Construct: ORF TRCN0000476385
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003785.1_s317c1
- Derived from:
- ccsbBroadEn_14299
- DNA Barcode:
- CTGCAAATATAGATCAGTGTTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNX25 (83891)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476385
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83891 | SNX25 | sorting nexin 25 | XM_024454240.1 | 89.1% | 89% | (many diffs) |
| 2 | human | 83891 | SNX25 | sorting nexin 25 | XM_017008685.2 | 83% | 80.8% | (many diffs) |
| 3 | human | 83891 | SNX25 | sorting nexin 25 | XM_017008684.2 | 79.5% | 79.5% | (many diffs) |
| 4 | human | 83891 | SNX25 | sorting nexin 25 | XM_017008683.2 | 75.9% | 75.9% | (many diffs) |
| 5 | human | 83891 | SNX25 | sorting nexin 25 | NM_001317781.1 | 66.1% | 66% | (many diffs) |
| 6 | human | 83891 | SNX25 | sorting nexin 25 | XM_011532308.3 | 56.5% | 56.5% | 1_1278del;1746T>C;2082G>A |
| 7 | human | 83891 | SNX25 | sorting nexin 25 | XM_005263267.5 | 55.3% | 55.2% | (many diffs) |
| 8 | human | 83891 | SNX25 | sorting nexin 25 | XM_011532305.3 | 54.6% | 54.6% | (many diffs) |
| 9 | human | 83891 | SNX25 | sorting nexin 25 | XM_011532304.3 | 53.5% | 53.5% | (many diffs) |
| 10 | human | 83891 | SNX25 | sorting nexin 25 | XM_011532303.3 | 52.9% | 52.9% | (many diffs) |
| 11 | human | 83891 | SNX25 | sorting nexin 25 | XM_011532302.3 | 52.9% | 52.9% | (many diffs) |
| 12 | human | 83891 | SNX25 | sorting nexin 25 | XM_017008680.1 | 49.4% | 48.5% | (many diffs) |
| 13 | human | 83891 | SNX25 | sorting nexin 25 | XM_011532306.2 | 48.8% | 47.9% | (many diffs) |
| 14 | human | 83891 | SNX25 | sorting nexin 25 | XR_938776.2 | 37.1% | (many diffs) | |
| 15 | mouse | 102141 | Snx25 | sorting nexin 25 | XM_017312502.1 | 68.8% | 69.5% | (many diffs) |
| 16 | mouse | 102141 | Snx25 | sorting nexin 25 | NM_207213.2 | 56.2% | 56.8% | (many diffs) |
| 17 | mouse | 102141 | Snx25 | sorting nexin 25 | XM_006509243.3 | 56.2% | 56.8% | (many diffs) |
| 18 | mouse | 102141 | Snx25 | sorting nexin 25 | XM_006509242.3 | 47.9% | 48.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1737
- ORF length:
- 1668
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaagctgat ctcctgaggg ccaggaacat gaagaggtac atcaaccaac 121 tgactgtggc aaagaagcag tgtgagaaga gaatccgaat cctgggaggc cctgcctatg 181 accagcaaga ggatggggcc ctggatgagg gggaagggcc tcaaagccag aagattcttc 241 agtttgaaga tatcttggcc aatacgttct accgagagca ctttggaatg tacatggaaa 301 ggatggacaa aagagctctg attagttttt gggaatctgt ggaacattta aagaatgcta 361 acaagaatga aattccacaa ttagttggtg aaatttatca gaatttcttt gtggagagca 421 aagaaatatc tgtggaaaaa tcactttaca aagaaattca gcagtgtctt gtaggaaata 481 aaggtattga agtattctac aaaatccagg aagatgttta tgagacccta aaggacaggt 541 attacccttc atttattgtc agtgacctgt atgagaaatt gttgataaaa gaggaagaaa 601 aacatgcctc acagatgatt tccaacaagg atgagatggg cccaagagat gaggctggtg 661 aggaagccgt ggatgatggt accaatcaga tcaatgaaca agccagtttt gctgtaaaca 721 aactgcgaga actaaatgag aaacttgaat ataaaaggca agctctaaat tctattcaaa 781 atgcaccaaa acctgacaag aagattgttt ccaagttgaa ggatgaaata atcctaatag 841 agaaagaacg cacagacctt cagctgcaca tagcaagaac ggattggtgg tgtgaaaacc 901 ttggcatgtg gaaagcctcc atcaccagtg gagaggttac agaagagaat ggtgagcaat 961 tgccatgtta ctttgtcatg gtaagcctac aagaagttgg aggagttgaa actaagaact 1021 ggacggtccc cagaaggctc agcgagtttc agaatttaca ccggaaactc agtgagtgcg 1081 tcccttcttt aaaaaaagtc cagttgcctt ctcttagcaa gctgcctttc aaatctatag 1141 atcaaaagtt tatggaaaag tcgaagaatc aattaaataa gtttttacag gaggagacag 1201 aggaggacag tgacctgtca gattatggtg atgatgtgga tgggaggaaa gacgccttgg 1261 ctgaaccatg tttcatgttg attggggaga tttttgaact tcgaggaatg tttaaatggg 1321 tgagaagaac attaattgcc ctcgttcagg tcacttttgg aagaaccatc aacaaacaaa 1381 TCCGGGACAC AGTCAGCTGG ATTTTCAGTG AGCAAATGTT GGTTTACTAC ATCAATATTT 1441 TCCGGGATGC TTTTTGGCCA AATGGGAAGT TGGCACCACC GACCACAATC AGAAGCAAAG 1501 AGCAAAGTCA GGAAACAAAA CAGAGAGCAC AGCAAAAGCT GCTTGAAAAC ATTCCAGATA 1561 TGCTTCAGAG CCTTGTTGGA CAGCAAAATG CCCGCCACGG TATAATAAAA ATATTCAATG 1621 CACTGCAAGA AACAAGAGCC AACAAGCATC TGTTATATGC GCTGATGGAA CTGCTGCTAA 1681 TTGAACTGTG TCCTGAGCTG AGAGTTCATT TAGATCAACT TAAAGCTGGC CAAGTTTTGC 1741 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1801 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1861 ATATATCTTG TGGAAAGGAC GACTGCAAAT ATAGATCAGT GTTTCAACGC GTTAAGTCga 1921 caatcaacct ctggattaca aaatttgtga aagatt