Construct: ORF TRCN0000477850
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011472.1_s317c1
- Derived from:
- ccsbBroadEn_01631
- DNA Barcode:
- TACATCGAAATTTTCCAAGAGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYCP1 (6847)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477850
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6847 | SYCP1 | synaptonemal complex protein 1 | NM_001282541.1 | 100% | 100% | |
| 2 | human | 6847 | SYCP1 | synaptonemal complex protein 1 | NM_003176.4 | 100% | 100% | |
| 3 | human | 6847 | SYCP1 | synaptonemal complex protein 1 | XM_006710859.1 | 100% | 100% | |
| 4 | human | 6847 | SYCP1 | synaptonemal complex protein 1 | XM_017002184.1 | 100% | 100% | |
| 5 | human | 6847 | SYCP1 | synaptonemal complex protein 1 | XM_005271155.4 | 98.1% | 98.1% | 235_236ins54 |
| 6 | human | 6847 | SYCP1 | synaptonemal complex protein 1 | NM_001282542.1 | 97.4% | 97.4% | 2246_2247ins75 |
| 7 | human | 6847 | SYCP1 | synaptonemal complex protein 1 | XM_005271156.1 | 94.3% | 94.3% | 291_292ins165 |
| 8 | human | 6847 | SYCP1 | synaptonemal complex protein 1 | NR_104211.2 | 82.1% | 1_180del;2884_2986del;3212_3565del | |
| 9 | human | 6847 | SYCP1 | synaptonemal complex protein 1 | XM_017002185.1 | 80.7% | 79.9% | (many diffs) |
| 10 | human | 6847 | SYCP1 | synaptonemal complex protein 1 | XM_011542037.2 | 80.1% | 79% | (many diffs) |
| 11 | human | 6847 | SYCP1 | synaptonemal complex protein 1 | XM_011542038.2 | 78.3% | 78.3% | 0_1ins633 |
| 12 | human | 6847 | SYCP1 | synaptonemal complex protein 1 | XM_011542039.1 | 56.3% | 56.2% | 1650_1650delAins1279 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2997
- ORF length:
- 2928
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaaagcaa aagccctttg cattgttcgt accaccgaga tcaagcagca 121 gtcaggtgtc tgcggtgaaa cctcagaccc tgggaggcga ttccactttc ttcaagagtt 181 tcaacaaatg tactgaagat gattttgagt ttccatttgc aaagactaat ctctccaaaa 241 atggggaaaa cattgattca gatcctgctt tacaaaaagt taatttcttg cccgtgcttg 301 agcaggttgg taattctgac tgtcactatc aggaaggact aaaagactct gatttggaga 361 attcagaggg attgagcaga gtgtattcaa aactgtataa ggaggctgaa aagataaaaa 421 aatggaaagt aagtacagaa gctgaactga gacagaaaga aagtaagttg caagaaaaca 481 gaaagataat tgaagcacag cgaaaagcca ttcaggaact gcaatttgga aatgaaaaag 541 taagtttgaa attagaagaa ggaatacaag aaaataaaga tttaataaaa gagaataatg 601 ccacaaggca tttatgtaat ctactcaaag aaacctgtgc tagatctgca gaaaagacaa 661 agaaatatga atatgaacgg gaagaaacca ggcaagttta tatggatcta aataataaca 721 ttgagaaaat gataacagct tttgaggaac ttcgtgtgca agctgagaat tccagactgg 781 aaatgcattt taagttaaag gaagattatg aaaaaatcca acaccttgaa caagaataca 841 agaaggaaat aaatgacaag gaaaagcagg tatcactact attgatccaa atcactgaga 901 aagaaaataa aatgaaagat ttaacatttc tgctagagga atccagagat aaagttaatc 961 aattagagga aaagacaaaa ttacagagtg aaaacttaaa acaatcaatt gagaaacagc 1021 atcatttgac taaagaacta gaagatatta aagtgtcatt acaaagaagt gtgagtactc 1081 aaaaggcttt agaggaagat ttacagatag caacaaaaac aatttgtcag ctaactgaag 1141 aaaaagaaac tcaaatggaa gaatctaata aagctagagc tgctcattcg tttgtggtta 1201 ctgaatttga aactactgtc tgcagcttgg aagaattatt gagaacagaa cagcaaagat 1261 tggaaaaaaa tgaagatcaa ttgaaaatac ttaccatgga gcttcaaaag aaatcaagtg 1321 agctggaaga gatgactaag cttacaaata acaaagaagt agaacttgaa gaattgaaaa 1381 aagtcttggg agaaaaggaa acacttttat atgaaaataa acaatttgag aagattgctg 1441 aagaattaaa aggaacagaa caagaactaa ttggtcttct ccaagccaga gagaaagaag 1501 tacatgattt ggaaatacag ttaactgcca ttaccacaag tgaacagtat tattcaaaag 1561 aggttaaaga tctaaaaact gagcttgaaa acgagaagct taagaatact gaattaactt 1621 cacactgcaa caagctttca ctagaaaaca aagagctcac acaggaaaca agtgatatga 1681 ccctagaact caagaatcag caagaagata ttaataataa caaaaagcaa gaagaaagga 1741 tgttgaaaca aatagaaaat cttcaagaaa cagaaaccca attaagaaat gaactagaat 1801 atgtgagaga agagctaaaa cagaaaagag atgaagttaa atgtaaattg gacaagagtg 1861 aagaaaattg taacaattta aggaaacaag ttgaaaataa aaacaagtat attgaagaac 1921 ttcagcagga gaataaggcc ttgaaaaaaa aaggtacagc agaaagcaag caactgaatg 1981 tttatgagat aaaggtcaat aaattagagt tagaactaga aagtgccaaa cagaaatttg 2041 gagaaatcac agacacctat cagaaagaaa ttgaggacaa aaagatatca gaagaaaatc 2101 ttttggaaga ggttgagaaa gcaaaagtaa tagctgatga agcagtaaaa ttacagaaag 2161 aaattgataa gcgatgtcaa cataaaatag ctgaaatggt agcacttatg gaaaaacata 2221 agcaccaata tgataagatc attgaagaaa gagactcaga attaggactt tataagagca 2281 aagaacaaga acagtcatca ctgagagcat ctttggagat tgaactatcc aatctcaaag 2341 ctgaactttt gtctgttaag aagcaacttg aaatagaaag agaagagaag gaaaaactca 2401 aaagagaggc aaaagaaaac acagctactc ttaaagaaaa aaaagacaag aaaacacaaa 2461 catttttatt ggaaacacct gaaatttatt ggaaattgga ttctaaagca gttccttcaC 2521 AAACTGTATC TCGAAATTTC ACATCAGTTG ATCATGGCAT ATCCAAAGAT AAAAGAGACT 2581 ATCTGTGGAC ATCTGCCAAA AATACTTTAT CTACACCATT GCCAAAGGCA TATACAGTGA 2641 AGACACCAAC AAAACCAAAA CTACAGCAAA GAGAAAACTT GAATATACCC ATTGAAGAAA 2701 GTAAAAAAAA GAGAAAAATG GCCTTTGAAT TTGATATTAA TTCAGATAGT TCAGAAACTA 2761 CTGATCTTTT GAGCATGGTT TCAGAAGAAG AGACATTGAA AACACTGTAT AGGAACAATA 2821 ATCCACCAGC TTCTCATCTT TGTGTCAAAA CACCAAAAAA GGCCCCTTCA TCTCTAACAA 2881 CCCCTGGATC TACACTGAAG TTTGGAGCTA TAAGAAAAAT GCGGGAGGAC CGTTGGGCTG 2941 TAATTGCTAA AATGGATAGA AAAAAAAAAC TAAAAGAAGC TGAAAAGTTA TTTGTTTTGC 3001 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 3061 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 3121 ATATATCTTG TGGAAAGGAC GATACATCGA AATTTTCCAA GAGGCCACGC GTTAAGTCga 3181 caatcaacct ctggattaca aaatttgtga aagatt