Construct: ORF TRCN0000477107
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012377.1_s317c1
- Derived from:
- ccsbBroadEn_13623
- DNA Barcode:
- CCCCAGACCGATGGGTCGTTCCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CASTOR3 (352954)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477107
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 352954 | CASTOR3 | CASTOR family member 3 | NM_178831.6 | 99.5% | 99.3% | 231G>A;233A>G |
| 2 | human | 352954 | CASTOR3 | CASTOR family member 3 | XM_017012158.2 | 80.4% | 75.5% | (many diffs) |
| 3 | human | 352954 | CASTOR3 | CASTOR family member 3 | XM_017012157.1 | 80% | 75.1% | (many diffs) |
| 4 | human | 352954 | CASTOR3 | CASTOR family member 3 | XM_017012156.1 | 79.8% | 72.3% | (many diffs) |
| 5 | human | 352954 | CASTOR3 | CASTOR family member 3 | XM_017012155.1 | 69.4% | 64.9% | (many diffs) |
| 6 | human | 352954 | CASTOR3 | CASTOR family member 3 | XM_017012154.1 | 67% | 62.6% | (many diffs) |
| 7 | human | 352954 | CASTOR3 | CASTOR family member 3 | XM_017012153.1 | 65.8% | 61.5% | (many diffs) |
| 8 | human | 352954 | CASTOR3 | CASTOR family member 3 | XM_017012159.1 | 63.6% | 63.5% | 1_276del;507G>A;509A>G |
| 9 | human | 352954 | CASTOR3 | CASTOR family member 3 | XM_024446753.1 | 63.6% | 63.5% | 1_276del;507G>A;509A>G |
| 10 | human | 729438 | CASTOR2 | cytosolic arginine sensor f... | XM_017012574.1 | 48.8% | 38.2% | (many diffs) |
| 11 | human | 729438 | CASTOR2 | cytosolic arginine sensor f... | NM_001145064.3 | 43.2% | 33.9% | (many diffs) |
| 12 | human | 352954 | CASTOR3 | CASTOR family member 3 | NR_028040.1 | 16.9% | (many diffs) | |
| 13 | human | 352954 | CASTOR3 | CASTOR family member 3 | XR_001744712.1 | 16.2% | (many diffs) | |
| 14 | human | 352954 | CASTOR3 | CASTOR family member 3 | XR_001744713.1 | 15.1% | (many diffs) | |
| 15 | human | 352954 | CASTOR3 | CASTOR family member 3 | NR_028039.1 | 13.6% | (many diffs) | |
| 16 | human | 352954 | CASTOR3 | CASTOR family member 3 | XR_001744710.1 | 12.7% | (many diffs) | |
| 17 | human | 352954 | CASTOR3 | CASTOR family member 3 | NR_028038.2 | 12% | (many diffs) | |
| 18 | human | 352954 | CASTOR3 | CASTOR family member 3 | XR_001744711.1 | 11.7% | (many diffs) | |
| 19 | human | 352954 | CASTOR3 | CASTOR family member 3 | XR_002956435.1 | 10.4% | (many diffs) | |
| 20 | human | 352954 | CASTOR3 | CASTOR family member 3 | XR_002956433.1 | 9.4% | (many diffs) | |
| 21 | human | 352954 | CASTOR3 | CASTOR family member 3 | XR_002956434.1 | 9% | (many diffs) | |
| 22 | mouse | 80909 | Gatsl2 | GATS protein-like 2 | NM_030719.3 | 39.7% | 33.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 558
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagctgccgc ggacggggcg cgggcggacg atggaactcc acatcctgga 121 gcaccggctg caagttgcca gcgtcgccaa ggagagtatc ccgctgttca cctacgggcc 181 tgatcaaact tgccttcctg ttctccaaga ccaggtgcaa gttcTTCAGT CTGACTGAGA 241 CACCAGAGGA TTACACTATC ATTGTCGATG AGGAAGGATT CCTAGAGCTG CCCTCCTCAG 301 GGCACCTGAG TGTGGCGGAT GCCACCTGGC TGGCCCTGAA CGTGGTGTCC GGCGGTGGCA 361 GCTTCTCCAG CTCCCAGCCT ATTGGCATGA CCAAGATCGC CAAGTCAGTC ATCGCCCCAC 421 TGGCTGACCA GAACATATCC GTGTTCATGC TGTCCACGTA TCAGACAGAC TTCATCCTGG 481 TGCTCAAGCG GGACCTGCCC TTTGTCACCC ACACGTTGTC ATCAGAGTTC ACCATCCTGT 541 GGTCAGTGGC TAGACTGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCCCAGA CCGATGGGTC 721 GTTCCATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt