Construct: ORF TRCN0000477687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007859.1_s317c1
- Derived from:
- BRDN0000391341
- DNA Barcode:
- CTACCTGCTTTGCGTCGACTGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNH1 (3756)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477687
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3756 | KCNH1 | potassium voltage-gated cha... | NM_002238.4 | 99.6% | 99.5% | (many diffs) |
| 2 | human | 3756 | KCNH1 | potassium voltage-gated cha... | NM_172362.3 | 96.9% | 96.8% | (many diffs) |
| 3 | human | 3756 | KCNH1 | potassium voltage-gated cha... | XM_017001246.1 | 61.8% | 61.7% | (many diffs) |
| 4 | mouse | 16510 | Kcnh1 | potassium voltage-gated cha... | NM_001038607.2 | 88.2% | 96.7% | (many diffs) |
| 5 | mouse | 16510 | Kcnh1 | potassium voltage-gated cha... | NM_010600.3 | 85.8% | 94.1% | (many diffs) |
| 6 | mouse | 16510 | Kcnh1 | potassium voltage-gated cha... | XM_006497229.1 | 80.4% | 88.1% | (many diffs) |
| 7 | mouse | 16510 | Kcnh1 | potassium voltage-gated cha... | XR_373887.3 | 50.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2955
- ORF length:
- 2886
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccatggct gggggcgata ggggactagt ggcccctcaa aacacgtttc 121 tggagaatat tgttcggcgg tccaatgata ctaattttgt gttggggaat gctcagatag 181 tggactggcc tattgtgtac agcaatgatg gattttgcaa gctgtctggc tatcacaggg 241 cagaagtgat gcaaaaaagc agcacctgca gttttatgta tggggagctg actgataaag 301 acacgattga aaaagtgcgg caaacatttg agaactatga gatgaattcc tttgaaattc 361 tgatgtacaa gaagaacagg acacctgtgt ggttctttgt gaaaattgct ccaattcgaa 421 acgaacagga taaagtggtt ttatttcttt gcactttcag tgacataaca gctttcaaac 481 agccaattga ggatgattca tgtaaaggct gggggaagtt tgctcggctg acaagagcac 541 tgacaagcag caggggtgtc ctgcagcagc tggctccaag cgtgcaaaaa ggcgagaatg 601 tccacaagca ctcccgcctg gcagaggtcc tacagctggg ctcagacatc cttccccagt 661 acaagcaaga ggcaccaaag actccccctc acatcatctt acattattgt gtttttaaga 721 ccacgtggga ttggatcatc ttgatcttga ccttctatac agccatcttg gtcccttata 781 atgtctcctt caaaaccagg cagaataatg tggcctggct ggttgttgat agcatcgtgg 841 atgttatctt tttggtggac attgtgctca attttcatac cacctttgtt ggaccagcag 901 gggaggtgat ttctgacccc aaacttatcc gcatgaacta cctgaagacg tggtttgtga 961 ttgaccttct gtcctgtttg ccatatgatg tcatcaacgc ttttgagaac gtggatgagg 1021 gcatcagcag cctgttcagc tctctaaaag ttgtccggct gctccgtctt gggcgagtgg 1081 cccgtaagct ggaccactac attgaatatg gagctgctgt gctggtcctg ctggtgtgtg 1141 tgtttgggct ggctgcacac tggatggcct gcatctggta cagcattggg gactatgaga 1201 tctttgacga ggacaccaag acaatccgca acaacagctg gctgtaccaa ctagcgatgg 1261 acattggcac cccttaccag tttaatgggt ctggctcagg gaagtgggaa ggtggtccca 1321 gcaagaattc tgtctacatc tcctcgttgt atttcacaat gaccagcctc accagtgtgg 1381 gctttgggaa catcgcccca tccacagaca ttgagaagat ctttgcagtg gccatcatga 1441 tgattggctc acttctctat gccaccatct tcgggaatgt gacgactatt ttccaacaga 1501 tgtatgccaa caccaacaga taccatgaga tgctcaacag tgttcgggac ttcctgaagc 1561 tctaccaggt gccaaaagga ttgagtgagc gagtaatgga ttatattgtg tccacttggt 1621 ccatgtccag aggcattgac acagagaagg tcctgcagat ctgccccaag gacatgagag 1681 ccgacatctg cgtgcacctg aaccgcaagg tgttcaagga gcacccggcc ttccggctgg 1741 ccagtgatgg ctgcctccgg gcactggcca tggagttcca gacggtgcac tgtgccccag 1801 gggacctcat ctaccatgca ggagagagcg ttgacagcct ctgctttgtg gtttctggct 1861 ccctggaggt gatccaagat gatgaggtgg tggccattct aggaaaagga gacgtgtttg 1921 gagatgtgtt ctggaaggaa gccacccttg cccagtcctg tgccaatgtt agggccttga 1981 cctactgtga tctgcatgtg atcaagcggg atgccctgca gaaagtgctg gaattctaca 2041 cggccttctc ccattccttc tcccggaacc tgattctgac gtacaacttg aggaagagga 2101 ttgtgttccg gaagatcagc gacgtgaaac gtgaagagga agaacgcatg aaacgaaaga 2161 atgaggcccc cctgatcttg cccccggacc accctgtccg gcgcctcttc cagagattcc 2221 gacagcagaa agaggccagg ctggcagctg agagaggggg ccgggacctg gatgacctag 2281 atgtggagaa gggcaatgtc cttacagagc atgcctccgc caaccacagc ctcgtgaagg 2341 ccagcgtggt caccgtgcgt gagagtcctg ccacgcccgt atccttccag gcagcctcca 2401 cctccggggt gccagaccac gcaaagctac aggcgccagg gtccgagtgc ctgggcccca 2461 antngnttgg gggcgattgt gccaagcgca aaagctgggc ccgcttcaaa gatgcttgcg 2521 ggaagagtga ggactggaac aaggtgtcca aggctgagtc gatGGAGACA CTTCCCGAGA 2581 GGACAAAAGC GTCAGGCGAG GCCACACTGA AGAAGACAGA CTCGTGTGAC AGTGGCATCA 2641 CCAAGAGCGA CTTGCGCCTG GACAACGTGG GTGAGGCCAG GAGTCCCCAG GATCGGAGTC 2701 CCATCCTGGC AGAGGTCAAG CATTCGTTCT ACCCCATCCC TGAGCAGACG CTGCAGGCCA 2761 CAGTCCTGGA GGTGAGGCAC GAGCTGAAGG AGGACATCAA GGCCTTAAAC GCCAAAATGA 2821 CCAATATTGA GAAACAGCTC TCTGAGATAC TCAGGATATT AACTTCCAGA AGATCCTCTC 2881 AGTCTCCTCA GGAGTTGTTT GAAATATCGA GGCCACAGTC CCCAGAATCA GAGAGAGACA 2941 TTTTTGGAGC CAGCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 3001 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 3061 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTACCTGCTT TGCGTCGACT 3121 GAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt