Transcript: Human XM_011517583.3

PREDICTED: Homo sapiens ubiquitin conjugating enzyme E2 V2 (UBE2V2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
UBE2V2 (7336)
Length:
3251
CDS:
16..537

Additional Resources:

NCBI RefSeq record:
XM_011517583.3
NBCI Gene record:
UBE2V2 (7336)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011517583.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000004037 GCTCCTCCGTCAGTTAGATTT pLKO.1 325 CDS 100% 13.200 18.480 N UBE2V2 n/a
2 TRCN0000273329 GCTCCTCCGTCAGTTAGATTT pLKO_005 325 CDS 100% 13.200 18.480 N UBE2V2 n/a
3 TRCN0000284952 TCAAGAGCTAAGACGTCTAAT pLKO_005 456 CDS 100% 13.200 18.480 N UBE2V2 n/a
4 TRCN0000010843 GCCCGGAGCATACCAGTGTTA pLKO.1 397 CDS 100% 1.650 2.310 N UBE2V2 n/a
5 TRCN0000004036 GTCTTAAATCAACAACCTTCT pLKO.1 558 3UTR 100% 4.050 3.240 N UBE2V2 n/a
6 TRCN0000273328 GTCTTAAATCAACAACCTTCT pLKO_005 558 3UTR 100% 4.050 3.240 N UBE2V2 n/a
7 TRCN0000284956 GGGCCACCAAGGACAAATTAT pLKO_005 253 CDS 100% 15.000 10.500 N UBE2V2 n/a
8 TRCN0000004034 CAAGGTGGACAGGCATGATTA pLKO.1 230 CDS 100% 13.200 9.240 N UBE2V2 n/a
9 TRCN0000273330 CAAGGTGGACAGGCATGATTA pLKO_005 230 CDS 100% 13.200 9.240 N UBE2V2 n/a
10 TRCN0000004035 CAGAAGGACAAACATACAACA pLKO.1 512 CDS 100% 4.950 3.465 N UBE2V2 n/a
11 TRCN0000040916 CGCTTGTTGGAAGAACTTGAA pLKO.1 139 CDS 100% 4.950 3.465 N Ube2v2 n/a
12 TRCN0000349339 CGCTTGTTGGAAGAACTTGAA pLKO_005 139 CDS 100% 4.950 3.465 N Ube2v2 n/a
13 TRCN0000040917 GCATGATTATTGGGCCACCAA pLKO.1 242 CDS 100% 2.640 1.848 N Ube2v2 n/a
14 TRCN0000040915 GCCTGAAAGTAGAATGTGGAT pLKO.1 290 CDS 100% 2.640 1.848 N Ube2v2 n/a
15 TRCN0000172586 GCCTGGCCAACATGATGAAAT pLKO.1 2106 3UTR 100% 13.200 6.600 Y SPIRE2 n/a
16 TRCN0000162166 CAACATGATGAAACCCTGTAT pLKO.1 2113 3UTR 100% 4.950 2.475 Y SWSAP1 n/a
17 TRCN0000164260 CCAACATGATGAAACCCTGTA pLKO.1 2112 3UTR 100% 4.050 2.025 Y SWSAP1 n/a
18 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 2077 3UTR 100% 4.050 2.025 Y P3H4 n/a
19 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 2077 3UTR 100% 4.050 2.025 Y ORAI2 n/a
20 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 2077 3UTR 100% 4.050 2.025 Y P3H4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011517583.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01742 pDONR223 100% 83.4% 80.9% None (many diffs) n/a
2 ccsbBroad304_01742 pLX_304 0% 83.4% 80.9% V5 (many diffs) n/a
3 TRCN0000474497 GGCACAGCCACCCTAATTTTCGTC pLX_317 87.6% 83.4% 80.9% V5 (many diffs) n/a
4 ccsbBroadEn_01741 pDONR223 100% 71.4% 73.9% None (many diffs) n/a
5 ccsbBroad304_01741 pLX_304 0% 71.4% 73.9% V5 (many diffs) n/a
6 TRCN0000468843 CTGAGCTAAAGGCTACTCTTGCGC pLX_317 82.6% 71.4% 73.9% V5 (many diffs) n/a
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