Construct: ORF TRCN0000477873
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013351.1_s317c1
- Derived from:
- ccsbBroadEn_13173
- DNA Barcode:
- CAGCACTTATTCCGCAATTATGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPEB2 (132864)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477873
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 132864 | CPEB2 | cytoplasmic polyadenylation... | XM_017007733.1 | 74.9% | 74.9% | 0_1ins345;231_320del |
| 2 | human | 132864 | CPEB2 | cytoplasmic polyadenylation... | XM_017007734.2 | 74.9% | 74.9% | 0_1ins345;231_320del |
| 3 | human | 132864 | CPEB2 | cytoplasmic polyadenylation... | NM_001177381.2 | 54.4% | 54.4% | 1_1311del;1435_1500del |
| 4 | human | 132864 | CPEB2 | cytoplasmic polyadenylation... | NM_001177383.2 | 54.1% | 54.1% | 1_1311del;1435_1500del;1943_1944insGGTAAGAAT |
| 5 | human | 132864 | CPEB2 | cytoplasmic polyadenylation... | NM_001177384.2 | 53.6% | 53.6% | 1_1311del;1435_1500del;2094_2095ins24 |
| 6 | human | 132864 | CPEB2 | cytoplasmic polyadenylation... | NM_182646.3 | 53.3% | 53.3% | (many diffs) |
| 7 | human | 132864 | CPEB2 | cytoplasmic polyadenylation... | NM_001177382.2 | 52.9% | 52.9% | (many diffs) |
| 8 | human | 132864 | CPEB2 | cytoplasmic polyadenylation... | XM_005248135.3 | 52.8% | 52.8% | 1_1311del;1435_1500del;1953_2042del |
| 9 | human | 132864 | CPEB2 | cytoplasmic polyadenylation... | NM_182485.3 | 52.1% | 52.1% | (many diffs) |
| 10 | human | 132864 | CPEB2 | cytoplasmic polyadenylation... | XM_011513777.3 | 52% | 52% | (many diffs) |
| 11 | human | 80315 | CPEB4 | cytoplasmic polyadenylation... | NM_001308193.1 | 46.4% | 53.2% | (many diffs) |
| 12 | human | 22849 | CPEB3 | cytoplasmic polyadenylation... | XM_017015927.1 | 45% | 50% | (many diffs) |
| 13 | human | 132864 | CPEB2 | cytoplasmic polyadenylation... | XM_011513778.3 | 22.4% | 21.5% | (many diffs) |
| 14 | human | 132864 | CPEB2 | cytoplasmic polyadenylation... | XM_011513779.2 | 20.1% | 18.8% | (many diffs) |
| 15 | mouse | 231207 | Cpeb2 | cytoplasmic polyadenylation... | XM_006503930.3 | 51.1% | 54.2% | (many diffs) |
| 16 | mouse | 231207 | Cpeb2 | cytoplasmic polyadenylation... | NM_001177379.1 | 50.8% | 53.9% | (many diffs) |
| 17 | mouse | 231207 | Cpeb2 | cytoplasmic polyadenylation... | XM_006503931.2 | 50.3% | 53.4% | (many diffs) |
| 18 | mouse | 231207 | Cpeb2 | cytoplasmic polyadenylation... | XM_006503932.3 | 50% | 53.1% | (many diffs) |
| 19 | mouse | 231207 | Cpeb2 | cytoplasmic polyadenylation... | XM_006503928.2 | 49.7% | 52.6% | (many diffs) |
| 20 | mouse | 231207 | Cpeb2 | cytoplasmic polyadenylation... | XM_006503927.2 | 49.6% | 52.6% | (many diffs) |
| 21 | mouse | 231207 | Cpeb2 | cytoplasmic polyadenylation... | NM_175937.3 | 48.9% | 51.9% | (many diffs) |
| 22 | mouse | 231207 | Cpeb2 | cytoplasmic polyadenylation... | XM_006503929.2 | 48.8% | 51.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1713
- ORF length:
- 1644
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccgccgccc agccccgact cagagaacgg cttctacccc gggctgccgt 121 cgtccatgaa cccggccttc ttccctagct tctcgcccgt gtcgccgcac ggctgcactg 181 ggctcagcgt tccgccgccc gccatgaata tacctcaaca gcagcccccg ccgcccgcgg 241 cgccgcagca gccgcagagc cggaggtcgc ccgtcagccc gcagctccag cagcagcacc 301 aggcggcggc cgccgccttc ctgcagcaga ggaactccta taaccaccac cagcctcttc 361 tgaaacagtc tccctggagc aaccatcaga gcagtggctg gggcactgga agtatgtcct 421 ggggagcaat gcatggcaga gatcatcgta gaaccggaaa catgggaatc ccaggaacta 481 tgaatcagat atctccattg aagaaaccgt tttctggtaa tgtcatagca ccaccgaaat 541 ttactcgctc aactccatca ctgactccaa aatcttggat tgaagataat gtgttcagaa 601 cagacaacaa tagtaataca ctcttaccct tacaggtaag aatggatcga agtagaatgt 661 atgacagttt gaatatgcac tctttggaaa attcccttat cgatattatg agagcagagc 721 atgatcctct taagggtcga ttgagctatc cacatccagg aactgacaat ctgttgatgt 781 taaatgcaag gagttatggg cgaagacgag gtcgttcttc cctctttcca atagatgatg 841 gcttgcttga tgatggtcac agtgatcaag ttggagtttt aaattcaccc acctgttatt 901 cagctcacca aaatggagag cgaatagaac gcttctctcg aaaagttttt gttggtggtc 961 ttcctccaga tattgatgaa gatgaaataa ctgctagctt cagaagattt gggcctttgg 1021 tagtagattg gcctcataaa gcagaaagca agtcctattt tccaccaaaa ggctatgcat 1081 ttcttctctt tcaagaagag agctcagttc aggcactcat tgatgcttgt attgaagaag 1141 atggaaaact ctatctgtgt gtttctagcc ctactatcaa ggacaaacca gttcaaatac 1201 gtccttggaa tttaagtgat agtgattttg taatggatgg ttctcagcct ttggatcccc 1261 gaaaaacaat ttttgttgga ggtgttccta ggccattaag ggctgtggaa cttgctatga 1321 tcatggaccg gctgtatggt ggagtttgtt atgcaggaat tgatacagat ccTGAGCTAA 1381 AATACCCAAA AGGTGCTGGG CGAGTTGCTT TCTCCAATCA GCAGAGCTAT ATTGCTGCCA 1441 TTAGTGCTCG GTTTGTTCAG CTTCAGCATG GTGATATTGA TAAACGTGTG GAGGTAAAGC 1501 CATATGTGCT AGATGACCAG ATGTGTGATG AATGCCAGGG CGCACGCTGT GGTGGAAAAT 1561 TTGCTCCCTT TTTTTGTGCC AATGTCACTT GCCTGCAGTA TTACTGTGAG TTTTGTTGGG 1621 CAAATATCCA CTCTCGTGCT GGACGTGAGT TCCATAAGCC ATTGGTAAAG GAAGGTGCTG 1681 ATCGCCCACG TCAGATCCAC TTCCGCTGGA ACTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1741 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1801 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 1861 GCACTTATTC CGCAATTATG CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1921 ttgtgaaaga tt