Construct: ORF TRCN0000474481
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018925.3_s317c1
- Derived from:
- ccsbBroadEn_15247
- DNA Barcode:
- AATGAAAAATACAGCGTGCCCAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LRGUK (136332)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474481
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 136332 | LRGUK | leucine rich repeats and gu... | NM_144648.3 | 99.1% | 27.7% | (many diffs) |
| 2 | human | 136332 | LRGUK | leucine rich repeats and gu... | XM_024446661.1 | 94.3% | 29% | (many diffs) |
| 3 | human | 136332 | LRGUK | leucine rich repeats and gu... | XM_024446659.1 | 93.2% | 28.2% | (many diffs) |
| 4 | human | 136332 | LRGUK | leucine rich repeats and gu... | XM_024446660.1 | 93.2% | 28.2% | (many diffs) |
| 5 | human | 136332 | LRGUK | leucine rich repeats and gu... | XM_024446662.1 | 79.2% | 34.2% | (many diffs) |
| 6 | human | 136332 | LRGUK | leucine rich repeats and gu... | XR_002956408.1 | 76% | (many diffs) | |
| 7 | human | 136332 | LRGUK | leucine rich repeats and gu... | XR_002956409.1 | 74.7% | (many diffs) | |
| 8 | human | 136332 | LRGUK | leucine rich repeats and gu... | XM_024446663.1 | 74.1% | 36.8% | (many diffs) |
| 9 | human | 136332 | LRGUK | leucine rich repeats and gu... | XR_002956410.1 | 73.8% | (many diffs) | |
| 10 | human | 136332 | LRGUK | leucine rich repeats and gu... | NM_001365701.2 | 64.9% | 20.2% | (many diffs) |
| 11 | human | 136332 | LRGUK | leucine rich repeats and gu... | NM_001365700.2 | 64.8% | 20.2% | (many diffs) |
| 12 | human | 136332 | LRGUK | leucine rich repeats and gu... | XM_024446658.1 | 59.2% | 19.8% | (many diffs) |
| 13 | human | 136332 | LRGUK | leucine rich repeats and gu... | XM_017011749.1 | 50.3% | 5.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 765
- ORF length:
- 696
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgacctcc gagagggctc tcctgaggac cagagctgcc tctctcctga 121 gaggcttggg cagatcccga actggagccc gatcgttaca gtttcgcgca gaaaaagagc 181 gtcagccttg ctggtctttt cccatggggc agaagacgaa aggcagctct aacatagcct 241 cctcctacct gctccagcag ctcatgcacc gctatcagga gctggactcg gacggagatg 301 aggaccaggg cgagggcgag gcgggatccg aggagtcctc agagtccgaa atgctgaatt 361 tggaggagga atttgatggg gtcctgagag aggaggctgt ggccaaagca ctccatcact 421 tggggcgctc aggctctggg actgagcaag tctacctcaa tctaacttta tcaggttgta 481 atttaattga tgttagcatt ctctgtggat atgttcatct acagaagttg gatctttcag 541 cgaataaaat tgaagattta tcttgtgtga gttgtatgcc ttatctccta gaacttaatg 601 cttctcaaaa taatttgact acgttcttca atttcaagcc acccaaaaac ctcaagaaag 661 cggatttttc ccacaaccaa atttctgaaa tttgtgattt gtcagcgtat catgctctca 721 ctaaactaat tttggatggc aatgagatag aagaatcagt ggactagaga tgtgcacacc 781 taattcacct tagtttggcc acaatagatc acgacattaa tggcttaaac aagttaccaa 841 tcaaaatact ttgttctgag caataaccag attgagatga tcacaggttt ggaggatctg 901 aaagccctgc agaacctgga tctgtcccac aatcagataa gcagcctcca aggcttagag 961 aatcatgacc tcctggaagt gatcaacctg gaggataata agattgctga gctgagagaa 1021 atagaataca ttaaaaattt acccatcctt cgagttctca atcttctaga aaatccaatt 1081 caggaaaagt ctgaatattg gttcttcgta atttttatgc ttctgcgatt aacagaatta 1141 gatcagaaga agattaaagt ggaagaaaag gtttcagcag tgaataaata tgatcctccc 1201 cctgaagtgg ttgcagctca agaccacctg acccatgttg tcaacagcgt gatgcagccg 1261 cagaggatct ttgacagcac tcttcccagc ctggatgctc cttatcccat gctgatacta 1321 gctggtcctg aagcttgtgg gaaacgagag cttgcccatc gcctctgcag acagtttagc 1381 acttacttca gatatggggc ctgtcatacc acaagaccac cttactttgg agaaggggat 1441 cgagttgatt atcattttat ctctcaagac gtttttgatg aaatggtgaa catggggaaa 1501 ttcattctaa catttagtta tggtaatcac aagtatggat taaataggga caccgtagaa 1561 ggtatcgcaa gagatggttt ggcaagctgt attcatatgg aaatagaagg tgtaagaagt 1621 ttgaaatatt cctattttga gcctcgttat atcctggtgg tgcccatgaa caaggaaaaa 1681 tatgagggat atttgcggag aaaaggatta ttcagtcgtg cagaaattga atttgctgtc 1741 tccagagtgg acctttataa taaaaattaa tcagaatttt ccggggatat ttgaatgagg 1801 taatcaatgc agatgatctg gatgtgcctt acaaaaaact gagtcagctc attagagaat 1861 accttggatg gaatgaggaa cctgccaaga gtttggctac aactgcagat gtaaagacat 1921 cccacctgaa acctgaagcg catcctacaa agtatatttc ttcgaatatg ggtgatttcc 1981 tgcattctac agacagaaac tacctcatta aattttgggc caaactttca gccaaaaaaa 2041 caccagcgga aagagattct atacacagac agcacgaggc agcccggcaa gctctaatgg 2101 gaaggatacg ccctgatcac acactcctat ttcaaagggg tccagtaCCA GCACCTCTCA 2161 CCAGTGGTCT ACACTATTAT ACAACTTTAG AAGAACTCTG GAAAAGTTTT GATCTTTGTG 2221 AAGACTATTT TAAACCTCCA TTTGGACCAT ATCCTGAAAA GAGTGGGAAG GATTCCTTGG 2281 TTTCCATGAA ATGTTCATTG TTTCGGTTCT GTCCGTGGTC AAAAGAATTG CCTTTCCAGC 2341 CTCCGGAGGG GAGCATTTCT TCACACCTAG GATCAGGAGC CAGTGACAGT GAGACCGAAG 2401 AGACCCGGAA AGCACTACCT ATACAATCAT TTTCACATGA AAAAGAGTCT CACCAACACA 2461 GACAACACTC GGTCCCAGTC ATCAGTCGCC CAGGTTCCAA CGTCAAACCC ACCCTCCCTC 2521 CAATCCCTCA GGGCCGCAGG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2581 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAGCTTG 2641 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAATG AAAAATACAG 2701 CGTGCCCAAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt