Transcript: Human NM_001321022.2

Homo sapiens ubiquitin A-52 residue ribosomal protein fusion product 1 (UBA52), transcript variant 8, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
UBA52 (7311)
Length:
2761
CDS:
62..361

Additional Resources:

NCBI RefSeq record:
NM_001321022.2
NBCI Gene record:
UBA52 (7311)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001321022.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000003999 CCGCAAGAAGAAGTGTGGTCA pLKO.1 304 CDS 100% 2.640 3.696 N UBA52 n/a
2 TRCN0000272707 CCGCAAGAAGAAGTGTGGTCA pLKO_005 304 CDS 100% 2.640 3.696 N UBA52 n/a
3 TRCN0000271957 CAAGATGATCTGCCGCAAGTG pLKO_005 250 CDS 100% 4.050 2.835 N Gm11808 n/a
4 TRCN0000003997 GCGTCCCAAGAAGAAGGTCAA pLKO.1 337 CDS 100% 4.050 2.835 N UBA52 n/a
5 TRCN0000272708 GCGTCCCAAGAAGAAGGTCAA pLKO_005 337 CDS 100% 4.050 2.835 N UBA52 n/a
6 TRCN0000010841 CACCAACAACCTGCGTCCCAA pLKO.1 325 CDS 100% 0.880 0.616 N UBA52 n/a
7 TRCN0000272656 CACCAACAACCTGCGTCCCAA pLKO_005 325 CDS 100% 0.880 0.616 N UBA52 n/a
8 TRCN0000272655 AGAACGTTGATTCTCAAATTT pLKO_005 786 3UTR 100% 15.000 9.000 N UBA52 n/a
9 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 2278 3UTR 100% 5.625 2.813 Y KLHL30 n/a
10 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 2278 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001321022.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01732 pDONR223 100% 77.3% 77.3% None 102_103ins87 n/a
2 ccsbBroad304_01732 pLX_304 0% 77.3% 77.3% V5 102_103ins87 n/a
3 TRCN0000479679 GCATCGCTGTTGTTTGACTCTTAG pLX_317 87.9% 77.3% 77.3% V5 102_103ins87 n/a
4 TRCN0000468005 TCGGGCCTGTCTTATCTTATATCC pLX_317 100% 54.1% 36.7% V5 (many diffs) n/a
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