Construct: ORF TRCN0000479184
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011869.1_s317c1
- Derived from:
- ccsbBroadEn_07852
- DNA Barcode:
- CGAGTCAGGCCAAGCGTAAAACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSE1 (23199)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479184
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23199 | GSE1 | Gse1 coiled-coil protein | NM_001134473.3 | 99.9% | 99.9% | 2495T>C |
| 2 | human | 23199 | GSE1 | Gse1 coiled-coil protein | NM_001278184.3 | 97.2% | 97.2% | 1_93del;2588T>C |
| 3 | human | 23199 | GSE1 | Gse1 coiled-coil protein | XM_005255865.4 | 96.4% | 96.3% | 1_123del;2618T>C |
| 4 | human | 23199 | GSE1 | Gse1 coiled-coil protein | XM_011522966.3 | 91.6% | 91.5% | 1_303del;2798T>C |
| 5 | human | 23199 | GSE1 | Gse1 coiled-coil protein | XM_017023083.1 | 91.6% | 91.5% | 1_303del;2798T>C |
| 6 | human | 23199 | GSE1 | Gse1 coiled-coil protein | NM_014615.5 | 91.4% | 91.3% | 1_312del;2807T>C |
| 7 | human | 23199 | GSE1 | Gse1 coiled-coil protein | XM_005255864.4 | 90.6% | 90.6% | 1_342del;2837T>C |
| 8 | human | 23199 | GSE1 | Gse1 coiled-coil protein | XM_005255863.4 | 90.5% | 90.4% | 1_348del;2843T>C |
| 9 | human | 23199 | GSE1 | Gse1 coiled-coil protein | XM_011522965.3 | 88.8% | 88.7% | 1_420del;2915T>C |
| 10 | human | 23199 | GSE1 | Gse1 coiled-coil protein | XM_005255861.5 | 60% | 60% | 1_2217del;4712T>C |
| 11 | human | 23199 | GSE1 | Gse1 coiled-coil protein | XM_005255859.5 | 57.8% | 57.7% | 1_2436del;4931T>C |
| 12 | human | 23199 | GSE1 | Gse1 coiled-coil protein | XM_005255860.3 | 55.8% | 55.7% | 1_2436del;4767_4768ins114;4817T>C |
| 13 | mouse | 382034 | Gse1 | genetic suppressor element ... | XM_006531149.3 | 82.2% | 86.8% | (many diffs) |
| 14 | mouse | 382034 | Gse1 | genetic suppressor element ... | XM_006531148.3 | 81.5% | 86% | (many diffs) |
| 15 | mouse | 382034 | Gse1 | genetic suppressor element ... | NM_001145897.1 | 77.5% | 81.8% | (many diffs) |
| 16 | mouse | 382034 | Gse1 | genetic suppressor element ... | NM_001145896.1 | 77.3% | 81.6% | (many diffs) |
| 17 | mouse | 382034 | Gse1 | genetic suppressor element ... | NM_198671.2 | 76.7% | 80.9% | (many diffs) |
| 18 | mouse | 382034 | Gse1 | genetic suppressor element ... | XM_006531147.3 | 62.3% | 65.8% | (many diffs) |
| 19 | mouse | 382034 | Gse1 | genetic suppressor element ... | XM_006531145.3 | 59.5% | 62.8% | (many diffs) |
| 20 | mouse | 382034 | Gse1 | genetic suppressor element ... | XM_006531146.3 | 57.4% | 60.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3405
- ORF length:
- 3339
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ccctatcatc gtcccccctg ggggccacag cgtgcccagc accccccccg 121 tggtgaccat cgctccaacc aaaaccgtga atggtgtctg gaggagtgag agccggcagg 181 atgccggctc caggagcagc agtggaggtc gggaacgcct cattgtggag cccccgctcc 241 ctcaggagaa ggcaggggga ccagccatcc cctcgcacct gctcagcacc ccctacccct 301 tcggcctctc ccccagctca gttgtgcagg attcccgctt cccgccactc aacctccagc 361 ggcccgtgca ccacgtggtg ccccccagta ccgtgaccga ggactacctg agaagcttcc 421 ggccctacca caccaccgac gacctccgca tgtcctcact gcctcccctc ggcctggacc 481 cggccactgc tgcagcctac taccacccca gctacctggc cccacacccc ttcccccacc 541 cggccttcag gatggacgac tcctactgcc tgtctgccct gaggtccccg ttctacccca 601 tccccacccc cggctccctg cccccactgc acccatcagc gatgcacctg cacctctctg 661 gggtccgcta ccctcccgag ctctcccact catccctggc agcgctgcac tcggagcgca 721 tgtctggcct cagcgcggag aggctgcaga tggacgagga gctaaggcgg gagagggagc 781 gcgagcgcga gcgcgagcgt gagcgtgagg ctgaccgcga gcgggagaag gaacgtgagc 841 gcgaacgcga gaaggagcgc gagcaagaga aggagcgtga gcgtgagaag gagcgcgagc 901 gcgagctgga gcgccagcgg gagcagcggg cccgggagaa ggagctgctg gccgccaagg 961 ccctggagcc cagcttcctg cccgtggccg agctgcatgg gctgcgtggc catgccactg 1021 aggagcgggg caagccctcg gagcagctga ccccaacccg agcagagaag ctgaaggatg 1081 ccggcctgca ggcgcccaag cccgtccaac accccttgca tccggtgccc accccacacc 1141 acacggtgcc cagcctcatc tccaaccatg gcatcttctc tctgcctagc agcagtgctg 1201 ccacagccct gctgatccag cgcaccaatg aggaggagaa gtggctggcg cggcagcggc 1261 ggctgcggca ggagaaggag gaccggcagt ctcaggtgtc cgagttccgg cagcaggtgc 1321 tggagcagca cctggatatg ggccggcccc cggtgccggc ggaggcagag cacaggccgg 1381 agagcaccac caggccagga ccaaaccgtc acgagccagg tggccgtgac cctccgcagc 1441 actttggggg gccaccacct ctgatttcgc ccaagcccca gctccatgct gcacccacgg 1501 ccctctggaa ccccgtgtcc ctgatggaca acaccttgga gacgcggcgg gccgaaagcc 1561 actctctgca cagccacccg gctgcatttg agcccagccg ccaggcagcc gtgccgctgg 1621 tgaaggtgga gcgggtcttc tgcccggaga aagcagagga ggggccacgg aagcgtgagc 1681 ctgcccctct ggacaagtac cagccacctc cgccgccacc acgagaggga gggagcctgg 1741 agcaccagcc cttcctgccc gggcccgggc ccttcctggc tgagctcgag aagtccaccc 1801 agaccatcct gggccagcag cgggcctccc tcccacaggc ggccaccttc ggggagctca 1861 gcggacccct gaagcctggc tcgccctacc ggcccccagt gccacgggcc cccgaccctg 1921 cctacatcta tgatgagttc ctgcagcagc gccggaggct ggtcagcaag ctggacctgg 1981 aggagcgcag gcggcgggag gcccaggaga aagggtacta ctacgacctc gatgactctt 2041 acgacgagag cgatgaggag gaggtcaggg cccacctccg ttgcgtggcc gagcagccgc 2101 ccctcaaact ggacacgtcc tctgagaagc tagagttttt gcaacttttt ggcttgacca 2161 cccaacagca gaaggaggaa ttggtggccc agaagcggag gaagcggcgg aggatgctgc 2221 gagagagaag cccgtcgccc ccaacaattc agagcaagcg gcagacgcct tcaccgagac 2281 tggcgctgtc tacccgctac agccctgatg agatgaacaa cagtcccaac ttcgaagaaa 2341 agaagaagtt cctgaccatc ttcaacctga cccacatcag cgctgagaag aggaaagaca 2401 aagagagact tgttgaaatg ctccgtgcca tgaagcagaa ggcactgtca gcagcagtgg 2461 ccgactcctt gacaaactct ccgagggaca gtcctgccgt ctccctgagt gaaccagcca 2521 cgcagcaagc ctctctggat gtggagaagc cggttggtgc tgctgcttcc ttgtctgaca 2581 tcccaaaggc cgcggagcct gggaagctgg aacaggtccg gccccaggag ctgtcgagag 2641 tccaggagct agctcctgcc agcggggaga aggccaggct gagcgaggcc cctggaggca 2701 aaaagagtct gagcatgctt cactatatcc ggggcgctgc acccaaggac attcctgtgc 2761 cgctgtccca cagcaccaat gggaagagca agccgtggga gccctttgtg gcagaagagt 2821 ttgcacatca gttccacgag tcagtgctgc agtccaccca gaaggccctg cagaagcata 2881 aagggagcgt ggctgtgctg tctgcagagc agaaccacaa ggttgacacg tccgtccact 2941 acaacattcc tgagctgcag tcctccagcc gcgcccctcc accccagcac aatgggcagc 3001 aggagccccc cactgcaagg aagggCCCCC CAACCCAGGA GTTGGACCGG GACTCGGAGG 3061 AGGAGGAAGA GGAGGATGAT GAAGATGGAG AAGATGAGGA GGAAGTCCCC AAGCGCAAGT 3121 GGCAAGGGAT CGAGGCCGTT TTTGAAGCTT ACCAGGAACA CATAGAAGAG CAAAATCTGG 3181 AGCGGCAGGT GTTACAGACA CAATGTAGAC GACTGGAGGC CCGGCACTAC AGCCTCAGCC 3241 TGACGGCAGA GCAGCTCTCC CACAGCGTGG CGGAGTTGAG GAGCCAGAAA CAGAAGATGG 3301 TCTCAGAAAG GGAGCGGCTC CAGGCAGAAC TGGACCACTT ACGAAAGTGC CTTGCCTTGC 3361 CTGCAATGCA CTGGCCTAGG GGCTACCTGA AGGGATATCC CAGGTGCCCA ACTTTCTTGT 3421 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3481 AGTAATGAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC 3541 GAGTCAGGCC AAGCGTAAAA CTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa 3601 tttgtgaaag att