Construct: ORF TRCN0000477873

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013351.1_s317c1
Derived from:
ccsbBroadEn_13173
DNA Barcode:
CAGCACTTATTCCGCAATTATGCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CPEB2 (132864)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477873
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 132864 CPEB2 cytoplasmic polyadenylation... XM_017007733.1 74.9% 74.9% 0_1ins345;231_320del
2 human 132864 CPEB2 cytoplasmic polyadenylation... XM_017007734.2 74.9% 74.9% 0_1ins345;231_320del
3 human 132864 CPEB2 cytoplasmic polyadenylation... NM_001177381.2 54.4% 54.4% 1_1311del;1435_1500del
4 human 132864 CPEB2 cytoplasmic polyadenylation... NM_001177383.2 54.1% 54.1% 1_1311del;1435_1500del;1943_1944insGGTAAGAAT
5 human 132864 CPEB2 cytoplasmic polyadenylation... NM_001177384.2 53.6% 53.6% 1_1311del;1435_1500del;2094_2095ins24
6 human 132864 CPEB2 cytoplasmic polyadenylation... NM_182646.3 53.3% 53.3% (many diffs)
7 human 132864 CPEB2 cytoplasmic polyadenylation... NM_001177382.2 52.9% 52.9% (many diffs)
8 human 132864 CPEB2 cytoplasmic polyadenylation... XM_005248135.3 52.8% 52.8% 1_1311del;1435_1500del;1953_2042del
9 human 132864 CPEB2 cytoplasmic polyadenylation... NM_182485.3 52.1% 52.1% (many diffs)
10 human 132864 CPEB2 cytoplasmic polyadenylation... XM_011513777.3 52% 52% (many diffs)
11 human 80315 CPEB4 cytoplasmic polyadenylation... NM_001308193.1 46.4% 53.2% (many diffs)
12 human 22849 CPEB3 cytoplasmic polyadenylation... XM_017015927.1 45% 50% (many diffs)
13 human 132864 CPEB2 cytoplasmic polyadenylation... XM_011513778.3 22.4% 21.5% (many diffs)
14 human 132864 CPEB2 cytoplasmic polyadenylation... XM_011513779.2 20.1% 18.8% (many diffs)
15 mouse 231207 Cpeb2 cytoplasmic polyadenylation... XM_006503930.3 51.1% 54.2% (many diffs)
16 mouse 231207 Cpeb2 cytoplasmic polyadenylation... NM_001177379.1 50.8% 53.9% (many diffs)
17 mouse 231207 Cpeb2 cytoplasmic polyadenylation... XM_006503931.2 50.3% 53.4% (many diffs)
18 mouse 231207 Cpeb2 cytoplasmic polyadenylation... XM_006503932.3 50% 53.1% (many diffs)
19 mouse 231207 Cpeb2 cytoplasmic polyadenylation... XM_006503928.2 49.7% 52.6% (many diffs)
20 mouse 231207 Cpeb2 cytoplasmic polyadenylation... XM_006503927.2 49.6% 52.6% (many diffs)
21 mouse 231207 Cpeb2 cytoplasmic polyadenylation... NM_175937.3 48.9% 51.9% (many diffs)
22 mouse 231207 Cpeb2 cytoplasmic polyadenylation... XM_006503929.2 48.8% 51.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1713
ORF length:
1644
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gccgccgccc agccccgact cagagaacgg cttctacccc gggctgccgt
121cgtccatgaa cccggccttc ttccctagct tctcgcccgt gtcgccgcac ggctgcactg
181ggctcagcgt tccgccgccc gccatgaata tacctcaaca gcagcccccg ccgcccgcgg
241cgccgcagca gccgcagagc cggaggtcgc ccgtcagccc gcagctccag cagcagcacc
301aggcggcggc cgccgccttc ctgcagcaga ggaactccta taaccaccac cagcctcttc
361tgaaacagtc tccctggagc aaccatcaga gcagtggctg gggcactgga agtatgtcct
421ggggagcaat gcatggcaga gatcatcgta gaaccggaaa catgggaatc ccaggaacta
481tgaatcagat atctccattg aagaaaccgt tttctggtaa tgtcatagca ccaccgaaat
541ttactcgctc aactccatca ctgactccaa aatcttggat tgaagataat gtgttcagaa
601cagacaacaa tagtaataca ctcttaccct tacaggtaag aatggatcga agtagaatgt
661atgacagttt gaatatgcac tctttggaaa attcccttat cgatattatg agagcagagc
721atgatcctct taagggtcga ttgagctatc cacatccagg aactgacaat ctgttgatgt
781taaatgcaag gagttatggg cgaagacgag gtcgttcttc cctctttcca atagatgatg
841gcttgcttga tgatggtcac agtgatcaag ttggagtttt aaattcaccc acctgttatt
901cagctcacca aaatggagag cgaatagaac gcttctctcg aaaagttttt gttggtggtc
961ttcctccaga tattgatgaa gatgaaataa ctgctagctt cagaagattt gggcctttgg
1021tagtagattg gcctcataaa gcagaaagca agtcctattt tccaccaaaa ggctatgcat
1081ttcttctctt tcaagaagag agctcagttc aggcactcat tgatgcttgt attgaagaag
1141atggaaaact ctatctgtgt gtttctagcc ctactatcaa ggacaaacca gttcaaatac
1201gtccttggaa tttaagtgat agtgattttg taatggatgg ttctcagcct ttggatcccc
1261gaaaaacaat ttttgttgga ggtgttccta ggccattaag ggctgtggaa cttgctatga
1321tcatggaccg gctgtatggt ggagtttgtt atgcaggaat tgatacagat ccTGAGCTAA
1381AATACCCAAA AGGTGCTGGG CGAGTTGCTT TCTCCAATCA GCAGAGCTAT ATTGCTGCCA
1441TTAGTGCTCG GTTTGTTCAG CTTCAGCATG GTGATATTGA TAAACGTGTG GAGGTAAAGC
1501CATATGTGCT AGATGACCAG ATGTGTGATG AATGCCAGGG CGCACGCTGT GGTGGAAAAT
1561TTGCTCCCTT TTTTTGTGCC AATGTCACTT GCCTGCAGTA TTACTGTGAG TTTTGTTGGG
1621CAAATATCCA CTCTCGTGCT GGACGTGAGT TCCATAAGCC ATTGGTAAAG GAAGGTGCTG
1681ATCGCCCACG TCAGATCCAC TTCCGCTGGA ACTTGCCAAC TTTCTTGTAC AAAGTGGTTG
1741ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1801GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA
1861GCACTTATTC CGCAATTATG CGACGCGTTA AGTCgacaat caacctctgg attacaaaat
1921ttgtgaaaga tt

Download FASTA (ORF) (Full)