Construct: ORF TRCN0000474312
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003928.1_s317c1
- Derived from:
- ccsbBroadEn_15614
- DNA Barcode:
- TCGGGCATGCTATAACTGGACCTG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPM3 (7170)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474312
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7170 | TPM3 | tropomyosin 3 | NM_153649.4 | 99.8% | 98.7% | 736delA |
| 2 | human | 7170 | TPM3 | tropomyosin 3 | NM_001043351.2 | 94.9% | 92.7% | (many diffs) |
| 3 | human | 7170 | TPM3 | tropomyosin 3 | NM_001043352.2 | 94% | 91.9% | (many diffs) |
| 4 | human | 7170 | TPM3 | tropomyosin 3 | NM_001278189.2 | 92.4% | 91.9% | (many diffs) |
| 5 | human | 7170 | TPM3 | tropomyosin 3 | NM_001364683.1 | 92.4% | 91.9% | (many diffs) |
| 6 | human | 7170 | TPM3 | tropomyosin 3 | NM_001278190.1 | 91.3% | 90.3% | 529_530ins63;673delA |
| 7 | human | 7170 | TPM3 | tropomyosin 3 | NM_001043353.2 | 89.2% | 85.9% | (many diffs) |
| 8 | human | 7170 | TPM3 | tropomyosin 3 | NM_001349679.2 | 87.7% | 85.8% | (many diffs) |
| 9 | human | 7170 | TPM3 | tropomyosin 3 | NM_001364680.2 | 79.6% | 73.8% | (many diffs) |
| 10 | human | 7170 | TPM3 | tropomyosin 3 | NM_001364679.2 | 75.6% | 68.7% | (many diffs) |
| 11 | human | 7170 | TPM3 | tropomyosin 3 | NM_001364682.1 | 73.8% | 68% | (many diffs) |
| 12 | human | 7170 | TPM3 | tropomyosin 3 | NM_001278188.2 | 73.2% | 65.7% | 0_1ins64;66_67ins134;538delA |
| 13 | human | 7170 | TPM3 | tropomyosin 3 | NM_001364681.2 | 71% | 63% | (many diffs) |
| 14 | human | 7170 | TPM3 | tropomyosin 3 | NM_152263.4 | 69.9% | 62.8% | (many diffs) |
| 15 | human | 7170 | TPM3 | tropomyosin 3 | NM_001278191.2 | 58.9% | 56.4% | (many diffs) |
| 16 | human | 147804 | TPM3P9 | tropomyosin 3 pseudogene 9 | NR_003148.3 | 23.8% | (many diffs) | |
| 17 | human | 7170 | TPM3 | tropomyosin 3 | NR_103461.2 | 23.2% | (many diffs) | |
| 18 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_001253738.1 | 93.1% | 98.3% | (many diffs) |
| 19 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_001253740.1 | 88.5% | 92.3% | (many diffs) |
| 20 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_001271764.1 | 86.1% | 91.5% | (many diffs) |
| 21 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_017319677.1 | 86.1% | 91.5% | (many diffs) |
| 22 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_006501839.2 | 84.3% | 91.1% | (many diffs) |
| 23 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_006501835.2 | 84.2% | 89.3% | (many diffs) |
| 24 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_006501838.1 | 82% | 85.4% | (many diffs) |
| 25 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_017319678.1 | 81.8% | 85.4% | (many diffs) |
| 26 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_001293749.1 | 80% | 85.1% | (many diffs) |
| 27 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_006501836.2 | 79.9% | 83.4% | (many diffs) |
| 28 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_022314.3 | 75.2% | 74.9% | (many diffs) |
| 29 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_006501841.3 | 68.8% | 64.9% | (many diffs) |
| 30 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XR_001783731.1 | 20.3% | (many diffs) | |
| 31 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XR_001783732.1 | 19.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 810
- ORF length:
- 744
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgggatcacc accatcgagg cggtgaagcg caagatccag gttctgcagc 121 agcaggcaga tgatgcagag gagcgagctg agcgcctcca gcgagaagtt gagggagaaa 181 ggcgggcccg ggaacaggct gaggctgagg tggcctcctt gaaccgtagg atccagctgg 241 ttgaagaaga gctggaccgt gctcaggagc gcctggccac tgccctgcaa aagctggaag 301 aagctgaaaa agctgctgat gagagtgaga gaggtatgaa ggttattgaa aaccgggccT 361 TAAAAGATGA AGAAAAGATG GAACTCCAGG AAATCCAACT CAAAGAAGCT AAGCACATTG 421 CAGAAGAGGC AGATAGGAAG TATGAAGAGG TGGCTCGTAA GTTGGTGATC ATTGAAGGAG 481 ACTTGGAACG CACAGAGGAA CGAGCTGAGC TGGCAGAGTC CCGTTGCCGA GAGATGGATG 541 AGCAGATTAG ACTGATGGAC CAGAACCTGA AGTGTCTGAG TGCTGCTGAA GAAAAGTACT 601 CTCAAAAAGA AGATAAATAT GAGGAAGAAA TCAAGATTCT TACTGATAAA CTCAAGGAGG 661 CAGAGACCCG TGCTGAGTTT GCTGAGAGAT CGGTAGCCAA GCTGGAAAAG ACAATTGATG 721 ACCTGGAAGA TAAACTGAAA TGCACCAAAG AGGAGCACCT CTGTACACAA AGGATGCTGG 781 ACCAGACCCT GCTTGACCTG ATGAGATGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT 841 CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC 901 GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCGGGC 961 ATGCTATAAC TGGACCTGAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 1021 gaaagatt