Construct: ORF TRCN0000474895
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006428.2_s317c1
- Derived from:
- ccsbBroadEn_14656
- DNA Barcode:
- TTTCTTGTATCACAAGGATTACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GK2 (2712)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474895
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2712 | GK2 | glycerol kinase 2 | NM_033214.3 | 99.9% | 100% | 12A>G |
| 2 | human | 2710 | GK | glycerol kinase | NM_001128127.2 | 88.1% | 88.4% | (many diffs) |
| 3 | human | 2710 | GK | glycerol kinase | NM_001205019.1 | 87.2% | 87.4% | (many diffs) |
| 4 | human | 2710 | GK | glycerol kinase | XM_006724483.2 | 83.8% | 84.1% | (many diffs) |
| 5 | human | 2710 | GK | glycerol kinase | NM_000167.5 | 83.4% | 83.3% | (many diffs) |
| 6 | human | 2710 | GK | glycerol kinase | XM_011545491.2 | 83% | 83.3% | (many diffs) |
| 7 | human | 2710 | GK | glycerol kinase | NM_203391.3 | 82.6% | 82.4% | (many diffs) |
| 8 | human | 2710 | GK | glycerol kinase | XM_006724484.2 | 79.4% | 79.3% | (many diffs) |
| 9 | human | 2710 | GK | glycerol kinase | XM_011545492.1 | 78.7% | 78.5% | (many diffs) |
| 10 | human | 2713 | GK3P | glycerol kinase 3 pseudogene | NR_026575.1 | 64.9% | (many diffs) | |
| 11 | human | 2710 | GK | glycerol kinase | XM_005274488.4 | 55% | 55.8% | (many diffs) |
| 12 | human | 2710 | GK | glycerol kinase | XM_006724485.2 | 54.5% | 55.2% | (many diffs) |
| 13 | human | 2710 | GK | glycerol kinase | XM_006724486.3 | 54.5% | 55.2% | (many diffs) |
| 14 | human | 2710 | GK | glycerol kinase | XM_011545493.2 | 54.5% | 55.2% | (many diffs) |
| 15 | human | 2710 | GK | glycerol kinase | XM_011545494.2 | 54.5% | 55.2% | (many diffs) |
| 16 | human | 2710 | GK | glycerol kinase | XM_017029409.1 | 54.5% | 55.2% | (many diffs) |
| 17 | human | 2710 | GK | glycerol kinase | XM_017029411.1 | 50.3% | 50.8% | (many diffs) |
| 18 | human | 2710 | GK | glycerol kinase | XM_017029412.2 | 50.3% | 50.8% | (many diffs) |
| 19 | human | 2710 | GK | glycerol kinase | XM_017029410.1 | 49.9% | 50.2% | (many diffs) |
| 20 | mouse | 14933 | Gk | glycerol kinase | NM_212444.2 | 86.9% | 87.8% | (many diffs) |
| 21 | mouse | 14933 | Gk | glycerol kinase | NM_001294140.1 | 86% | 86.9% | (many diffs) |
| 22 | mouse | 14933 | Gk | glycerol kinase | XM_006527831.3 | 82.7% | 83.6% | (many diffs) |
| 23 | mouse | 14933 | Gk | glycerol kinase | NM_008194.3 | 82.3% | 82.8% | (many diffs) |
| 24 | mouse | 14933 | Gk | glycerol kinase | XM_006527829.3 | 81.9% | 82.7% | (many diffs) |
| 25 | mouse | 14933 | Gk | glycerol kinase | NM_001331046.1 | 81.4% | 81.9% | (many diffs) |
| 26 | mouse | 14933 | Gk | glycerol kinase | NM_001294138.1 | 78.3% | 78.8% | (many diffs) |
| 27 | mouse | 14933 | Gk | glycerol kinase | XM_006527833.3 | 77.6% | 78% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1725
- ORF length:
- 1659
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agccccgaag acagcagctg tggggccgtt ggtgggagcg gtggtccagg 121 gcaccaactc cactcgcttt ctggttttca attcaaaaac agcggaacta cttagtcatc 181 acaaagtgga attaacacaa gagttcccaa aagaaggatg ggtggaacaa gaccctaaag 241 aaattcttca gtctgtctac gagtgtatag cgagaacgtg tgagaaactt gacgaactga 301 atattgatat atccaacata aaagctgttg gtgtcagcaa tcagagggaa accactgtaa 361 tctgggacaa gttaacagga gagcctctct acaatgctgt ggtgtggctt gatctaagaa 421 cccagactac tgttgaggat cttagtaaaa aaattccagg aaatagtaac ttcgtcaagt 481 ctaagacagg ccttccactc agcacttact tcagtgcagt aaaacttcgt tggatgcttg 541 acaatgtgag aaacgtccaa aaggctgttg aagaaggtag agctcttttt ggtaccattg 601 attcatggct tatctggagt ttgacaggag gagttaatgg aggcgtgcat tgtacagatg 661 taacaaatgc aagtaggaca atgcttttta atatccattc tttggaatgg gataaagagc 721 tctgtgactt ttttgaaatt ccaatggacc ttcttccaaa tgtcttcagt tcttctgaga 781 tctatggcct aattaaaact ggagccctgg aaggtgtgcc aatatctggg tgtttggggg 841 accaatgtgc tgcattagta ggacaaatgt gcttccagga gggacaagcc aaaaacacct 901 atggaacagg ttgcttctta ctgtgtaata cgggtcgtaa atgtgtgttt tctgaacatg 961 gccttttgac cacagtagct tacaaactag gcagagagaa gccagcatat tatgcactgg 1021 aaggttctgt tgctatagca ggtgctgtta ttcgttggct aagagacaat cttggaatta 1081 tagagacctc aggagacatt gaaagacttg ctaaagaagt aggaacttct tatggctgtt 1141 actttgtccc agccttttca gggttatatg caccttattg ggagcccagt gcaagaggga 1201 tactctgtgg CCTCACTCAG TTTACCAATA AATGTCATAT TGCTTTTGCT GCATTAGAAG 1261 CTGTTTGTTT CCAAACCCGA GAGATTTTGG AAGCCATGAA CCGTGACTGT GGAATTCCAC 1321 TTCGTCATTT GCAGGTAGAT GGAGGAATGA CCAACAACAA AGTTCTTATG CAGCTACAAG 1381 CAGATATTCT TCATATTCCA GTAATAAAAC CCTTTATGCC TGAAACAACT GCACTAGGAG 1441 CTGCCATGGC AGCAGGGGCT GCAGAGGGAG TAAGCGTTTG GAGCCTTGAA CCCCAGGCTT 1501 TGTCAGTTCT CAGGATGGAA CGATTTGAAC CACAGATCCA GGCCACAGAA AGTGAAATTC 1561 GTTATGCCAC ATGGAAGAAA GCCGTAATGA AGTCAATGGG TTGGGTTACC AGTCAGTCTC 1621 CTGAAGGTGG TGATCCTTCT ATCTTCTCTA GTCTGCCTTT GGGATTTTTT ATAGTGAGTA 1681 GCATGGTAAT GCTAATTGGA GCAAGATATA TCTCGGGTGT GCCATACCCA ACTTTCTTGT 1741 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1801 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1861 GAAAGGACGA TTTCTTGTAT CACAAGGATT ACCAACGCGT TAAGTCgaca atcaacctct 1921 ggattacaaa atttgtgaaa gatt