Construct: ORF TRCN0000479800
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011972.1_s317c1
- Derived from:
- ccsbBroadEn_01551
- DNA Barcode:
- ACTTAGTCTTTACCCATGGCTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC20A1 (6574)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479800
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6574 | SLC20A1 | solute carrier family 20 me... | NM_005415.5 | 99.9% | 100% | 303G>A |
| 2 | human | 6574 | SLC20A1 | solute carrier family 20 me... | XM_024453052.1 | 68.6% | 68.6% | 0_1ins639 |
| 3 | human | 6574 | SLC20A1 | solute carrier family 20 me... | XM_017004770.2 | 62.7% | 62.7% | 0_1ins759 |
| 4 | human | 6574 | SLC20A1 | solute carrier family 20 me... | XM_017004771.2 | 62.7% | 62.7% | 0_1ins759 |
| 5 | mouse | 20515 | Slc20a1 | solute carrier family 20, m... | NM_015747.2 | 88.1% | 90.3% | (many diffs) |
| 6 | mouse | 20515 | Slc20a1 | solute carrier family 20, m... | XM_011239399.2 | 55.2% | 57.1% | (many diffs) |
| 7 | mouse | 20515 | Slc20a1 | solute carrier family 20, m... | XM_017316753.1 | 52.8% | 54.7% | (many diffs) |
| 8 | mouse | 20515 | Slc20a1 | solute carrier family 20, m... | NM_001159593.1 | 30.1% | 29.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2103
- ORF length:
- 2037
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aacgctgatt accagtacta cagctgctac cgccgcttct ggtcctttgg 121 tggactacct atggatgctc atcctgggct tcattattgc atttgtcttg gcattctccg 181 tgggagccaa tgatgtagca aattcttttg gtacagctgt gggctcaggt gtagtgaccc 241 tgaagcaagc ctgcatccta gctagcatct ttgaaacagt gggctctgtc ttactggggg 301 ccaaagtgag cgaaaccatc cggaagggct tgattgacgt ggagatgtac aactcgactc 361 aagggctact gatggccggc tcagtcagtg ctatgtttgg ttctgctgtg tggcaactcg 421 tggcttcgtt tttgaagctc cctatttctg gaacccattg tattgttggt gcaactattg 481 gtttctccct cgtggcaaag gggcaggagg gtgtcaagtg gtctgaactg ataaaaattg 541 tgatgtcttg gttcgtgtcc ccactgcttt ctggaattat gtctggaatt ttattcttcc 601 tggttcgtgc attcatcctc cataaggcag atccagttcc taatggtttg cgagctttgc 661 cagttttcta tgcctgcaca gttggaataa acctcttttc catcatgtat actggagcac 721 cgttgctggg ctttgacaaa cttcctctgt ggggtaccat cctcatctcg gtgggatgtg 781 cagttttctg tgcccttatc gtctggttct ttgtatgtcc caggatgaag agaaaaattg 841 aacgagaaat aaagtgtagt ccttctgaaa gccccttaat ggaaaaaaag aatagcttga 901 aagaagacca tgaagaaaca aagttgtctg ttggtgatat tgaaaacaag catcctgttt 961 ctgaggtagg gcctgccact gtgcccctcc aggctgtggt ggaggagaga acagtctcat 1021 tcaaacttgg agatttggag gaagctccag agagagagag gcttcccagc gtggacttga 1081 aagaggaaac cagcatagat agcaccgtga atggtgcagt gcagttgcct aatgggaacc 1141 ttgtccagtt cagtcaagcc gtcagcaacc aaataaactc cagtggccac taccagtatc 1201 acaccgtgca taaggattcc ggcctgtaca aagagctact ccataaatta catcttgcca 1261 aggtgggaga ttgcatggga gactccggtg acaaaccctt aaggcgcaat aatagctata 1321 cttcctatac catggcaata tgtggcatgc ctctggattc attccgtgcc aaagaaggtg 1381 aacagaaggg cgaagaaatg gagaagctga catggcctaa tgcagactcc aagaagcgaa 1441 ttcgaatgga cagttacacc agttactgca atgctgtgtc tgaccttcac tcagcatctg 1501 agatagacat gagtgtcaag gcagagatgg gtctaggtga cagaaaagga agtaatggct 1561 ctctagaaga atggtatgac caggataagc ctgaagtctc tctcctcttc cagttcctgc 1621 agatccttac agcctgcttt gggtcattcg cccatggtgg caatgacgta agcaatgcca 1681 ttgggcctct ggttgcttta tatttggttt atgacacagg agatgtttct tcaaaagtgg 1741 caacaccaat atggcttcta ctctatggtg gtgttggtat ctgtgttggt ctgtgggttt 1801 ggggaagaag agttatccag accatgggga aggatctgac accgatcaca ccctctagtg 1861 gcttcagtat tgaactggca tctgccctca ctgtggtgat tgcatcaaat attggccttc 1921 ccatCAGTAC AACACATTGT AAAGTGGGCT CTGTTGTGTC TGTTGGCTGG CTCCGGTCCA 1981 AGAAGGCTGT TGACTGGCGT CTCTTTCGTA ACATTTTTAT GGCCTGGTTT GTCACAGTCC 2041 CCATTTCTGG AGTTATCAGT GCTGCCATCA TGGCAATCTT CAGATATGTC ATCCTCAGAA 2101 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2161 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2221 GGCTTTATAT ATCTTGTGGA AAGGACGAAC TTAGTCTTTA CCCATGGCTT CAACGCGTTA 2281 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt