Construct: ORF TRCN0000477744
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012893.1_s317c1
- Derived from:
- ccsbBroadEn_10634
- DNA Barcode:
- AAGCGGTCACCAGAATGAGGGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC407835 (407835)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477744
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5605 | MAP2K2 | mitogen-activated protein k... | NM_030662.3 | 90.9% | 88% | (many diffs) |
| 2 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026990.1 | 69.8% | 48.8% | (many diffs) |
| 3 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026989.1 | 69.7% | 64.2% | (many diffs) |
| 4 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_006722799.2 | 69.5% | 68.2% | (many diffs) |
| 5 | human | 407835 | LOC407835 | mitogen-activated protein k... | NR_002144.1 | 65.5% | 1_247del;926G>A;1388_1737del | |
| 6 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026991.1 | 62.3% | 60.3% | (many diffs) |
| 7 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | NM_001347144.1 | 81.6% | 85% | (many diffs) |
| 8 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | NM_023138.5 | 81.4% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1209
- ORF length:
- 1140
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctggcccgg aggaagccga tgctgccggc gctcaccatc aaccctacca 121 tcgccgaggg cccgtcccca accagcgagg gcgcctccga ggcaaacctg gtggacctgc 181 agaagaagct ggaggagctg gaacttgacg agcagcagaa gcggctggaa gcctttctca 241 cccagaaagc caaggtcggc gaactcaaag acgatgactt cgaaaggacc tcagagctgg 301 acgcgggcaa cggcggggtg gtcaccaaag tccagcacag accctcgggc ctcatcatgg 361 ccaggaagct gatccacctt gagatcaagc cggccatccg gaaccagatc atccgcgagc 421 accaggtcct gcacgagtgc aactcaccgt acatcgtggg cttctacggg gccttctact 481 gtgacaggga gatcagcatc tgcatggagc acatggatgg cggctccctg gaccaggggc 541 tgaaagaggc caagaggatt cccgaggaca tcctggggaa agtcagcatt gcggttctcc 601 ggggcttggc gtacctccga gagaagcacc agatcatgca ccgaaatgtg aagccctcca 661 acatcctcgt gaactctaga ggggagatca agctgtgtga cttcggggtg agcggccagc 721 tcatcgactc catggccaac tccttcatgg gcacgcgctc ctacatggct ccggagcggt 781 tgcagggcac acattactcg gtgcagtcgg tcatctggag catggacctg tccctggtgg 841 agctggccat cgaaaggtac cccatccccc cgcccgacgc caaggagctg gaggccaTCT 901 TTGGCCAGCC CGTGGTCGAC AGGGAAGAAG GAGAGCCTCA CAGCATCTCC TCTTGGCCAG 961 GGTCCCCCGG GCGCCCCAAC AGCGGTTACG GGATGGACAG CCTGCCCGCC ATGGCCATCT 1021 TCGAACTGCT GGACTATATT GTGAAAGAGC CGCCTCCTAA GCTGCCCAAC GGTGTGTTCA 1081 CCCCCGACTT CCAGGAGTTT GTCAATAAAT GCCTCATCAA AAACCCAACG GAGCGGGCGG 1141 ACCTAAAGAT GCTCACAAAC CACGCCTTCA TCAAGCGGTC CGAGGTGAAA GAAGCGGATT 1201 TTGCCTGCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1261 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1321 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAGCGG TCACCAGAAT GAGGGTTCAC 1381 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt