Construct: ORF TRCN0000477773
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005358.1_s317c1
- Derived from:
- ccsbBroadEn_13631
- DNA Barcode:
- GCACCGACAGTTTAATGATTATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC375010 (375010)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477773
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100874392 | ANKRD20A12P | ankyrin repeat domain 20 fa... | NR_046228.1 | 9% | 1_160del;225T>C;320_1744del | |
| 2 | human | 441425 | ANKRD20A3 | ankyrin repeat domain 20 fa... | XM_005272525.4 | 5.9% | 4.8% | (many diffs) |
| 3 | human | 441430 | ANKRD20A2 | ankyrin repeat domain 20 fa... | XM_024447553.1 | 5.9% | 4.8% | (many diffs) |
| 4 | human | 441425 | ANKRD20A3 | ankyrin repeat domain 20 fa... | NM_001012419.2 | 5.9% | 4.8% | (many diffs) |
| 5 | human | 441430 | ANKRD20A2 | ankyrin repeat domain 20 fa... | NM_001012421.1 | 5.9% | 4.8% | (many diffs) |
| 6 | human | 728747 | ANKRD20A4 | ankyrin repeat domain 20 fa... | NM_001098805.1 | 5.8% | 4.8% | (many diffs) |
| 7 | human | 84210 | ANKRD20A1 | ankyrin repeat domain 20 fa... | NM_032250.3 | 5.8% | 4.7% | (many diffs) |
| 8 | human | 441430 | ANKRD20A2 | ankyrin repeat domain 20 fa... | XM_011517878.2 | 5.8% | 4.8% | (many diffs) |
| 9 | human | 441425 | ANKRD20A3 | ankyrin repeat domain 20 fa... | XM_011543860.3 | 5.8% | 4.8% | (many diffs) |
| 10 | human | 441430 | ANKRD20A2 | ankyrin repeat domain 20 fa... | XM_011517877.3 | 5.8% | 4.7% | (many diffs) |
| 11 | human | 102723552 | ANKRD20A21P | ankyrin repeat domain 20 fa... | XR_002958558.1 | 4.9% | (many diffs) | |
| 12 | human | 284232 | ANKRD20A9P | ankyrin repeat domain 20 fa... | NR_138091.1 | 4.5% | (many diffs) | |
| 13 | human | 440482 | ANKRD20A5P | ankyrin repeat domain 20 fa... | NR_040113.1 | 4% | (many diffs) | |
| 14 | human | 729171 | ANKRD20A8P | ankyrin repeat domain 20 fa... | NR_003366.2 | 3.9% | (many diffs) | |
| 15 | human | 105379426 | LOC105379426 | ankyrin repeat domain-conta... | XR_001756105.1 | 3.8% | (many diffs) | |
| 16 | human | 110006327 | ANKRD20A4-ANKRD20A20P | ANKRD20A4-ANKRD20A20P readt... | NR_146419.1 | 3.3% | (many diffs) | |
| 17 | human | 441425 | ANKRD20A3 | ankyrin repeat domain 20 fa... | XR_001746296.2 | 3.1% | (many diffs) | |
| 18 | human | 441425 | ANKRD20A3 | ankyrin repeat domain 20 fa... | XR_001746295.2 | 3.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 218
- ORF end:
- 377
- ORF length:
- 159
- Sequence:
-
1 TTTTATGCGA TGGAGTTTCC CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG 61 CACTTGATGT AATTCTCCTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC 121 AAGCCTCAGA CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGCTAGCAT 181 CGATTGATCA ACAAGTTTGT ACAAAAAAGT TGGCACCATG AGTTCTGATA TTTCCGAAGT 241 AGAAGATAAG AATGAGTTTC TTACTGAACA ACTTTCTAAA ACGCAAATTA AACTCAATAC 301 CTTAAAAGAT AAGTTCCGTA AGAAAAGAGA TACTCTCAGA AAAAAGTCAT CGGCTTTAGA 361 AACTCTCCAA ACGACCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 421 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 481 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCACCGAC AGTTTAATGA 541 TTATCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt