Construct: ORF TRCN0000477515
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018549.1_s317c1
- Derived from:
- ccsbBroadEn_13920
- DNA Barcode:
- CGGGTATATCCCGATTCTAAAAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PLCL1 (5334)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477515
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5334 | PLCL1 | phospholipase C like 1 (ina... | XM_017004340.2 | 99.8% | .8% | 11delA;1705G>A;1745T>A |
| 2 | human | 5334 | PLCL1 | phospholipase C like 1 (ina... | XM_005246644.4 | 98% | .7% | (many diffs) |
| 3 | human | 5334 | PLCL1 | phospholipase C like 1 (ina... | XM_011511351.2 | 98% | .7% | (many diffs) |
| 4 | human | 5334 | PLCL1 | phospholipase C like 1 (ina... | XM_017004339.2 | 98% | .7% | (many diffs) |
| 5 | human | 5334 | PLCL1 | phospholipase C like 1 (ina... | XM_005246643.4 | 97.5% | .7% | (many diffs) |
| 6 | human | 5334 | PLCL1 | phospholipase C like 1 (ina... | NM_006226.4 | 90.9% | .7% | (many diffs) |
| 7 | mouse | 227120 | Plcl1 | phospholipase C-like 1 | NM_001114663.1 | 80.1% | .7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 135
- ORF length:
- 66
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccatcggaa agaaaattag cagtgcaaat gactgcatca gcttcatgca 121 agctggctgt gagttgaaga aagtccggcc aaattctcgc atttacaacc gttttttcac 181 tctggacaca gaccttcaag ctcttcgctg ggaaccttca aagaaagacc tcgagaaagc 241 caagcttgat atttctgcca taaaagagat cagactgggg aaaaacacgg aaacatttag 301 aaacaatggc cttgctgacc agatctgtga ggactgtgcc ttttccatac tccacgggga 361 aaactatgag tctctggacc tagttgccaa ttcagcagat gtggcaaaca tctgggtgtc 421 tgggttacgg tacctggttt ctcgaagtaa gcagcctctt gattttatgg agggcaacca 481 gaacacacca cggttcatgt ggttgaaaac agtgtttgaa gcagcagatg ttgatgggaa 541 tgggattatg ttggaagaca cctctgtaga gttaataaaa caactcaacc ctactctgaa 601 ggaagccaag atcaggttaa agtttaaaga aatccagaag agcaaggaaa aactaaccac 661 ccgcgtgacc gaagaggaat tttgtgaagc tttttgtgaa ctttgcacca ggccagaagt 721 gtatttctta cttgtacaga tatctaaaaa caaagaatat ttggatgcca atgatctcat 781 gctcttttta gaagctgagc aaggagtcac ccatatcacc gaggatatat gcttagacat 841 cataaggaga tacgaacttt ctgaagaggg acgtcaaaaa gggtttcttg caattgatgg 901 ctttacccag tatttattgt catcagaatg tgacattttt gatcctgagc aaaagaaggt 961 tgcccaagat atgacccagc cattatctca ctactatatc aatgcctctc ataacaccta 1021 tctaatagaa gaccagttca gggggccagc tgacatcaat gggtacatta gagctttgaa 1081 aatgggctgt cgaagcgttg aactcgatgt aagtgatggt tcagataatg aaccaatcct 1141 ttgtaatcga aataacatga caacccatgt ttcctttcga agtgtcatag aggtaataaa 1201 taaatttgcc tttgttgctt ctgaataccc actcattctt tgcttgggaa atcactgctc 1261 cttgccgcag cagaaggtaa tggctcaaca gatgaaaaag gtctttggca ataaactcta 1321 tactgaagca cctttgccct cagaatccta cctcccatca ccagaaaaat taaaaagaat 1381 gatcattgtg aaaggaaaga agttgccttc tgatccagat gtgttagaag gagaagtaac 1441 agatgaagat gaagaagctg aaatgtctcg aaggatgtcg gtagattaca atggtgagca 1501 gaagcaaatc cgactctgta gggagctctc tgatttggtg tctatttgta aatctgttca 1561 atacagggat tttgaactat ctatgaaaag ccaaaactat tgggaaatgt gttcatttag 1621 tgaaacagag gccagccgca ttgcaaatga gtacccagag gattttgtta attataataa 1681 gaagttctta tcaagaatct atccaagtgc catgaggatc gattccagta acttgaatcc 1741 acaggacttt tggaattgtg gctgtcagat tatagcaatg aattttcaga ctccgggtcc 1801 aatgatggac catcacacgg gctggtttct tcaaaacggg ggatgtggtt atgttctaag 1861 gccgtctata atgcgagatg aagtttctta cttcagcgca aatacaaagg gcattctacc 1921 tggggtgtct cctctagctc ttcatatcaa gatcatcagt ggtcagaatt tcccaaagcc 1981 caagggagct tgtgccaaag gggatgtcat agatccctat gtttgtatag agatacacgg 2041 aattccagcg gattgttcgg aacaaagaac taaaactgta cagcaaaaca gtgataatcc 2101 tatttttgat gaaacttttg agttccaagt aaacctacct gagctggcca tgatccgttt 2161 tgttgttctg gatgatgact acattgggga tgagtttata gggcaatata cgataccatt 2221 tgaatgtttg cagcctggat atcggcatgt tcccctgcgt tcttttgtgg gtgacatcat 2281 ggagcacgta accctttttg tccacatagc aataactaat cgaagtggag gaggaaaggc 2341 acagaagcgc agtctttcag tgagaatggg gaagaaagtt cgggaatata ccatgctcag 2401 gaatatcggt cttaaaacca ttgatgacat ctttaaaata gcggttcatc cattacgaga 2461 agccatagat atgagagaaa atatgcagaa tgcaatcgtg tctattaagg aactatgtgg 2521 actccctcca attgccagtc tgaagcagtg cctgttaact ctgtcatctc ggctcatcac 2581 cagtgacaat actccttcag tctcacttgt gatgaaagac agctttcctt acctggagcc 2641 TCTGGGTGCA ATTCCAGATG TGCAGAAAAA GATGCTGACT GCTTATGATC TGATGATTCA 2701 AGAGAGCCGG TTTCTCATAG AAATGGCGGA CACAGTCCAG GAAAAGATTG TACAGTGTCA 2761 GAAAGCAGGG ATGGAGTTCC ATGAAGAACT TCATAATTTG GGGGCAAAAG AAGGCTTGAA 2821 GGGAAGAAAA CTCAACAAAG CAACTGAGAG CTTTGCTTGG AACATTACAG TATTGAAGGG 2881 CCAAGGAGAT CTGTTGAAGA ATGCCAAGAA TGAAGCTATA GAAAACATGA AGCAGATCCA 2941 GCTGGCATGC CTGTCCTGTG GACTGAGTAA AGCCCCCAGC AGCAGTGCTG AGGCCAAGAG 3001 CAAGCGCAGC CTGGAAGCCA TAGAGGAGAA GGAAAGTAGT GAGGAGAATG GGAAGCTGTT 3061 GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG 3121 TCTCGATTCT ACGTAGTAAT GAACTAGTCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 3181 TATATATCTT GTGGAAAGGA CGACGGGTAT ATCCCGATTC TAAAAACACG CGTTAAGTCg 3241 acaatcaacc tctggattac aaaatttgtg aaagatt