Transcript: Mouse NM_001348227.1

Mus musculus ubiquitin A-52 residue ribosomal protein fusion product 1 (Uba52), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Uba52 (22186)
Length:
565
CDS:
89..475

Additional Resources:

NCBI RefSeq record:
NM_001348227.1
NBCI Gene record:
Uba52 (22186)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001348227.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000347704 GACCAGCAGAGGCTGATATTC pLKO_005 203 CDS 100% 13.200 6.600 Y Gm11808 n/a
2 TRCN0000272013 CCAGTGACACCATCGAGAATG pLKO_005 144 CDS 100% 10.800 5.400 Y Gm11808 n/a
3 TRCN0000271956 CCGACTACAACATCCAGAAAG pLKO_005 258 CDS 100% 10.800 5.400 Y Gm11808 n/a
4 TRCN0000087138 CCAGAAGTACAACTGTGACAA pLKO.1 346 CDS 100% 4.950 2.475 Y Uba52 n/a
5 TRCN0000087139 CGAGAATGTCAAGGCCAAGAT pLKO.1 157 CDS 100% 4.950 2.475 Y Uba52 n/a
6 TRCN0000087142 TCCGACTACAACATCCAGAAA pLKO.1 257 CDS 100% 4.950 2.475 Y Uba52 n/a
7 TRCN0000347701 TTCGTCAGCTTGCCCAGAAGT pLKO_005 333 CDS 100% 4.950 2.475 Y Gm11808 n/a
8 TRCN0000271957 CAAGATGATCTGCCGCAAGTG pLKO_005 364 CDS 100% 4.050 2.025 Y Gm11808 n/a
9 TRCN0000087140 GCCAAGATCCAAGACAAGGAA pLKO.1 170 CDS 100% 3.000 1.500 Y Uba52 n/a
10 TRCN0000086705 GATCCAAGACAAGGAAGGCAT pLKO.1 175 CDS 100% 2.640 1.320 Y LOC333475 n/a
11 TRCN0000086860 GCCCAGTGACACCATCGAGAA pLKO.1 142 CDS 100% 1.350 0.675 Y Gm1821 n/a
12 TRCN0000087141 CCGCAAGTGCTACGCACGCCT pLKO.1 376 CDS 100% 0.000 0.000 Y Uba52 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001348227.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01732 pDONR223 100% 90.3% 100% None (many diffs) n/a
2 ccsbBroad304_01732 pLX_304 0% 90.3% 100% V5 (many diffs) n/a
3 TRCN0000479679 GCATCGCTGTTGTTTGACTCTTAG pLX_317 87.9% 90.3% 100% V5 (many diffs) n/a
4 ccsbBroadEn_14873 pDONR223 70.1% 46.3% 27.1% None (many diffs) n/a
5 ccsbBroad304_14873 pLX_304 0% 46.3% 27.1% V5 (many diffs) n/a
6 TRCN0000468005 TCGGGCCTGTCTTATCTTATATCC pLX_317 100% 52% 59.3% V5 (many diffs) n/a
7 ccsbBroadEn_14872 pDONR223 58.4% 41.9% 27.1% None (many diffs) n/a
8 ccsbBroad304_14872 pLX_304 0% 41.9% 27.1% V5 (many diffs) n/a
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