Construct: ORF TRCN0000479679
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009966.1_s317c1
- Derived from:
- ccsbBroadEn_01732
- DNA Barcode:
- GCATCGCTGTTGTTTGACTCTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBA52 (7311)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479679
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001033930.3 | 100% | 100% | |
| 2 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321017.1 | 100% | 100% | |
| 3 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321018.2 | 100% | 100% | |
| 4 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321019.2 | 100% | 100% | |
| 5 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321020.2 | 100% | 100% | |
| 6 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_003333.5 | 100% | 100% | |
| 7 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321021.1 | 94.8% | 94.8% | 1_21del |
| 8 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | XM_005260052.2 | 82% | 82% | 294_377del |
| 9 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | XM_005260053.3 | 82% | 82% | 294_377del |
| 10 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | XM_005260054.2 | 82% | 82% | 294_377del |
| 11 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | XM_006722871.2 | 82% | 82% | 294_377del |
| 12 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321022.2 | 77.3% | 77.3% | 102_103ins87 |
| 13 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | XM_017027198.1 | 63% | 63% | 1_141del;435_518del |
| 14 | human | 7314 | UBB | ubiquitin B | NM_018955.4 | 40.8% | 27.1% | (many diffs) |
| 15 | human | 7316 | UBC | ubiquitin C | NM_021009.7 | 15.1% | 10.3% | (many diffs) |
| 16 | mouse | 22186 | Uba52 | ubiquitin A-52 residue ribo... | NM_001348227.1 | 90.3% | 100% | (many diffs) |
| 17 | mouse | 22186 | Uba52 | ubiquitin A-52 residue ribo... | NM_001348228.1 | 90.3% | 100% | (many diffs) |
| 18 | mouse | 22186 | Uba52 | ubiquitin A-52 residue ribo... | NM_001348229.1 | 90.3% | 100% | (many diffs) |
| 19 | mouse | 22186 | Uba52 | ubiquitin A-52 residue ribo... | NM_019883.4 | 90.3% | 100% | (many diffs) |
| 20 | mouse | 665964 | Gm7866 | predicted gene 7866 | XR_870905.2 | 59.4% | (many diffs) | |
| 21 | mouse | 629750 | Gm11517 | predicted gene 11517; ubiqu... | NR_033523.1 | 50.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 453
- ORF length:
- 384
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagatcttt gtgaagaccc tcactggcaa aaccaTCACC CTTGAGGTCG 121 AGCCCAGTGA CACCATTGAG AATGTCAAAG CCAAAATTCA AGACAAGGAG GGTATCCCAC 181 CTGACCAGCA GCGTCTGATA TTTGCCGGCA AACAGCTGGA GGATGGCCGC ACTCTCTCAG 241 ACTACAACAT CCAGAAAGAG TCCACCCTGC ACCTGGTGTT GCGCCTGCGA GGTGGCATTA 301 TTGAGCCTTC TCTCCGCCAG CTTGCCCAGA AATACAACTG CGACAAGATG ATCTGCCGCA 361 AGTGCTATGC TCGCCTTCAC CCTCGTGCTG TCAACTGCCG CAAGAAGAAG TGTGGTCACA 421 CCAACAACCT GCGTCCCAAG AAGAAGGTCA AATTGCCAAC TTTCTTGTAC AAAGTGGTTG 481 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 541 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGC 601 ATCGCTGTTG TTTGACTCTT AGACGCGTTA AGTCgacaat caacctctgg attacaaaat 661 ttgtgaaaga tt