Construct: ORF TRCN0000479800

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011972.1_s317c1
Derived from:
ccsbBroadEn_01551
DNA Barcode:
ACTTAGTCTTTACCCATGGCTTCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC20A1 (6574)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479800
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6574 SLC20A1 solute carrier family 20 me... NM_005415.5 99.9% 100% 303G>A
2 human 6574 SLC20A1 solute carrier family 20 me... XM_024453052.1 68.6% 68.6% 0_1ins639
3 human 6574 SLC20A1 solute carrier family 20 me... XM_017004770.2 62.7% 62.7% 0_1ins759
4 human 6574 SLC20A1 solute carrier family 20 me... XM_017004771.2 62.7% 62.7% 0_1ins759
5 mouse 20515 Slc20a1 solute carrier family 20, m... NM_015747.2 88.1% 90.3% (many diffs)
6 mouse 20515 Slc20a1 solute carrier family 20, m... XM_011239399.2 55.2% 57.1% (many diffs)
7 mouse 20515 Slc20a1 solute carrier family 20, m... XM_017316753.1 52.8% 54.7% (many diffs)
8 mouse 20515 Slc20a1 solute carrier family 20, m... NM_001159593.1 30.1% 29.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2103
ORF length:
2037
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc aacgctgatt accagtacta cagctgctac cgccgcttct ggtcctttgg
121tggactacct atggatgctc atcctgggct tcattattgc atttgtcttg gcattctccg
181tgggagccaa tgatgtagca aattcttttg gtacagctgt gggctcaggt gtagtgaccc
241tgaagcaagc ctgcatccta gctagcatct ttgaaacagt gggctctgtc ttactggggg
301ccaaagtgag cgaaaccatc cggaagggct tgattgacgt ggagatgtac aactcgactc
361aagggctact gatggccggc tcagtcagtg ctatgtttgg ttctgctgtg tggcaactcg
421tggcttcgtt tttgaagctc cctatttctg gaacccattg tattgttggt gcaactattg
481gtttctccct cgtggcaaag gggcaggagg gtgtcaagtg gtctgaactg ataaaaattg
541tgatgtcttg gttcgtgtcc ccactgcttt ctggaattat gtctggaatt ttattcttcc
601tggttcgtgc attcatcctc cataaggcag atccagttcc taatggtttg cgagctttgc
661cagttttcta tgcctgcaca gttggaataa acctcttttc catcatgtat actggagcac
721cgttgctggg ctttgacaaa cttcctctgt ggggtaccat cctcatctcg gtgggatgtg
781cagttttctg tgcccttatc gtctggttct ttgtatgtcc caggatgaag agaaaaattg
841aacgagaaat aaagtgtagt ccttctgaaa gccccttaat ggaaaaaaag aatagcttga
901aagaagacca tgaagaaaca aagttgtctg ttggtgatat tgaaaacaag catcctgttt
961ctgaggtagg gcctgccact gtgcccctcc aggctgtggt ggaggagaga acagtctcat
1021tcaaacttgg agatttggag gaagctccag agagagagag gcttcccagc gtggacttga
1081aagaggaaac cagcatagat agcaccgtga atggtgcagt gcagttgcct aatgggaacc
1141ttgtccagtt cagtcaagcc gtcagcaacc aaataaactc cagtggccac taccagtatc
1201acaccgtgca taaggattcc ggcctgtaca aagagctact ccataaatta catcttgcca
1261aggtgggaga ttgcatggga gactccggtg acaaaccctt aaggcgcaat aatagctata
1321cttcctatac catggcaata tgtggcatgc ctctggattc attccgtgcc aaagaaggtg
1381aacagaaggg cgaagaaatg gagaagctga catggcctaa tgcagactcc aagaagcgaa
1441ttcgaatgga cagttacacc agttactgca atgctgtgtc tgaccttcac tcagcatctg
1501agatagacat gagtgtcaag gcagagatgg gtctaggtga cagaaaagga agtaatggct
1561ctctagaaga atggtatgac caggataagc ctgaagtctc tctcctcttc cagttcctgc
1621agatccttac agcctgcttt gggtcattcg cccatggtgg caatgacgta agcaatgcca
1681ttgggcctct ggttgcttta tatttggttt atgacacagg agatgtttct tcaaaagtgg
1741caacaccaat atggcttcta ctctatggtg gtgttggtat ctgtgttggt ctgtgggttt
1801ggggaagaag agttatccag accatgggga aggatctgac accgatcaca ccctctagtg
1861gcttcagtat tgaactggca tctgccctca ctgtggtgat tgcatcaaat attggccttc
1921ccatCAGTAC AACACATTGT AAAGTGGGCT CTGTTGTGTC TGTTGGCTGG CTCCGGTCCA
1981AGAAGGCTGT TGACTGGCGT CTCTTTCGTA ACATTTTTAT GGCCTGGTTT GTCACAGTCC
2041CCATTTCTGG AGTTATCAGT GCTGCCATCA TGGCAATCTT CAGATATGTC ATCCTCAGAA
2101TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
2161TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
2221GGCTTTATAT ATCTTGTGGA AAGGACGAAC TTAGTCTTTA CCCATGGCTT CAACGCGTTA
2281AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)