Transcript: Human NM_001321019.2

Homo sapiens ubiquitin A-52 residue ribosomal protein fusion product 1 (UBA52), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
UBA52 (7311)
Length:
3084
CDS:
298..684

Additional Resources:

NCBI RefSeq record:
NM_001321019.2
NBCI Gene record:
UBA52 (7311)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001321019.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000003999 CCGCAAGAAGAAGTGTGGTCA pLKO.1 627 CDS 100% 2.640 3.696 N UBA52 n/a
2 TRCN0000272707 CCGCAAGAAGAAGTGTGGTCA pLKO_005 627 CDS 100% 2.640 3.696 N UBA52 n/a
3 TRCN0000003998 GCGTCTGATATTTGCCGGCAA pLKO.1 420 CDS 100% 2.160 3.024 N UBA52 n/a
4 TRCN0000003996 AGCGTCTGATATTTGCCGGCA pLKO.1 419 CDS 100% 0.540 0.432 N UBA52 n/a
5 TRCN0000271957 CAAGATGATCTGCCGCAAGTG pLKO_005 573 CDS 100% 4.050 2.835 N Gm11808 n/a
6 TRCN0000003997 GCGTCCCAAGAAGAAGGTCAA pLKO.1 660 CDS 100% 4.050 2.835 N UBA52 n/a
7 TRCN0000272708 GCGTCCCAAGAAGAAGGTCAA pLKO_005 660 CDS 100% 4.050 2.835 N UBA52 n/a
8 TRCN0000010841 CACCAACAACCTGCGTCCCAA pLKO.1 648 CDS 100% 0.880 0.616 N UBA52 n/a
9 TRCN0000272656 CACCAACAACCTGCGTCCCAA pLKO_005 648 CDS 100% 0.880 0.616 N UBA52 n/a
10 TRCN0000272655 AGAACGTTGATTCTCAAATTT pLKO_005 1109 3UTR 100% 15.000 9.000 N UBA52 n/a
11 TRCN0000098659 TCAGACTACAACATCCAGAAA pLKO.1 466 CDS 100% 4.950 2.970 N Ubc n/a
12 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 2601 3UTR 100% 5.625 2.813 Y KLHL30 n/a
13 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 2601 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001321019.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01732 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01732 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000479679 GCATCGCTGTTGTTTGACTCTTAG pLX_317 87.9% 100% 100% V5 n/a
4 TRCN0000468005 TCGGGCCTGTCTTATCTTATATCC pLX_317 100% 50.2% 59.3% V5 (many diffs) n/a
5 ccsbBroadEn_14872 pDONR223 58.4% 40.7% 27.1% None (many diffs) n/a
6 ccsbBroad304_14872 pLX_304 0% 40.7% 27.1% V5 (many diffs) n/a
7 ccsbBroadEn_14873 pDONR223 70.1% 42.2% 27.1% None (many diffs) n/a
8 ccsbBroad304_14873 pLX_304 0% 42.2% 27.1% V5 (many diffs) n/a
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