Construct: ORF TRCN0000479938
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007981.1_s317c1
- Derived from:
- ccsbBroadEn_10490
- DNA Barcode:
- GTACCAGTTGGTCTCCCACTAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GK3P (2713)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479938
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2710 | GK | glycerol kinase | NM_001128127.2 | 97.8% | 98.3% | (many diffs) |
| 2 | human | 2710 | GK | glycerol kinase | NM_001205019.1 | 96.8% | 97.3% | (many diffs) |
| 3 | human | 2710 | GK | glycerol kinase | XM_006724483.2 | 93.1% | 93.6% | (many diffs) |
| 4 | human | 2710 | GK | glycerol kinase | NM_000167.5 | 92.5% | 93.3% | (many diffs) |
| 5 | human | 2710 | GK | glycerol kinase | XM_011545491.2 | 92.2% | 92.6% | (many diffs) |
| 6 | human | 2710 | GK | glycerol kinase | NM_203391.3 | 91.5% | 92.3% | (many diffs) |
| 7 | human | 2710 | GK | glycerol kinase | XM_006724484.2 | 88% | 88.8% | (many diffs) |
| 8 | human | 2712 | GK2 | glycerol kinase 2 | NM_033214.3 | 87.3% | 87.5% | (many diffs) |
| 9 | human | 2710 | GK | glycerol kinase | XM_011545492.1 | 87.1% | 87.9% | (many diffs) |
| 10 | human | 2713 | GK3P | glycerol kinase 3 pseudogene | NR_026575.1 | 74.2% | 1_378del;1831G>A;2038_2232del | |
| 11 | human | 2710 | GK | glycerol kinase | XM_005274488.4 | 61.4% | 61.6% | (many diffs) |
| 12 | human | 2710 | GK | glycerol kinase | XM_006724485.2 | 60.8% | 61% | (many diffs) |
| 13 | human | 2710 | GK | glycerol kinase | XM_006724486.3 | 60.8% | 61% | (many diffs) |
| 14 | human | 2710 | GK | glycerol kinase | XM_011545493.2 | 60.8% | 61% | (many diffs) |
| 15 | human | 2710 | GK | glycerol kinase | XM_011545494.2 | 60.8% | 61% | (many diffs) |
| 16 | human | 2710 | GK | glycerol kinase | XM_017029409.1 | 60.8% | 61% | (many diffs) |
| 17 | human | 2710 | GK | glycerol kinase | XM_017029411.1 | 56.1% | 56.6% | (many diffs) |
| 18 | human | 2710 | GK | glycerol kinase | XM_017029412.2 | 56.1% | 56.6% | (many diffs) |
| 19 | human | 2710 | GK | glycerol kinase | XM_017029410.1 | 55.5% | 55.9% | (many diffs) |
| 20 | mouse | 14933 | Gk | glycerol kinase | NM_212444.2 | 91% | 95.8% | (many diffs) |
| 21 | mouse | 14933 | Gk | glycerol kinase | NM_001294140.1 | 90% | 94.8% | (many diffs) |
| 22 | mouse | 14933 | Gk | glycerol kinase | XM_006527831.3 | 86.6% | 91.2% | (many diffs) |
| 23 | mouse | 14933 | Gk | glycerol kinase | NM_008194.3 | 85.8% | 90.7% | (many diffs) |
| 24 | mouse | 14933 | Gk | glycerol kinase | XM_006527829.3 | 85.7% | 90.2% | (many diffs) |
| 25 | mouse | 14933 | Gk | glycerol kinase | NM_001331046.1 | 84.9% | 89.8% | (many diffs) |
| 26 | mouse | 14933 | Gk | glycerol kinase | NM_001294138.1 | 81.6% | 86.4% | (many diffs) |
| 27 | mouse | 14933 | Gk | glycerol kinase | XM_006527833.3 | 80.8% | 85.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1725
- ORF length:
- 1659
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcctcaaag aaggcagttt tggggccatt ggtgggggca gtggaccaag 121 gcaccagttc cacgcgcttt ttggttttca attcaagaac agctgaacta cttagtcatc 181 atcaagtgga aataaaacaa gagttcccaa gagaaggatg ggtggaacag gaccctaagg 241 aaattctaca ttctgtctat gagtgtatag agaaaacatg tgagaaactt ggacagctca 301 atattggtat ttccaacata aaagctattg gtgtcagcaa ccagagggaa accaccgtag 361 tctgggacaa gataactgga gagcctctct acaatgctgt ggtgtggctt gatctaagaa 421 cacagtctac cgttgagagt cttagtaaaa gaattccagg aaataataac tttgtcaagt 481 ccaagacagg ccttccactt agcacttact tcagtgcagt gaaacttcgc tggctcctcg 541 acaatgtgag aaaagttcaa aaggccgttg aagaaaaacg agctcttttt gggactattg 601 attcatggct tatttggagt ttgacaggag gcgtcaatgg aggtgtccac tgtacagatg 661 taacaaatgc aagtaggact atgcttttca acattcattc tttggaatgg gataaacaac 721 tctgtgaatt ttttggaatt ccaatggaaa ttcttccaca tgttcggagt tcttctgaga 781 tctatggcct aatgaaagcg ggggccttgg aaggtgtgcc aatatctggg tgtttagggg 841 accagtctgc tgcactggtg ggacaaatgt gcttccagat tggacaagcc aaaaatacgt 901 atggaacagg atgtttctta ctatgtaata caggccataa gtgtgtattt tctgatcatg 961 gccttctcac cacagtggct tacaaacttg gcagagacaa accggtatat tacgctttgg 1021 aaggttctgt agctatagct ggtgctgtta ttcgctggct aagagacaat cttggaatta 1081 taaagacctc agaagaaatt gaaaaacttg ctaaagaagt aggtacttct tatggctgct 1141 acttcgtccc agcattttcg gggttatatg caccttattg ggagcccagc gcaagaggga 1201 taatctgtgg actcactcaa ttcacgaata aatgccatat tgcttttgct gcattagaag 1261 ctgtttgttt ccaaactcga gagattttgg atgccatgaa tcgagactgt ggaattccac 1321 tcagtcattT GCAGGTTGAT GGAGGAATGA CCAGCAACAA AATTCTTATG CAGCTACAAG 1381 CAGACATTCT GTATATTCCA GTAGTGAAGC CCTTGATGCC CGAAACCACT GCACTGGGTG 1441 CTGCCATGGC GGCAGGGGCT GCAGAAGGAG TCGACGTATG GAGTCTTGAA CCTGAGGATT 1501 TGTCCGCCGT CACGATGAAG CGGTTTGAAC CTCAGATTAA TGCTGAGGAA AGTGAAATTC 1561 GTTATTCTAC ATGGAAGAAA GCTGTGATGA AGTCAATGGG TTGGGTTACA ACTCAATCTC 1621 CAGAAGGTGG TGACCCTAGT GTCTTCTGTA GTCTGCCCTT GGGCTTTTTT ATAGTGAGTA 1681 GCATGGCAAT GTTAATCGGA GCAAGGTACA TCTCAGGTAT TCCATACCCA ACTTTCTTGT 1741 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1801 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1861 GAAAGGACGA GTACCAGTTG GTCTCCCACT AACCACGCGT TAAGTCgaca atcaacctct 1921 ggattacaaa atttgtgaaa gatt