Construct: ORF TRCN0000479791

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002741.1_s317c1
Derived from:
ccsbBroadEn_00076
DNA Barcode:
AAGTGCTCTTAAAATGCCCAGAAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
APBB1 (322)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479791
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 322 APBB1 amyloid beta precursor prot... NM_145689.3 100% 100%
2 human 322 APBB1 amyloid beta precursor prot... NM_001164.5 99.7% 99.7% 1381_1386delAGAGAG
3 human 322 APBB1 amyloid beta precursor prot... NR_047512.2 87.4% (many diffs)
4 human 322 APBB1 amyloid beta precursor prot... NM_001257323.2 68% 66.9% (many diffs)
5 human 322 APBB1 amyloid beta precursor prot... NM_001257319.2 67.8% 66.7% (many diffs)
6 human 322 APBB1 amyloid beta precursor prot... NM_001257325.2 66% 65.9% (many diffs)
7 human 322 APBB1 amyloid beta precursor prot... NM_001257320.2 63.2% 63.2% 0_1ins777;604_609delAGAGAG
8 human 322 APBB1 amyloid beta precursor prot... NM_001257321.2 63.2% 63.2% 0_1ins777;604_609delAGAGAG
9 human 322 APBB1 amyloid beta precursor prot... NM_001257326.2 63.2% 63.2% 0_1ins777;604_609delAGAGAG
10 mouse 11785 Apbb1 amyloid beta (A4) precursor... NM_001253886.1 90.4% 94.6% (many diffs)
11 mouse 11785 Apbb1 amyloid beta (A4) precursor... NM_009685.3 90.4% 94.6% (many diffs)
12 mouse 11785 Apbb1 amyloid beta (A4) precursor... XM_017321946.1 90.4% 94.6% (many diffs)
13 mouse 11785 Apbb1 amyloid beta (A4) precursor... NM_001253885.1 90.1% 94.3% (many diffs)
14 mouse 11785 Apbb1 amyloid beta (A4) precursor... XM_006507230.2 90.1% 94.3% (many diffs)
15 mouse 11785 Apbb1 amyloid beta (A4) precursor... XM_006507231.1 90.1% 94.3% (many diffs)
16 mouse 11785 Apbb1 amyloid beta (A4) precursor... XM_006507232.3 64.1% 64.5% (many diffs)
17 mouse 11785 Apbb1 amyloid beta (A4) precursor... NM_001310600.1 61.7% 64.2% (many diffs)
18 mouse 11785 Apbb1 amyloid beta (A4) precursor... XM_017321947.1 61.6% 64% (many diffs)
19 mouse 11785 Apbb1 amyloid beta (A4) precursor... NM_001253887.1 57.9% 60.7% (many diffs)
20 mouse 11785 Apbb1 amyloid beta (A4) precursor... XM_006507234.3 57.9% 60.7% (many diffs)
21 mouse 11785 Apbb1 amyloid beta (A4) precursor... XM_006507235.3 57.9% 60.7% (many diffs)
22 mouse 11785 Apbb1 amyloid beta (A4) precursor... XM_006507236.3 57.9% 60.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2190
ORF length:
2124
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc tgttccatca tcactgagcc agtcggccat taatgccaac agccacggag
121gccccgcact gagcctaccc ctgcctctgc acgctgccca caaccagctg ctcaacgcca
181agctgcaggc cacagctgtg ggacccaagg acctgcgcag cgccatgggg gagggtggtg
241ggcctgagcc aggccctgcc aatgccaagt ggctaaaaga gggccagaac cagctccggc
301gggccgccac ggcccaccgt gaccagaatc gcaatgtgac cttgaccttg gcggaggagg
361ccagccagga gcctgagatg gcacccttgg gccccaaagg cctgatacac ctgtactctg
421agctggagct ctcagctcac aacgcagcca accgaggcct acgaggacct ggcctgatca
481tcagcactca agagcagggg ccagatgagg gagaggagaa ggcggccggg gaggccgagg
541aggaggagga ggatgatgat gatgaagagg aggaggagga cttatcttct cccccagggc
601tgcctgagcc cctggagagt gtggaggccc ctcccaggcc ccaagccctt acagatggcc
661cccgggaaca cagcaagagt gccagcctcc tgtttggcat gcggaacagt gcagccagtg
721atgaggactc aagctgggct accttatccc agggcagccc ctcctatggc tccccagagg
781acacagattc cttctggaac cccaacgcct tcgagacgga ttccgacctg ccggctggat
841ggatgagggt ccaggacacc tcagggacct attactggca catcccaaca gggaccaccc
901agtgggaacc ccccggccgg gcctccccct cacaggggag cagcccccaa gaggagtccc
961agctcacctg gacaggtttt gctcatggag aaggctttga ggatggagaa ttttggaagg
1021atgaacccag tgatgaggcc ccaatggagc tgggactgaa ggaacctgag gaggggacgt
1081tgaccttccc agctcagagc ctcagcccag agccgttgcc ccaagaggag gagaagcttc
1141ccccacggaa taccaaccca gggatcaagt gtttcgccgt gcgctcccta ggctgggtag
1201agatgaccga ggaggagctg gcccctggac gcagcagtgt ggcagtcaac aattgcatcc
1261gtcagctctc ttaccacaaa aacaacctgc atgaccccat gtctgggggc tggggggaag
1321gaaaggatct gctactgcag ctggaggatg agacactaaa gctagtggag ccacagagcc
1381aggcactgct gcacgcccaa cccatcatca gcatccgcgt gtggggcgtc gggcgggaca
1441gtggaaggga ctttgcctac gtagctcgtg ataagctgac ccagatgctc aagtgccacg
1501tgtttcgctg tgaggcacct gccaagaaca tcgccaccag cctgcatgag atctgctcta
1561agatcatggc cgaacggcgt aatgcccgct gcttggtaaa tggactctcc ctggaccact
1621ctaaacttgt ggatgtccct ttccaagtgg aattcccagc gcctaagaat gagttggtcc
1681agaagttcca agtctattac ctggggaatg tacctgttgc taaacctgtt ggggtagatg
1741tgattaatgg ggccctcgag tcagtcctgt ccTCCAGCAG CCGTGAACAA TGGACCCCAA
1801GTCATGTCAG TGTGGCCCCT GCTACCCTCA CCATCTTGCA CCAGCAGACA GAGGCAGTGC
1861TGGGAGAGTG TCGGGTGCGT TTCCTCTCCT TCCTGGCCGT GGGCAGAGAT GTCCACACGT
1921TTGCATTCAT CATGGCTGCC GGCCCAGCCT CCTTCTGCTG CCACATGTTC TGGTGCGAGC
1981CCAATGCTGC CAGCCTCTCA GAGGCTGTGC AGGCTGCGTG CATGCTTCGC TACCAGAAGT
2041GTCTGGATGC CCGTTCCCAG GCCTCCACCT CCTGCCTCCC AGCACCCCCT GCTGAGTCTG
2101TGGCACGGCG TGTAGGGTGG ACTGTCCGCA GGGGTGTTCA GTCGCTGTGG GGCTCCCTGA
2161AGCCCAAACG GCTGGGGGCC CATACCCCAT GCCCAACTTT CTTGTACAAA GTGGTTGATA
2221TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
2281CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAAGTG
2341CTCTTAAAAT GCCCAGAAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
2401tgaaagatt

Download FASTA (ORF) (Full)