Construct: ORF TRCN0000478909
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003385.1_s317c1
- Derived from:
- ccsbBroadEn_07902
- DNA Barcode:
- CATGCCTACAGCTCCCATTTCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIGN (23556)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478909
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23556 | PIGN | phosphatidylinositol glycan... | NM_012327.6 | 99.9% | 99.8% | 685C>G;741C>T |
| 2 | human | 23556 | PIGN | phosphatidylinositol glycan... | NM_176787.5 | 99.9% | 99.8% | 685C>G;741C>T |
| 3 | human | 23556 | PIGN | phosphatidylinositol glycan... | XM_011525896.1 | 97.8% | 97.6% | 685C>G;741C>T;2672_2731del |
| 4 | human | 23556 | PIGN | phosphatidylinositol glycan... | XM_011525898.1 | 97% | 94.3% | (many diffs) |
| 5 | human | 23556 | PIGN | phosphatidylinositol glycan... | XM_011525889.1 | 95.9% | 95.7% | 685C>G;741C>T;2672_2788del |
| 6 | human | 23556 | PIGN | phosphatidylinositol glycan... | XM_011525890.1 | 95.9% | 95.7% | 685C>G;741C>T;2672_2788del |
| 7 | human | 23556 | PIGN | phosphatidylinositol glycan... | XM_011525891.1 | 95.9% | 95.7% | 685C>G;741C>T;2672_2788del |
| 8 | human | 23556 | PIGN | phosphatidylinositol glycan... | XM_011525892.1 | 95.9% | 95.7% | 685C>G;741C>T;2672_2788del |
| 9 | human | 23556 | PIGN | phosphatidylinositol glycan... | XM_011525893.1 | 95.9% | 95.7% | 685C>G;741C>T;2672_2788del |
| 10 | human | 23556 | PIGN | phosphatidylinositol glycan... | XM_011525894.1 | 95.9% | 95.7% | 685C>G;741C>T;2672_2788del |
| 11 | human | 23556 | PIGN | phosphatidylinositol glycan... | XM_011525895.1 | 95.9% | 95.7% | 685C>G;741C>T;2672_2788del |
| 12 | human | 23556 | PIGN | phosphatidylinositol glycan... | XM_017025686.1 | 85.5% | 85.4% | 685C>G;741C>T;1171_1172ins402 |
| 13 | human | 23556 | PIGN | phosphatidylinositol glycan... | XM_017025685.1 | 82% | 81.9% | (many diffs) |
| 14 | mouse | 27392 | Pign | phosphatidylinositol glycan... | NM_013784.3 | 86.4% | 87.5% | (many diffs) |
| 15 | mouse | 27392 | Pign | phosphatidylinositol glycan... | XM_017321076.1 | 86.4% | 87.5% | (many diffs) |
| 16 | mouse | 27392 | Pign | phosphatidylinositol glycan... | XM_006529676.3 | 75.7% | 72.9% | (many diffs) |
| 17 | mouse | 27392 | Pign | phosphatidylinositol glycan... | XM_006529677.3 | 73.7% | 73.4% | (many diffs) |
| 18 | mouse | 27392 | Pign | phosphatidylinositol glycan... | XR_387174.3 | 68.7% | (many diffs) | |
| 19 | mouse | 27392 | Pign | phosphatidylinositol glycan... | XM_006529678.3 | 46% | 46.2% | (many diffs) |
| 20 | mouse | 27392 | Pign | phosphatidylinositol glycan... | XR_387175.3 | 34.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2859
- ORF length:
- 2793
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gctgttcttt actttgggat tgcttataca ttttgtgttc ttcgcctcca 121 tctttgacat ttattttaca tctcctttgg ttcatggaat gactcctcag tttacaccat 181 tgcctcctcc agcgagaaga ttagtgttgt ttgttgctga tggccttcga gcagatgcac 241 tttacgaatt agatgaaaat ggaaactcta gagcaccgtt tattaggaat atcataatgc 301 atgaaggcag ctggggcata tctcatacac gtgtgccaac agaatctcgg ccaggtcatg 361 tagctctgat agctgggttt tatgaagatg tcagtgcagt tgccaaagga tggaaggaaa 421 atcctgtaga gtttgattct ctttttaatg aaagtaaata cacatggagc tggggaagcc 481 cagatatcct gcctatgttt gccaaaggtg ctagtggaga ccacgtttat acatatagtt 541 atgatgctaa aagagaggat tttggtgctc aagatgcaac aaaactggat acgtgggttt 601 ttgataatgt taaggacttc tttcatcatg ccagaaacaa ccagtctttg ttttctaaaa 661 taaatgaaga gaaaatagtt tttttcttac atttattagg aatagataca aacggacatg 721 ctcatcgacc atcctcgaga gactacaagg acaatattaa aaaagttgat gatggagtta 781 aagaaatcgt gtctatgttt aaccatttct atggaaatga tgggaaaaca acatttatct 841 ttacctctga ccatggaatg acagactggg gttcccatgg ggctggtcat ccttcagaga 901 ctttaactcc tttagtcact tggggagctg gaatcaagta tccccaaaga gtatcagctc 961 agcaatttga tgatgcattt ttgaaagagt ggagattgga gaattggaag aggctagatg 1021 tcaatcaggc tgatattgca ccattgatga cttcccttat tggagttccc tttcctctta 1081 actcagtggg aatccttcct gtggattatc ttaacaacac tgatctcttc aaagcagaga 1141 gcatgtttac aaatgcagta cagattcttg aacagttcaa ggtgaaaatg actcagaaga 1201 aagaagttac tttaccattt ttgtttacac catttaaact gctttctgat tccaaacagt 1261 tcaacatttt aagaaaagca agatcttata taaaacacag aaagtttgat gaagtggtct 1321 ccctttgcaa ggagctaatt catcttgcat tgaaaggatt gtcctattat cacacatatg 1381 acagattctt tttgggcgtc aatgttgtta ttggttttgt gggatggata tcttatgcct 1441 ctttgttgat catcaagtct cattccaacc ttataaaagg tgttagtaaa gaagtgaaga 1501 aaccaagcca tctcctgcct tgtagttttg tagctattgg cattttagta gcattttttc 1561 tgctgattca agcctgtccc tggacatatt atgtatatgg tttgttgcca ctgccaatat 1621 ggtatgcggt tctaagagaa tttcaagtta ttcaggacct tgttgtatca gtgttgacct 1681 atcctctgag ccattttgtt gggtacctgt tagcctttac cctgggaatt gaagtattag 1741 ttctcagttt tttctaccgc tatatgctta ccgctggact tactgccttt gcagcttggc 1801 catttctcac tcggctgtgg actcgagcaa agatgacctc actgagttgg actttcttct 1861 ctttgctcct ggcagtgttc ccactgatgc cggttgtagg tcgaaagcca gacatctctc 1921 tagtgatggg tgcaggcttg ctggttcttc tgttatccct gtgtgttgta acatctctca 1981 tgaaaagaaa agatagcttt ataaaggaag agctattggt acatctgtta caggtgctga 2041 gcacagtgct ctccatgtat gttgtgtata gcactcagag tagtctactc aggaagcaag 2101 gactgcctct catgaatcaa attattagct gggcaacatt agcctcttcc ttggttgtgc 2161 cactactgag ttctccagtt ctctttcagc gattgttcag catacttctt tcattgatgt 2221 caacctacct acttctaagc acagggtatg aagctctctt tccactagtg ttgtcttgtt 2281 tgatgtttgt ctggataaac atagaacaag aaactctaca acaatctggt gtttgctgta 2341 aacaaaagct caccagtatc cagttctctt ataatactga tataactcag tttcgacagc 2401 tatatctgga tgacatccgt agggcctttt tccttgtttt cttcttagtg acagcatttt 2461 ttggaactgg aaatatagct tctattaaca gctttgatct tGCCTCTGTC TATTGCTTTC 2521 TGACTGTGTT CAGTCCTTTT ATGATGGGAG CCCTGATGAT GTGGAAGATT TTAATCCCCT 2581 TTGTTCTTGT TATGTGTGCT TTTGAAGCAG TTCAGTTGAC TACTCAGTTA TCGTCAAAAA 2641 GCCTTTTTCT CATTGTTCTC GTCATATCAG ACATTATGGC TTTGCATTTT TTCTTCTTGG 2701 TCAAGGATTA TGGCAGCTGG CTTGATATTG GGACAAGCAT CAGCCACTAT GTGATTGTCA 2761 TGTCCATGAC CATCTTTTTG GTGTTCCTCA ATGGCCTGGC CCAGCTGCTC ACAACGAAGA 2821 AACTCAGACT ATGTGGCAAA CCCAAAAGTC ACTTCATGTG CCCAACTTTC TTGTACAAAG 2881 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2941 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 3001 ACGACATGCC TACAGCTCCC ATTTCCCGAC GCGTTAAGTC gacaatcaac ctctggatta 3061 caaaatttgt gaaagatt