Construct: ORF TRCN0000475094

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017520.1_s317c1
Derived from:
ccsbBroadEn_13499
DNA Barcode:
TCTTCTCCGAATTGTACACATATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KSR2 (283455)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475094
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 283455 KSR2 kinase suppressor of ras 2 XM_011538225.3 99.9% 100% 153G>C;858C>T
2 human 283455 KSR2 kinase suppressor of ras 2 NM_173598.6 87.1% 87.2% 1_363del;516G>C;1221C>T
3 human 283455 KSR2 kinase suppressor of ras 2 XM_011538224.3 86.9% 87% (many diffs)
4 human 283455 KSR2 kinase suppressor of ras 2 XM_017019208.2 83.9% 81.9% (many diffs)
5 human 283455 KSR2 kinase suppressor of ras 2 XM_011538226.3 65.1% 65% (many diffs)
6 human 283455 KSR2 kinase suppressor of ras 2 XM_017019210.2 61.8% 61.5% (many diffs)
7 human 283455 KSR2 kinase suppressor of ras 2 XM_011538229.3 52.2% 52.2% (many diffs)
8 human 283455 KSR2 kinase suppressor of ras 2 XM_017019209.2 51.6% 50.8% (many diffs)
9 mouse 333050 Ksr2 kinase suppressor of ras 2 NM_001312914.1 77.5% 83.9% (many diffs)
10 mouse 333050 Ksr2 kinase suppressor of ras 2 XM_006530393.3 77.3% 83.7% (many diffs)
11 mouse 333050 Ksr2 kinase suppressor of ras 2 XM_006530394.3 69.6% 74.7% (many diffs)
12 mouse 333050 Ksr2 kinase suppressor of ras 2 XM_006530395.3 61.9% 66.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2556
ORF length:
2487
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gacggatgaa caggtgtgcg agactgtgga gaaatacgga gccaaccggg
121aggagtgtgc ccgcctcaac gcctccctct cctgcctcag gaatgtccac atgtcaggag
181gcaacctttc caaacaagac tggaccatcc agtggcccac cacagagacg gggaaggaga
241acaatcccgt gtgccccccg gagcccaccc cgtggatccg cacccatctc tcccagagcc
301ccagggtccc gtccaagtgc gtccagcact attgtcacac cagccccact cccggggccc
361ctgtgtacac ccacgtggac aggcttaccg tggacgccta cccgggcttg tgcccgcccc
421cgccactgga gtcgggccac cgttccctgc ccccatcgcc ccggcagcgg cacgcggtcc
481gcaccccgcc gcgcaccccc aacatcgtca ccaccgtgac cccgccgggc acgccgccca
541tgaggaagaa gaacaagctg aagcccccgg ggaccccacc gccctcctcc cgaaaactga
601tacacttgat cccgggattc accgcgctgc atcggagcaa atcccacgag ttccagctgg
661ggcaccgcgt ggacgaggcc cacacgccca aagccaagaa gaagagcaaa cccttgaacc
721tcaagatcca cagcagcgta ggcagctgcg agaacatccc ctctcagcag cgctccccgc
781tgctgtccga gcgctccctc cgctccttct ttgtgggaca cgcacctttc ctgccttcca
841cccctcctgt tcacactgag gccaacttct ctgcaaacac actgtcagtg ccacgctggt
901ccccgcagat ccctcgcaga gatcttggca actccatcaa gcacaggttt tccaccaagt
961actggatgtc tcagacgtgc acagtctgtg ggaaagggat gctttttggc ctcaagtgta
1021aaaactgcaa gttaaagtgc cacaacaaat gcaccaaaga agccccaccc tgtcatcttc
1081tgatcatcca ccgaggagat ccagcaaggt tagtccggac agagtccgtt ccgtgtgaca
1141tcaacaaccc tctacggaag ccacctcgct attcagacct gcacatcagt cagacgctcc
1201ccaaaaccaa caaaatcaac aaggaccaca tccctgtccc ttaccagcca gactccagca
1261gcaacccctc ctccacgacg tcctccacgc cctcctcgcc agcacccccc ctccctccta
1321gtgccacgcc gccttctccc ctacaccctt ccccacagtg cacacggcag cagaagaact
1381tcaacctgcc agcatcccac tactacaaat acaagcagca gttcatcttc ccagatgtgg
1441tgccggtgcc ggagacgccg acccgggcgc cccaggtcat cctgcatccg gtgacctcga
1501atccaatctt ggaaggaaat ccattacttc aaattgaagt ggagccaacg tcggagaatg
1561aagaggtcca tgatgaggcc gaagagtcag aggatgactt cgaggagatg aacctgtccc
1621tcctctcggc ccggagcttc ccacgcaagg ccagccagac cagcatcttc cttcaggagt
1681gggacatccc ctttgagcag ctggagatcg gcgagctcat tggaaagggc cgctttgggc
1741aagtgtacca cggccgctgg catggcgagg tggccatccg gctgattgac attgagaggg
1801acaacgagga ccagctcaag gccttcaagc gggaggtgat ggcctacagg cagacacggc
1861atgagaacgt ggtgcttttc atgggtgcct gcatgagccc gcctcacctg gccatcatca
1921ccagcctctg taagggacgg acgctctatt ccgttgtgag ggatgccaaa atcgttttgg
1981atgtcaacaa aaccaggcag attgctcaag aaattgtgaa gggcatgggc tacctccacg
2041ccaagggaat cctacacaag gacctcaagt caaagaacgt cttctatgac aacggcaaag
2101tggtcatcac ggactttgga ctcttcagca tttctggggt gctgcaggct ggcaggcggg
2161aggacaaact gcgcatccag aatggctggc TATGCCACCT GGCACCAGAG ATCATCCGCC
2221AGCTGTCCCC CGACACAGAG GAGGATAAGC TCCCCTTCTC CAAGCACTCT GACGTCTTTG
2281CCCTTGGCAC AATCTGGTAT GAACTCCACG CCAGGGAATG GCCTTTCAAG ACCCAACCAG
2341CAGAGGCAAT AATCTGGCAA ATGGGCACAG GCATGAAACC CAACCTCAGC CAGATTGGCA
2401TGGGAAAAGA AATCTCGGAC ATTCTTCTCT TCTGCTGGGC CTTTGAACAA GAAGAGAGAC
2461CTACCTTCAC CAAGCTCATG GACATGCTGG AGAAACTGCC AAAGCGAAAC CGTCGCCTGT
2521CTCACCCTGG ACATTTCTGG AAGTCTGCAG AGCTGTTGCC AACTTTCTTG TACAAAGTGG
2581TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
2641CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
2701ATCTTCTCCG AATTGTACAC ATATCACGCG TTAAGTCgac aatcaacctc tggattacaa
2761aatttgtgaa agatt

Download FASTA (ORF) (Full)