Construct: ORF TRCN0000476385

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003785.1_s317c1
Derived from:
ccsbBroadEn_14299
DNA Barcode:
CTGCAAATATAGATCAGTGTTTCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SNX25 (83891)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476385
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 83891 SNX25 sorting nexin 25 XM_024454240.1 89.1% 89% (many diffs)
2 human 83891 SNX25 sorting nexin 25 XM_017008685.2 83% 80.8% (many diffs)
3 human 83891 SNX25 sorting nexin 25 XM_017008684.2 79.5% 79.5% (many diffs)
4 human 83891 SNX25 sorting nexin 25 XM_017008683.2 75.9% 75.9% (many diffs)
5 human 83891 SNX25 sorting nexin 25 NM_001317781.1 66.1% 66% (many diffs)
6 human 83891 SNX25 sorting nexin 25 XM_011532308.3 56.5% 56.5% 1_1278del;1746T>C;2082G>A
7 human 83891 SNX25 sorting nexin 25 XM_005263267.5 55.3% 55.2% (many diffs)
8 human 83891 SNX25 sorting nexin 25 XM_011532305.3 54.6% 54.6% (many diffs)
9 human 83891 SNX25 sorting nexin 25 XM_011532304.3 53.5% 53.5% (many diffs)
10 human 83891 SNX25 sorting nexin 25 XM_011532303.3 52.9% 52.9% (many diffs)
11 human 83891 SNX25 sorting nexin 25 XM_011532302.3 52.9% 52.9% (many diffs)
12 human 83891 SNX25 sorting nexin 25 XM_017008680.1 49.4% 48.5% (many diffs)
13 human 83891 SNX25 sorting nexin 25 XM_011532306.2 48.8% 47.9% (many diffs)
14 human 83891 SNX25 sorting nexin 25 XR_938776.2 37.1% (many diffs)
15 mouse 102141 Snx25 sorting nexin 25 XM_017312502.1 68.8% 69.5% (many diffs)
16 mouse 102141 Snx25 sorting nexin 25 NM_207213.2 56.2% 56.8% (many diffs)
17 mouse 102141 Snx25 sorting nexin 25 XM_006509243.3 56.2% 56.8% (many diffs)
18 mouse 102141 Snx25 sorting nexin 25 XM_006509242.3 47.9% 48.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1737
ORF length:
1668
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaaagctgat ctcctgaggg ccaggaacat gaagaggtac atcaaccaac
121tgactgtggc aaagaagcag tgtgagaaga gaatccgaat cctgggaggc cctgcctatg
181accagcaaga ggatggggcc ctggatgagg gggaagggcc tcaaagccag aagattcttc
241agtttgaaga tatcttggcc aatacgttct accgagagca ctttggaatg tacatggaaa
301ggatggacaa aagagctctg attagttttt gggaatctgt ggaacattta aagaatgcta
361acaagaatga aattccacaa ttagttggtg aaatttatca gaatttcttt gtggagagca
421aagaaatatc tgtggaaaaa tcactttaca aagaaattca gcagtgtctt gtaggaaata
481aaggtattga agtattctac aaaatccagg aagatgttta tgagacccta aaggacaggt
541attacccttc atttattgtc agtgacctgt atgagaaatt gttgataaaa gaggaagaaa
601aacatgcctc acagatgatt tccaacaagg atgagatggg cccaagagat gaggctggtg
661aggaagccgt ggatgatggt accaatcaga tcaatgaaca agccagtttt gctgtaaaca
721aactgcgaga actaaatgag aaacttgaat ataaaaggca agctctaaat tctattcaaa
781atgcaccaaa acctgacaag aagattgttt ccaagttgaa ggatgaaata atcctaatag
841agaaagaacg cacagacctt cagctgcaca tagcaagaac ggattggtgg tgtgaaaacc
901ttggcatgtg gaaagcctcc atcaccagtg gagaggttac agaagagaat ggtgagcaat
961tgccatgtta ctttgtcatg gtaagcctac aagaagttgg aggagttgaa actaagaact
1021ggacggtccc cagaaggctc agcgagtttc agaatttaca ccggaaactc agtgagtgcg
1081tcccttcttt aaaaaaagtc cagttgcctt ctcttagcaa gctgcctttc aaatctatag
1141atcaaaagtt tatggaaaag tcgaagaatc aattaaataa gtttttacag gaggagacag
1201aggaggacag tgacctgtca gattatggtg atgatgtgga tgggaggaaa gacgccttgg
1261ctgaaccatg tttcatgttg attggggaga tttttgaact tcgaggaatg tttaaatggg
1321tgagaagaac attaattgcc ctcgttcagg tcacttttgg aagaaccatc aacaaacaaa
1381TCCGGGACAC AGTCAGCTGG ATTTTCAGTG AGCAAATGTT GGTTTACTAC ATCAATATTT
1441TCCGGGATGC TTTTTGGCCA AATGGGAAGT TGGCACCACC GACCACAATC AGAAGCAAAG
1501AGCAAAGTCA GGAAACAAAA CAGAGAGCAC AGCAAAAGCT GCTTGAAAAC ATTCCAGATA
1561TGCTTCAGAG CCTTGTTGGA CAGCAAAATG CCCGCCACGG TATAATAAAA ATATTCAATG
1621CACTGCAAGA AACAAGAGCC AACAAGCATC TGTTATATGC GCTGATGGAA CTGCTGCTAA
1681TTGAACTGTG TCCTGAGCTG AGAGTTCATT TAGATCAACT TAAAGCTGGC CAAGTTTTGC
1741CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1801TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1861ATATATCTTG TGGAAAGGAC GACTGCAAAT ATAGATCAGT GTTTCAACGC GTTAAGTCga
1921caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)