Construct: ORF TRCN0000475150
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000135.1_s317c1
- Derived from:
- ccsbBroadEn_00360
- DNA Barcode:
- GGTGTGTATCCTCGACAATTCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CREB1 (1385)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475150
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1385 | CREB1 | cAMP responsive element bin... | NM_001371426.1 | 100% | 100% | |
| 2 | human | 1385 | CREB1 | cAMP responsive element bin... | NM_134442.5 | 100% | 100% | |
| 3 | human | 1385 | CREB1 | cAMP responsive element bin... | NM_001371427.1 | 95.8% | 95.8% | 258_259ins42 |
| 4 | human | 1385 | CREB1 | cAMP responsive element bin... | NM_004379.5 | 95.8% | 95.8% | 258_259ins42 |
| 5 | human | 1385 | CREB1 | cAMP responsive element bin... | XM_011510648.3 | 89.2% | 86.2% | (many diffs) |
| 6 | human | 1385 | CREB1 | cAMP responsive element bin... | XM_011510650.3 | 88.2% | 88.2% | 0_1ins120 |
| 7 | human | 1385 | CREB1 | cAMP responsive element bin... | XM_017003399.2 | 85.1% | 82.1% | (many diffs) |
| 8 | human | 1385 | CREB1 | cAMP responsive element bin... | NM_001371428.1 | 84.1% | 84.1% | 0_1ins120;138_139ins42 |
| 9 | human | 1385 | CREB1 | cAMP responsive element bin... | XM_011510646.3 | 83.1% | 87.2% | 880_965del;1008_1009ins101 |
| 10 | human | 1385 | CREB1 | cAMP responsive element bin... | NM_001320793.2 | 68% | 67.4% | (many diffs) |
| 11 | human | 1385 | CREB1 | cAMP responsive element bin... | XM_017003401.2 | 29.8% | 29% | (many diffs) |
| 12 | human | 1385 | CREB1 | cAMP responsive element bin... | XR_241292.2 | 13.3% | 1_200del;930_992del;1287_7662del | |
| 13 | human | 1385 | CREB1 | cAMP responsive element bin... | XR_241290.2 | 13.1% | 1_200del;928_1105del;1402_7777del | |
| 14 | human | 1385 | CREB1 | cAMP responsive element bin... | XR_001738636.2 | 12.4% | 1_200del;928_1521del;1818_8193del | |
| 15 | human | 1385 | CREB1 | cAMP responsive element bin... | XR_001738637.2 | 12% | (many diffs) | |
| 16 | human | 1385 | CREB1 | cAMP responsive element bin... | NR_135473.2 | 11.9% | 1_181del;1205_8541del | |
| 17 | human | 1385 | CREB1 | cAMP responsive element bin... | NR_163947.1 | 11.6% | 1_65del;323_324ins42;1047_8383del | |
| 18 | human | 1385 | CREB1 | cAMP responsive element bin... | NR_163946.1 | 11.4% | 1_181del;439_440ins42;1163_8499del | |
| 19 | mouse | 12912 | Creb1 | cAMP responsive element bin... | NM_009952.2 | 95.3% | 99.7% | (many diffs) |
| 20 | mouse | 12912 | Creb1 | cAMP responsive element bin... | XM_006495650.3 | 95.3% | 99.7% | (many diffs) |
| 21 | mouse | 12912 | Creb1 | cAMP responsive element bin... | XM_017314080.1 | 95.3% | 99.7% | (many diffs) |
| 22 | mouse | 12912 | Creb1 | cAMP responsive element bin... | NM_133828.2 | 91.3% | 95.6% | (many diffs) |
| 23 | mouse | 12912 | Creb1 | cAMP responsive element bin... | XM_006495651.3 | 91.3% | 95.6% | (many diffs) |
| 24 | mouse | 12912 | Creb1 | cAMP responsive element bin... | XM_017314099.1 | 91.3% | 95.6% | (many diffs) |
| 25 | mouse | 12912 | Creb1 | cAMP responsive element bin... | XM_006495652.3 | 84% | 88.2% | (many diffs) |
| 26 | mouse | 12912 | Creb1 | cAMP responsive element bin... | NM_001037726.1 | 80% | 84.1% | (many diffs) |
| 27 | mouse | 12912 | Creb1 | cAMP responsive element bin... | XM_006495653.3 | 77.9% | 81.5% | (many diffs) |
| 28 | mouse | 12912 | Creb1 | cAMP responsive element bin... | XM_006495654.3 | 73.9% | 77.4% | (many diffs) |
| 29 | mouse | 12912 | Creb1 | cAMP responsive element bin... | XM_011238424.2 | 37.2% | 39% | (many diffs) |
| 30 | mouse | 12912 | Creb1 | cAMP responsive element bin... | XM_011238425.2 | 37.2% | 39% | (many diffs) |
| 31 | mouse | 12912 | Creb1 | cAMP responsive element bin... | XM_006495655.3 | 28.5% | 29% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1089
- ORF length:
- 1023
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac catggaatct ggagccgaga accagcagag tggagatgca gctgtaacag 121 aagctgaaaa ccaacaaatg acagttcaag cccagccaca gattgccaca ttagcccagg 181 tatctatgcc agcagctcat gcaacatcat ctgctcccac cgtaactcta gtacagctgc 241 ccaatgggca gacagttcaa gtccatggag tcattcaggc ggcccagcca tcagttattc 301 agtctccaca agtccaaaca gttcagtctt cctgtaagga cttaaaaaga cttttctccg 361 gaacacagat ttcaactatt gcagaaagtg aagattcaca ggagtcagtg gatagtgtaa 421 ctgattccca aaagcgaagg gaaattcttt caaggaggcc ttcctacagg aaaattttga 481 atgacttatc ttcTGATGCA CCAGGAGTGC CAAGGATTGA AGAAGAGAAG TCTGAAGAGG 541 AGACTTCAGC ACCTGCCATC ACCACTGTAA CGGTGCCAAC TCCAATTTAC CAAACTAGCA 601 GTGGACAGTA TATTGCCATT ACCCAGGGAG GAGCAATACA GCTGGCTAAC AATGGTACCG 661 ATGGGGTACA GGGCCTGCAA ACATTAACCA TGACCAATGC AGCAGCCACT CAGCCGGGTA 721 CTACCATTCT ACAGTATGCA CAGACCACTG ATGGACAGCA GATCTTAGTG CCCAGCAACC 781 AAGTTGTTGT TCAAGCTGCC TCTGGAGACG TACAAACATA CCAGATTCGC ACAGCACCCA 841 CTAGCACTAT TGCCCCTGGA GTTGTTATGG CATCCTCCCC AGCACTTCCT ACACAGCCTG 901 CTGAAGAAGC AGCACGAAAG AGAGAGGTCC GTCTAATGAA GAACAGGGAA GCAGCTCGAG 961 AGTGTCGTAG AAAGAAGAAA GAATATGTGA AATGTTTAGA AAACAGAGTG GCAGTGCTTG 1021 AAAATCAAAA CAAGACATTG ATTGAGGAGC TAAAAGCACT TAAGGACCTT TACTGCCACA 1081 AATCAGATTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1141 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1201 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGGTGTG TATCCTCGAC AATTCGGGAC 1261 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt