Construct: ORF TRCN0000474312

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003928.1_s317c1
Derived from:
ccsbBroadEn_15614
DNA Barcode:
TCGGGCATGCTATAACTGGACCTG
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TPM3 (7170)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474312
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7170 TPM3 tropomyosin 3 NM_153649.4 99.8% 98.7% 736delA
2 human 7170 TPM3 tropomyosin 3 NM_001043351.2 94.9% 92.7% (many diffs)
3 human 7170 TPM3 tropomyosin 3 NM_001043352.2 94% 91.9% (many diffs)
4 human 7170 TPM3 tropomyosin 3 NM_001278189.2 92.4% 91.9% (many diffs)
5 human 7170 TPM3 tropomyosin 3 NM_001364683.1 92.4% 91.9% (many diffs)
6 human 7170 TPM3 tropomyosin 3 NM_001278190.1 91.3% 90.3% 529_530ins63;673delA
7 human 7170 TPM3 tropomyosin 3 NM_001043353.2 89.2% 85.9% (many diffs)
8 human 7170 TPM3 tropomyosin 3 NM_001349679.2 87.7% 85.8% (many diffs)
9 human 7170 TPM3 tropomyosin 3 NM_001364680.2 79.6% 73.8% (many diffs)
10 human 7170 TPM3 tropomyosin 3 NM_001364679.2 75.6% 68.7% (many diffs)
11 human 7170 TPM3 tropomyosin 3 NM_001364682.1 73.8% 68% (many diffs)
12 human 7170 TPM3 tropomyosin 3 NM_001278188.2 73.2% 65.7% 0_1ins64;66_67ins134;538delA
13 human 7170 TPM3 tropomyosin 3 NM_001364681.2 71% 63% (many diffs)
14 human 7170 TPM3 tropomyosin 3 NM_152263.4 69.9% 62.8% (many diffs)
15 human 7170 TPM3 tropomyosin 3 NM_001278191.2 58.9% 56.4% (many diffs)
16 human 147804 TPM3P9 tropomyosin 3 pseudogene 9 NR_003148.3 23.8% (many diffs)
17 human 7170 TPM3 tropomyosin 3 NR_103461.2 23.2% (many diffs)
18 mouse 59069 Tpm3 tropomyosin 3, gamma NM_001253738.1 93.1% 98.3% (many diffs)
19 mouse 59069 Tpm3 tropomyosin 3, gamma NM_001253740.1 88.5% 92.3% (many diffs)
20 mouse 59069 Tpm3 tropomyosin 3, gamma NM_001271764.1 86.1% 91.5% (many diffs)
21 mouse 59069 Tpm3 tropomyosin 3, gamma XM_017319677.1 86.1% 91.5% (many diffs)
22 mouse 59069 Tpm3 tropomyosin 3, gamma XM_006501839.2 84.3% 91.1% (many diffs)
23 mouse 59069 Tpm3 tropomyosin 3, gamma XM_006501835.2 84.2% 89.3% (many diffs)
24 mouse 59069 Tpm3 tropomyosin 3, gamma XM_006501838.1 82% 85.4% (many diffs)
25 mouse 59069 Tpm3 tropomyosin 3, gamma XM_017319678.1 81.8% 85.4% (many diffs)
26 mouse 59069 Tpm3 tropomyosin 3, gamma NM_001293749.1 80% 85.1% (many diffs)
27 mouse 59069 Tpm3 tropomyosin 3, gamma XM_006501836.2 79.9% 83.4% (many diffs)
28 mouse 59069 Tpm3 tropomyosin 3, gamma NM_022314.3 75.2% 74.9% (many diffs)
29 mouse 59069 Tpm3 tropomyosin 3, gamma XM_006501841.3 68.8% 64.9% (many diffs)
30 mouse 59069 Tpm3 tropomyosin 3, gamma XR_001783731.1 20.3% (many diffs)
31 mouse 59069 Tpm3 tropomyosin 3, gamma XR_001783732.1 19.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
810
ORF length:
744
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tgggatcacc accatcgagg cggtgaagcg caagatccag gttctgcagc
121agcaggcaga tgatgcagag gagcgagctg agcgcctcca gcgagaagtt gagggagaaa
181ggcgggcccg ggaacaggct gaggctgagg tggcctcctt gaaccgtagg atccagctgg
241ttgaagaaga gctggaccgt gctcaggagc gcctggccac tgccctgcaa aagctggaag
301aagctgaaaa agctgctgat gagagtgaga gaggtatgaa ggttattgaa aaccgggccT
361TAAAAGATGA AGAAAAGATG GAACTCCAGG AAATCCAACT CAAAGAAGCT AAGCACATTG
421CAGAAGAGGC AGATAGGAAG TATGAAGAGG TGGCTCGTAA GTTGGTGATC ATTGAAGGAG
481ACTTGGAACG CACAGAGGAA CGAGCTGAGC TGGCAGAGTC CCGTTGCCGA GAGATGGATG
541AGCAGATTAG ACTGATGGAC CAGAACCTGA AGTGTCTGAG TGCTGCTGAA GAAAAGTACT
601CTCAAAAAGA AGATAAATAT GAGGAAGAAA TCAAGATTCT TACTGATAAA CTCAAGGAGG
661CAGAGACCCG TGCTGAGTTT GCTGAGAGAT CGGTAGCCAA GCTGGAAAAG ACAATTGATG
721ACCTGGAAGA TAAACTGAAA TGCACCAAAG AGGAGCACCT CTGTACACAA AGGATGCTGG
781ACCAGACCCT GCTTGACCTG ATGAGATGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT
841CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC
901GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCGGGC
961ATGCTATAAC TGGACCTGAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt
1021gaaagatt

Download FASTA (ORF) (Full)

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