Construct: ORF TRCN0000476622
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006799.1_s317c1
- Derived from:
- ccsbBroadEn_09966
- DNA Barcode:
- TTTTACCGTGCTGGCCAAGGACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NBPF15 (284565)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476622
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284565 | NBPF15 | NBPF member 15 | NM_001170755.2 | 99.9% | 99.8% | 1335G>A;1430A>C |
| 2 | human | 284565 | NBPF15 | NBPF member 15 | NM_173638.4 | 99.9% | 99.8% | 1335G>A;1430A>C |
| 3 | human | 84224 | NBPF3 | NBPF member 3 | NM_001330381.2 | 77.9% | 69.1% | (many diffs) |
| 4 | human | 84224 | NBPF3 | NBPF member 3 | XM_006710958.1 | 77.9% | 69.1% | (many diffs) |
| 5 | human | 84224 | NBPF3 | NBPF member 3 | XM_006710959.2 | 77.9% | 69.1% | (many diffs) |
| 6 | human | 84224 | NBPF3 | NBPF member 3 | XM_011542276.1 | 77.9% | 69.1% | (many diffs) |
| 7 | human | 84224 | NBPF3 | NBPF member 3 | XM_011542277.1 | 77.9% | 69.1% | (many diffs) |
| 8 | human | 84224 | NBPF3 | NBPF member 3 | XM_011542278.1 | 77.9% | 69.1% | (many diffs) |
| 9 | human | 84224 | NBPF3 | NBPF member 3 | XM_011542281.1 | 77.9% | 69.1% | (many diffs) |
| 10 | human | 84224 | NBPF3 | NBPF member 3 | XM_017002499.2 | 77.9% | 69.1% | (many diffs) |
| 11 | human | 84224 | NBPF3 | NBPF member 3 | XM_024450188.1 | 77.9% | 69.1% | (many diffs) |
| 12 | human | 84224 | NBPF3 | NBPF member 3 | XM_024450190.1 | 77.9% | 69.1% | (many diffs) |
| 13 | human | 84224 | NBPF3 | NBPF member 3 | XM_017002500.1 | 73% | 65% | (many diffs) |
| 14 | human | 84224 | NBPF3 | NBPF member 3 | XM_017002501.1 | 73% | 65% | (many diffs) |
| 15 | human | 84224 | NBPF3 | NBPF member 3 | XM_017002502.1 | 73% | 65% | (many diffs) |
| 16 | human | 84224 | NBPF3 | NBPF member 3 | NM_001256417.3 | 73% | 61.5% | (many diffs) |
| 17 | human | 84224 | NBPF3 | NBPF member 3 | NM_032264.5 | 71.9% | 63.7% | (many diffs) |
| 18 | human | 400818 | NBPF9 | NBPF member 9 | NM_001037675.3 | 69.8% | 68.6% | (many diffs) |
| 19 | human | 728841 | NBPF8 | NBPF member 8 | NM_001037501.3 | 69.7% | 68.3% | (many diffs) |
| 20 | human | 102724250 | LOC102724250 | neuroblastoma breakpoint fa... | XM_024452498.1 | 69.1% | 67.2% | (many diffs) |
| 21 | human | 84224 | NBPF3 | NBPF member 3 | XM_006710957.1 | 67.4% | 60% | (many diffs) |
| 22 | human | 84224 | NBPF3 | NBPF member 3 | NM_001256416.3 | 65.1% | 58% | (many diffs) |
| 23 | human | 200030 | NBPF11 | NBPF member 11 | NM_001101663.4 | 61.5% | 53% | (many diffs) |
| 24 | human | 400818 | NBPF9 | NBPF member 9 | NM_001277444.1 | 59.3% | 58.5% | (many diffs) |
| 25 | human | 200030 | NBPF11 | NBPF member 11 | NM_183372.6 | 58.2% | 50.2% | (many diffs) |
| 26 | human | 102724250 | LOC102724250 | neuroblastoma breakpoint fa... | XM_017030107.2 | 56.3% | 27% | (many diffs) |
| 27 | human | 149013 | NBPF12 | NBPF member 12 | XM_024453443.1 | 55% | 53.6% | (many diffs) |
| 28 | human | 55672 | NBPF1 | NBPF member 1 | NM_017940.6 | 53.7% | 52.2% | (many diffs) |
| 29 | human | 101929780 | NBPF25P | NBPF member 25, pseudogene | NR_104217.1 | 51.5% | (many diffs) | |
| 30 | human | 200030 | NBPF11 | NBPF member 11 | XM_024453858.1 | 49.7% | 45.8% | (many diffs) |
| 31 | human | 101060684 | NBPF26 | NBPF member 26 | NM_001351372.1 | 49.4% | 48.3% | (many diffs) |
| 32 | human | 149013 | NBPF12 | NBPF member 12 | NM_001278141.1 | 44.8% | 43.6% | (many diffs) |
| 33 | human | 200030 | NBPF11 | NBPF member 11 | XR_002959662.1 | 38.2% | (many diffs) | |
| 34 | human | 200030 | NBPF11 | NBPF member 11 | XR_002959669.1 | 38% | (many diffs) | |
| 35 | human | 343505 | NBPF7 | NBPF member 7 | XM_017001186.1 | 37% | 28.9% | (many diffs) |
| 36 | human | 200030 | NBPF11 | NBPF member 11 | XR_002959670.1 | 36.3% | (many diffs) | |
| 37 | human | 200030 | NBPF11 | NBPF member 11 | NR_110864.2 | 35.2% | (many diffs) | |
| 38 | human | 84224 | NBPF3 | NBPF member 3 | NR_046176.3 | 33.4% | (many diffs) | |
| 39 | human | 200030 | NBPF11 | NBPF member 11 | NR_046188.4 | 32.8% | (many diffs) | |
| 40 | human | 200030 | NBPF11 | NBPF member 11 | XM_024453859.1 | 32% | 29.2% | (many diffs) |
| 41 | human | 728841 | NBPF8 | NBPF member 8 | NR_102405.2 | 24.8% | (many diffs) | |
| 42 | human | 728841 | NBPF8 | NBPF member 8 | NR_102404.2 | 24% | (many diffs) | |
| 43 | human | 25832 | NBPF14 | NBPF member 14 | NM_015383.2 | 22.7% | 21.3% | (many diffs) |
| 44 | human | 100132406 | NBPF10 | NBPF member 10 | NM_001039703.6 | 17.6% | 16.6% | (many diffs) |
| 45 | human | 100132406 | NBPF10 | NBPF member 10 | NM_001302371.3 | 16.8% | 15.9% | (many diffs) |
| 46 | human | 101060226 | NBPF19 | NBPF member 19 | NM_001351365.1 | 16.6% | 15.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2076
- ORF length:
- 2010
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggtatcagcc ggccctttgt ccagcgagaa ggcagagatg aacattctag 121 aaatcaatga gaaattgcgc ccccagttgg cagagaagaa acagcagttc agaaacctca 181 aagagaaatg ttttctaact caactggccg gcttcctggc caaccgacag aagaaataca 241 aatatgaaga gtgcaaagat ctcataaaat ttatgctgag gaatgagcga cagttcaagg 301 aggagaagct tgcagagcag ctcaagcaag ctgaggagct caggcaatat aaagtcctgg 361 ttcacgctca ggaacgagag ctgacccagt taagggagaa gttacgggaa gggagagatg 421 cctcccgctc attgaatgag catctccagg ccctcctcac tccggatgag ccggacaagt 481 cccaggggca ggacctccaa gaacagctgg ctgaggggtg tagactgaca cagcaccttg 541 tccaaaagct cagcccagaa aatgacaacg atgacgatga agatgttcaa gttgaggtgg 601 ctgagaaagt gcagaaatcg tctgccccca gggagatgca gaaggctgaa gaaaaggaag 661 tccctgagga ctcactggag gaatgtgcca tcacttgttc aaatagccat ggcccttatg 721 actccaacca gccacataag aaaaccaaaa tcacatttga ggaagacaaa gtcgactcaa 781 ctctcattgg ctcatcctct catgttgaat gggaggatgc tgtacacatt attccagaaa 841 atgaaagtga tgatgaggaa gaggaagaaa aagggccagt gtctcccagg aatctgcagg 901 agtctgaaga ggaggaagtc ccccaggagt cctgggatga aggttattcg actctctcaa 961 ttcctcctga aatgttggcc tcgtaccagt cttacagcag cacatttcac tcattagagg 1021 aacagcaagt ctgcatggct gttgacatag gcagacatcg gtgggatcaa gtgaaaaagg 1081 aggaccaaga ggcaacaggt cccaggctca gcagggagct gctggatgag aaagagcctg 1141 aagtcttgca ggactcactg gatagatgtt attcaactcc ttcaggttgt cttgaactga 1201 ctgactcatg ccagccctac agaagtgcct tttacgtatt ggagcaacag cgtgttggct 1261 tggctattga catggatgaa attgaaaagt accaagaagt ggaagaagac caagacccat 1321 catgccccag gctcagcagg gagctgctgg atgagaaaga gcctgaagtc ttgcaggact 1381 cactggatag atgttattca actccttcag attatcttga actgcctgac ttaggccagc 1441 cctacagcag tgctgtttac tcattggagg aacagtacct tggcttggct cttgccgtgg 1501 acagaattaa aaaggaccaa gaagaggaag aagaccaagg cccaccatgc cccaggctca 1561 gcagggagct gctggaggta gtagagcctg aagtcttgca ggactcactg gatagatgtt 1621 attcaactcc ttccagttgt cttgaacagc ctgactcctg ccagccctat ggaagttcct 1681 tttatgcatt GGAGGAAAAA CATGTTGGCT TTTCTCTTGA CGTGGGAGAA ATTGAAAAGA 1741 AGGGGAAGGG GAAGAAAAGA AGGGGAAGAA GATCAAAGAA GAAAAGAAGA AGGGGAAGAA 1801 AAGAAGGGGA AGATGACAAC CCACCATGCC CCAGGCTCTA CGGCGTGCTG ATGGAAGTGG 1861 AAGAGCCTGA AGTCTTACAG GACTCACTGG ATAGATGTTA TTCGACTCCG TCAATGTACT 1921 TTGAACAACC TGACTCATTC CAGCACTACA GAAGTGTGTT TTACTCATTT GAGGAAGAGC 1981 ATATCAGCTT CGCCCTTTAC GTGGACAATA GGTTTTTTAC TTTGACGGTG ACAAGTCTCC 2041 ACCTGGTGTT CCAGATGGGA GTCATATTCC CACAATACCC AACTTTCTTG TACAAAGTGG 2101 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2161 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2221 ATTTTACCGT GCTGGCCAAG GACTTACGCG TTAAGTCgac aatcaacctc tggattacaa 2281 aatttgtgaa agatt