Construct: ORF TRCN0000477687

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007859.1_s317c1
Derived from:
BRDN0000391341
DNA Barcode:
CTACCTGCTTTGCGTCGACTGAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KCNH1 (3756)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477687
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3756 KCNH1 potassium voltage-gated cha... NM_002238.4 99.6% 99.5% (many diffs)
2 human 3756 KCNH1 potassium voltage-gated cha... NM_172362.3 96.9% 96.8% (many diffs)
3 human 3756 KCNH1 potassium voltage-gated cha... XM_017001246.1 61.8% 61.7% (many diffs)
4 mouse 16510 Kcnh1 potassium voltage-gated cha... NM_001038607.2 88.2% 96.7% (many diffs)
5 mouse 16510 Kcnh1 potassium voltage-gated cha... NM_010600.3 85.8% 94.1% (many diffs)
6 mouse 16510 Kcnh1 potassium voltage-gated cha... XM_006497229.1 80.4% 88.1% (many diffs)
7 mouse 16510 Kcnh1 potassium voltage-gated cha... XR_373887.3 50.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2955
ORF length:
2886
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaccatggct gggggcgata ggggactagt ggcccctcaa aacacgtttc
121tggagaatat tgttcggcgg tccaatgata ctaattttgt gttggggaat gctcagatag
181tggactggcc tattgtgtac agcaatgatg gattttgcaa gctgtctggc tatcacaggg
241cagaagtgat gcaaaaaagc agcacctgca gttttatgta tggggagctg actgataaag
301acacgattga aaaagtgcgg caaacatttg agaactatga gatgaattcc tttgaaattc
361tgatgtacaa gaagaacagg acacctgtgt ggttctttgt gaaaattgct ccaattcgaa
421acgaacagga taaagtggtt ttatttcttt gcactttcag tgacataaca gctttcaaac
481agccaattga ggatgattca tgtaaaggct gggggaagtt tgctcggctg acaagagcac
541tgacaagcag caggggtgtc ctgcagcagc tggctccaag cgtgcaaaaa ggcgagaatg
601tccacaagca ctcccgcctg gcagaggtcc tacagctggg ctcagacatc cttccccagt
661acaagcaaga ggcaccaaag actccccctc acatcatctt acattattgt gtttttaaga
721ccacgtggga ttggatcatc ttgatcttga ccttctatac agccatcttg gtcccttata
781atgtctcctt caaaaccagg cagaataatg tggcctggct ggttgttgat agcatcgtgg
841atgttatctt tttggtggac attgtgctca attttcatac cacctttgtt ggaccagcag
901gggaggtgat ttctgacccc aaacttatcc gcatgaacta cctgaagacg tggtttgtga
961ttgaccttct gtcctgtttg ccatatgatg tcatcaacgc ttttgagaac gtggatgagg
1021gcatcagcag cctgttcagc tctctaaaag ttgtccggct gctccgtctt gggcgagtgg
1081cccgtaagct ggaccactac attgaatatg gagctgctgt gctggtcctg ctggtgtgtg
1141tgtttgggct ggctgcacac tggatggcct gcatctggta cagcattggg gactatgaga
1201tctttgacga ggacaccaag acaatccgca acaacagctg gctgtaccaa ctagcgatgg
1261acattggcac cccttaccag tttaatgggt ctggctcagg gaagtgggaa ggtggtccca
1321gcaagaattc tgtctacatc tcctcgttgt atttcacaat gaccagcctc accagtgtgg
1381gctttgggaa catcgcccca tccacagaca ttgagaagat ctttgcagtg gccatcatga
1441tgattggctc acttctctat gccaccatct tcgggaatgt gacgactatt ttccaacaga
1501tgtatgccaa caccaacaga taccatgaga tgctcaacag tgttcgggac ttcctgaagc
1561tctaccaggt gccaaaagga ttgagtgagc gagtaatgga ttatattgtg tccacttggt
1621ccatgtccag aggcattgac acagagaagg tcctgcagat ctgccccaag gacatgagag
1681ccgacatctg cgtgcacctg aaccgcaagg tgttcaagga gcacccggcc ttccggctgg
1741ccagtgatgg ctgcctccgg gcactggcca tggagttcca gacggtgcac tgtgccccag
1801gggacctcat ctaccatgca ggagagagcg ttgacagcct ctgctttgtg gtttctggct
1861ccctggaggt gatccaagat gatgaggtgg tggccattct aggaaaagga gacgtgtttg
1921gagatgtgtt ctggaaggaa gccacccttg cccagtcctg tgccaatgtt agggccttga
1981cctactgtga tctgcatgtg atcaagcggg atgccctgca gaaagtgctg gaattctaca
2041cggccttctc ccattccttc tcccggaacc tgattctgac gtacaacttg aggaagagga
2101ttgtgttccg gaagatcagc gacgtgaaac gtgaagagga agaacgcatg aaacgaaaga
2161atgaggcccc cctgatcttg cccccggacc accctgtccg gcgcctcttc cagagattcc
2221gacagcagaa agaggccagg ctggcagctg agagaggggg ccgggacctg gatgacctag
2281atgtggagaa gggcaatgtc cttacagagc atgcctccgc caaccacagc ctcgtgaagg
2341ccagcgtggt caccgtgcgt gagagtcctg ccacgcccgt atccttccag gcagcctcca
2401cctccggggt gccagaccac gcaaagctac aggcgccagg gtccgagtgc ctgggcccca
2461antngnttgg gggcgattgt gccaagcgca aaagctgggc ccgcttcaaa gatgcttgcg
2521ggaagagtga ggactggaac aaggtgtcca aggctgagtc gatGGAGACA CTTCCCGAGA
2581GGACAAAAGC GTCAGGCGAG GCCACACTGA AGAAGACAGA CTCGTGTGAC AGTGGCATCA
2641CCAAGAGCGA CTTGCGCCTG GACAACGTGG GTGAGGCCAG GAGTCCCCAG GATCGGAGTC
2701CCATCCTGGC AGAGGTCAAG CATTCGTTCT ACCCCATCCC TGAGCAGACG CTGCAGGCCA
2761CAGTCCTGGA GGTGAGGCAC GAGCTGAAGG AGGACATCAA GGCCTTAAAC GCCAAAATGA
2821CCAATATTGA GAAACAGCTC TCTGAGATAC TCAGGATATT AACTTCCAGA AGATCCTCTC
2881AGTCTCCTCA GGAGTTGTTT GAAATATCGA GGCCACAGTC CCCAGAATCA GAGAGAGACA
2941TTTTTGGAGC CAGCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
3001CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
3061TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTACCTGCTT TGCGTCGACT
3121GAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)

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