat aaaaggcaaa gtccacatat ttaaatacct gaacatacaa gcttgtaaga
901ttgctccaga tctgccggat tatgatagga gaccgttggg ttttggctcc tgccatgaag
961aactgtactc aagtcgccct tatggagccc ttgattcagg cttcaatagt gtggacagtg
1021gtgataagag atggtcaggg aatgaaccta cagatgaatt ttcagatctg cctcttcgag
1081tagcagagat tactaaagaa caaagactac gaagagaaag ccagtaccaa gagaaccgcg
1141gcagtttggt agtaacaaac ggcggagtgg aacatgatct ggatcagatt gactacatag
1201acagctgcac cgcagaggaa gaggaggccg aggtgagaca gcccaaggga ccagacccag
1261acagccttag ttcacagttt atggcgtata ttgaacagcg gcgaatctct catgagggtt
1321caccagtaaa gccagtagcc attagggagt ttcaaaaaac agaagatatg agaagatact
1381tacatcaaaa cagggttcca gctgagccat cttccctcct gtcactatca gcaagtcaca
1441atcagctgtc acacacagac ctggaacttc atcagagaag ggagcagtta gtagagcgca
1501ctcggagaga ggctcagctt gctgccctgc agtatgagga ggagaaaata aggaccaagc
1561agatccagag agatgctgtc ctggactttg tcaaacaaaa agcatcacaa agtccacaaa
1621aacagcaccc gctcctagat ggcgtagatg gtgagtgccc cttcccatcc agaaggtctc
1681agcacactga tgatagtgcc ttgtgcatgt cgctgtcagg gttgaatcaa gtgggctgtg
1741ctgctaccct gcctcattct tctgccttca cgcctcttaa gagtgatgac agacctaatg
1801ctctattaag ttcacctgca acagaaacag ttcatcattc ccctgcatat tcttttcctg
1861ctgctatcca gagaaatcag ccTCAGCGCC CTGAAAGCTT CCTTTTCCGA GCAGGTGTCA
1921GGGCAGAAAC CAACAAAGGT CATGCTTCAC CCCTTCCTCC ATCTGCTGCA CCTACCACTG
1981ATTCTACAGA TTCCATAACA GGACAGAATT CAAGACAGAG AGAAGAAGAG CTGGAATTAA
2041TAGACCAACT GCGTAAACAT ATTGAGTACC GGTTGAAAGT GTCTCTACCT TGTGATCTCG
2101GAGCAGCTCT AACTGACGGT GTTGTTCTTT GCCATTTGGC CAATCATGTG CGACCTCGAT
2161CTGTCCCAAG CATTCATGTT CCCTCACCAG CTGTAGTAAG TTGCCCAACT TTCTTGTACA
2221AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
2281AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
2341AGGACGACCA ACCCAACTGC GAGTAGGCGG AACGCGTTAA GTCgacaatc aacctctgga
2401ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)