Construct: ORF TRCN0000475094
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017520.1_s317c1
- Derived from:
- ccsbBroadEn_13499
- DNA Barcode:
- TCTTCTCCGAATTGTACACATATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KSR2 (283455)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475094
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_011538225.3 | 99.9% | 100% | 153G>C;858C>T |
| 2 | human | 283455 | KSR2 | kinase suppressor of ras 2 | NM_173598.6 | 87.1% | 87.2% | 1_363del;516G>C;1221C>T |
| 3 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_011538224.3 | 86.9% | 87% | (many diffs) |
| 4 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_017019208.2 | 83.9% | 81.9% | (many diffs) |
| 5 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_011538226.3 | 65.1% | 65% | (many diffs) |
| 6 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_017019210.2 | 61.8% | 61.5% | (many diffs) |
| 7 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_011538229.3 | 52.2% | 52.2% | (many diffs) |
| 8 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_017019209.2 | 51.6% | 50.8% | (many diffs) |
| 9 | mouse | 333050 | Ksr2 | kinase suppressor of ras 2 | NM_001312914.1 | 77.5% | 83.9% | (many diffs) |
| 10 | mouse | 333050 | Ksr2 | kinase suppressor of ras 2 | XM_006530393.3 | 77.3% | 83.7% | (many diffs) |
| 11 | mouse | 333050 | Ksr2 | kinase suppressor of ras 2 | XM_006530394.3 | 69.6% | 74.7% | (many diffs) |
| 12 | mouse | 333050 | Ksr2 | kinase suppressor of ras 2 | XM_006530395.3 | 61.9% | 66.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2556
- ORF length:
- 2487
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacggatgaa caggtgtgcg agactgtgga gaaatacgga gccaaccggg 121 aggagtgtgc ccgcctcaac gcctccctct cctgcctcag gaatgtccac atgtcaggag 181 gcaacctttc caaacaagac tggaccatcc agtggcccac cacagagacg gggaaggaga 241 acaatcccgt gtgccccccg gagcccaccc cgtggatccg cacccatctc tcccagagcc 301 ccagggtccc gtccaagtgc gtccagcact attgtcacac cagccccact cccggggccc 361 ctgtgtacac ccacgtggac aggcttaccg tggacgccta cccgggcttg tgcccgcccc 421 cgccactgga gtcgggccac cgttccctgc ccccatcgcc ccggcagcgg cacgcggtcc 481 gcaccccgcc gcgcaccccc aacatcgtca ccaccgtgac cccgccgggc acgccgccca 541 tgaggaagaa gaacaagctg aagcccccgg ggaccccacc gccctcctcc cgaaaactga 601 tacacttgat cccgggattc accgcgctgc atcggagcaa atcccacgag ttccagctgg 661 ggcaccgcgt ggacgaggcc cacacgccca aagccaagaa gaagagcaaa cccttgaacc 721 tcaagatcca cagcagcgta ggcagctgcg agaacatccc ctctcagcag cgctccccgc 781 tgctgtccga gcgctccctc cgctccttct ttgtgggaca cgcacctttc ctgccttcca 841 cccctcctgt tcacactgag gccaacttct ctgcaaacac actgtcagtg ccacgctggt 901 ccccgcagat ccctcgcaga gatcttggca actccatcaa gcacaggttt tccaccaagt 961 actggatgtc tcagacgtgc acagtctgtg ggaaagggat gctttttggc ctcaagtgta 1021 aaaactgcaa gttaaagtgc cacaacaaat gcaccaaaga agccccaccc tgtcatcttc 1081 tgatcatcca ccgaggagat ccagcaaggt tagtccggac agagtccgtt ccgtgtgaca 1141 tcaacaaccc tctacggaag ccacctcgct attcagacct gcacatcagt cagacgctcc 1201 ccaaaaccaa caaaatcaac aaggaccaca tccctgtccc ttaccagcca gactccagca 1261 gcaacccctc ctccacgacg tcctccacgc cctcctcgcc agcacccccc ctccctccta 1321 gtgccacgcc gccttctccc ctacaccctt ccccacagtg cacacggcag cagaagaact 1381 tcaacctgcc agcatcccac tactacaaat acaagcagca gttcatcttc ccagatgtgg 1441 tgccggtgcc ggagacgccg acccgggcgc cccaggtcat cctgcatccg gtgacctcga 1501 atccaatctt ggaaggaaat ccattacttc aaattgaagt ggagccaacg tcggagaatg 1561 aagaggtcca tgatgaggcc gaagagtcag aggatgactt cgaggagatg aacctgtccc 1621 tcctctcggc ccggagcttc ccacgcaagg ccagccagac cagcatcttc cttcaggagt 1681 gggacatccc ctttgagcag ctggagatcg gcgagctcat tggaaagggc cgctttgggc 1741 aagtgtacca cggccgctgg catggcgagg tggccatccg gctgattgac attgagaggg 1801 acaacgagga ccagctcaag gccttcaagc gggaggtgat ggcctacagg cagacacggc 1861 atgagaacgt ggtgcttttc atgggtgcct gcatgagccc gcctcacctg gccatcatca 1921 ccagcctctg taagggacgg acgctctatt ccgttgtgag ggatgccaaa atcgttttgg 1981 atgtcaacaa aaccaggcag attgctcaag aaattgtgaa gggcatgggc tacctccacg 2041 ccaagggaat cctacacaag gacctcaagt caaagaacgt cttctatgac aacggcaaag 2101 tggtcatcac ggactttgga ctcttcagca tttctggggt gctgcaggct ggcaggcggg 2161 aggacaaact gcgcatccag aatggctggc TATGCCACCT GGCACCAGAG ATCATCCGCC 2221 AGCTGTCCCC CGACACAGAG GAGGATAAGC TCCCCTTCTC CAAGCACTCT GACGTCTTTG 2281 CCCTTGGCAC AATCTGGTAT GAACTCCACG CCAGGGAATG GCCTTTCAAG ACCCAACCAG 2341 CAGAGGCAAT AATCTGGCAA ATGGGCACAG GCATGAAACC CAACCTCAGC CAGATTGGCA 2401 TGGGAAAAGA AATCTCGGAC ATTCTTCTCT TCTGCTGGGC CTTTGAACAA GAAGAGAGAC 2461 CTACCTTCAC CAAGCTCATG GACATGCTGG AGAAACTGCC AAAGCGAAAC CGTCGCCTGT 2521 CTCACCCTGG ACATTTCTGG AAGTCTGCAG AGCTGTTGCC AACTTTCTTG TACAAAGTGG 2581 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2641 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2701 ATCTTCTCCG AATTGTACAC ATATCACGCG TTAAGTCgac aatcaacctc tggattacaa 2761 aatttgtgaa agatt