Construct: ORF TRCN0000474694

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010373.1_s317c1
Derived from:
ccsbBroadEn_03798
DNA Barcode:
TTCCGATACCCTGCCCGTAGGATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PHTF2 (57157)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474694
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57157 PHTF2 putative homeodomain transc... NM_001127357.2 100% 100%
2 human 57157 PHTF2 putative homeodomain transc... XM_005250508.5 100% 100%
3 human 57157 PHTF2 putative homeodomain transc... XM_005250509.4 100% 100%
4 human 57157 PHTF2 putative homeodomain transc... NM_001127358.2 99.4% 99.4% 101_102insGTTTATTAAACT
5 human 57157 PHTF2 putative homeodomain transc... NM_001366086.1 99.4% 99.4% 101_102insGTTTATTAAACT
6 human 57157 PHTF2 putative homeodomain transc... NM_020432.5 99.4% 99.4% 101_102insGTTTATTAAACT
7 human 57157 PHTF2 putative homeodomain transc... NM_001366089.1 95.6% 95.6% 45_146del
8 human 57157 PHTF2 putative homeodomain transc... XM_006716063.4 94.2% 94.2% 1236_1237ins129
9 human 57157 PHTF2 putative homeodomain transc... XM_011516422.3 94.1% 94.1% 1636_1637ins132
10 human 57157 PHTF2 putative homeodomain transc... NM_001366084.1 93.6% 93.6% 101_102insGTTTATTAAACT;1624_1625ins132
11 human 57157 PHTF2 putative homeodomain transc... NM_001366087.1 93.6% 93.6% 101_102insGTTTATTAAACT;1624_1625ins132
12 human 57157 PHTF2 putative homeodomain transc... NM_001366081.1 92.5% 92.5% 101_102insGTTTATTAAACT;1842_1843ins156
13 human 57157 PHTF2 putative homeodomain transc... XM_011516423.3 90.2% 90.2% 1015_1016ins219
14 human 57157 PHTF2 putative homeodomain transc... XM_017012459.2 89.7% 89.7% 101_102insGTTTATTAAACT;1003_1004ins219
15 human 57157 PHTF2 putative homeodomain transc... XM_011516424.2 81.2% 81.2% 0_1ins423
16 human 57157 PHTF2 putative homeodomain transc... NM_001366082.2 72.2% 72.1% (many diffs)
17 human 57157 PHTF2 putative homeodomain transc... NM_001366088.2 72.2% 72.1% (many diffs)
18 human 57157 PHTF2 putative homeodomain transc... NM_001366085.2 62.5% 62.4% (many diffs)
19 human 57157 PHTF2 putative homeodomain transc... NM_001366083.1 60.4% 60.1% (many diffs)
20 human 57157 PHTF2 putative homeodomain transc... NM_001127359.1 46.7% 44.9% (many diffs)
21 human 57157 PHTF2 putative homeodomain transc... NM_001127360.1 46.2% 44.4% (many diffs)
22 mouse 68770 Phtf2 putative homeodomain transc... NM_172992.3 88.6% 93.3% (many diffs)
23 mouse 68770 Phtf2 putative homeodomain transc... XM_006535801.1 79.7% 84.2% (many diffs)
24 mouse 68770 Phtf2 putative homeodomain transc... XM_006535803.1 52.4% 51% (many diffs)
25 mouse 68770 Phtf2 putative homeodomain transc... XM_006535804.3 40.2% 40% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2322
ORF length:
2253
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcgtccaaa gtcacagatg ctatagtctg gtatcaaaag aagattggag
121catatgatca acaaatatgg gaaaaatctg ttgaacagag agaaatcaag tttattaaac
181tggggctaag gaataaacca aagaaaacag cacatgtgaa accagacctc atagatgttg
241atcttgtaag agggtctgca tttgcaaagg caaagcctga aagtccttgg acttctctga
301ccagaaaggg aattgttcga gttgtatttt tccccttttt cttccggtgg tggttacaag
361taacatcaaa ggtcatcttt ttctggcttc ttgtccttta tcttcttcaa gttgctgcaa
421tagtattatt ctgctccact tctagcccac acagcatacc tctgacagag gtgattgggc
481cgatatggct gatgctgctc ctgggaactg tgcattgcca gattgtttcc acaagaacac
541ccaaacctcc tctaagtaca gggggtaaaa gaagaaggaa attaagaaaa gcagcccatt
601tggaagtaca tagggaagga gatggttcta gtaccacaga taacacacaa gagggagcag
661ttcagaacca cggtacaagc acctctcaca gcgttggcac tgtcttcaga gatctctggc
721atgctgcttt ctttttatca ggatcaaaga aagcaaagaa ttcaattgat aaatcaactg
781aaactgacaa tggctatgta tcccttgatg ggaagaagac tgttaaaagc ggtgaagatg
841gaatacaaaa ccatgaacct cagtgtgaaa ctattcgacc agaagagaca gcctggaaca
901caggaacact gaggaatggt cctagcaaag atacccaaag gacaataaca aatgtctctg
961atgaagtctc cagtgaggaa ggtcctgaaa caggatactc attacgtcgt catgtggaca
1021ggacttctga aggtgttctt cggaatagaa agtcacacca ttataagaaa cattacccta
1081atgaggacgc ccctaaatcg ggtactagtt gcagctctcg ctgttcaagt tccagacagg
1141attctgagag tgcaaggcca gaatctgaaa cagaagatgt gttatgggaa gacttgttac
1201attgtgcaga atgccattca tcttgtacca gtgagacaga tgtggaaaat catcagatta
1261atccatgtgt gaaaaaagaa tatagagatg acccttttca tcagagtcat ttgccctggc
1321tccatagttc ccacccagga ttagaaaaaa taagtgctat agtatgggaa ggtaatgatt
1381gtaagaaagc agacatgtct gtacttgaaa tcagtggaat gataatgaac agagtgaaca
1441gccatatacc aggaatagga taccagattt ttggaaatgc agtctctctc atactgggtt
1501taactccatt tgttttccga ctttctcaag ctacagactt ggaacaactc acagcacatt
1561ctgcttcaga actttatgtg attgcatttg gttctaatga agatgtcata gttctttcta
1621tggttataat aagttttgtg gttcgcgtgt ctcttgtgtg gattttcttt tttttgctct
1681gtgtagcaga aagaacttat aaacagcgat tactttttgc aaaactcttt ggacatttaa
1741catctgcaag gagggctcga aaatctgagg ttcctcattt ccggttgaag aaagtacaga
1801atataaaaat gtggctatct ctccgttcct atcttaagcg tcgaggtcct cagcgatcag
1861ttgatgtaat agtttcaTCT GCTTTCTTAT TGACTATCTC AGTTGTATTT ATCTGTTGTG
1921CCCAGCTACT TCATGTACAC GAGATCTTCC TTGATTGTCA CTACAATTGG GAATTGGTAA
1981TCTGGTGCAT CTCGTTAACA CTTTTTCTCC TAAGATTTGT TACCCTTGGA TCAGAAACAA
2041GTAAAAAATA TAGTAATACC TCAATATTAC TTACTGAACA GATAAACCTC TACTTGAAAA
2101TGGAGAAAAA ACCTAACAAA AAGGAGGAAC TGACACTAGT GAATAATGTT TTAAAACTGG
2161CTACTAAACT GCTAAAGGAG TTGGACAGTC CTTTTAGATT ATATGGGCTT ACAATGAATC
2221CGCTGCTTTA TAACATCACC CAGGTTGTTA TCCTGTCAGC TGTTTCTGGT GTTATCAGTG
2281ACTTGCTTGG ATTTAATTTA AAGCTATGGA AGATTAAGTC ATTGCCAACT TTCTTGTACA
2341AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
2401AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
2461AGGACGATTC CGATACCCTG CCCGTAGGAT CACGCGTTAA GTCgacaatc aacctctgga
2521ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)