Construct: ORF TRCN0000480014
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019059.4_s317c1
- Derived from:
- ccsbBroadEn_14546
- DNA Barcode:
- GTACGCGAAGCTCCCAGGAGAAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- AXL (558)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480014
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 558 | AXL | AXL receptor tyrosine kinase | NM_021913.5 | 100% | 100% | |
| 2 | human | 558 | AXL | AXL receptor tyrosine kinase | NM_001699.6 | 98.9% | 98.9% | 1284_1285ins27 |
| 3 | human | 558 | AXL | AXL receptor tyrosine kinase | NM_001278599.1 | 70% | 70% | 0_1ins804 |
| 4 | mouse | 26362 | Axl | AXL receptor tyrosine kinase | NM_009465.4 | 84.9% | 87.5% | (many diffs) |
| 5 | mouse | 26362 | Axl | AXL receptor tyrosine kinase | XM_006539989.3 | 84.9% | 87.5% | (many diffs) |
| 6 | mouse | 26362 | Axl | AXL receptor tyrosine kinase | NM_001190974.1 | 84.1% | 86.9% | (many diffs) |
| 7 | mouse | 26362 | Axl | AXL receptor tyrosine kinase | XM_006539991.3 | 84.1% | 86.9% | (many diffs) |
| 8 | mouse | 26362 | Axl | AXL receptor tyrosine kinase | NM_001190975.1 | 75.8% | 78.6% | (many diffs) |
| 9 | mouse | 26362 | Axl | AXL receptor tyrosine kinase | XM_006539992.2 | 75.8% | 78.6% | (many diffs) |
| 10 | mouse | 26362 | Axl | AXL receptor tyrosine kinase | XM_006539993.2 | 75% | 77.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2751
- ORF length:
- 2682
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgtggcgg tgccccagga tgggcagggt cccgctggcc tggtgcttgg 121 cgctgtgcgg ctgggcgtgc atggccccca ggggcacgca ggctgaagaa agtcccttcg 181 tgggcaaccc agggaatatc acaggtgccc ggggactcac gggcaccctt cggtgtcagc 241 tccaggttca gggagagccc cccgaggtac attggcttcg ggatggacag atcctggagc 301 tcgcggacag cacccagacc caggtgcccc tgggtgagga tgaacaggat gactggatag 361 tggtcagcca gctcagaatc acctccctgc agctttccga cacgggacag taccagtgtt 421 tggtgtttct gggacatcag accttcgtgt cccagcctgg ctatgttggg ctggagggct 481 tgccttactt cctggaggag cccgaagaca ggactgtggc cgccaacacc cccttcaacc 541 tgagctgcca agctcaggga cccccagagc ccgtggacct actctggctc caggatgctg 601 tccccctggc cacggctcca ggtcacggcc cccagcgcag cctgcatgtt ccagggctga 661 acaagacatc ctctttctcc tgcgaagccc ataacgccaa gggggtcacc acatcccgca 721 cagccaccat cacagtgctc ccccagcagc cccgtaacct ccacctggtc tcccgccaac 781 ccacggagct ggaggtggct tggactccag gcctgagcgg catctacccc ctgacccact 841 gcaccctgca ggctgtgctg tcagacgatg ggatgggcat ccaggcggga gaaccagacc 901 ccccagagga gcccctcacc tcgcaagcat ccgtgccccc ccatcagctt cggctaggca 961 gcctccatcc tcacacccct tatcacatcc gcgtggcatg caccagcagc cagggcccct 1021 catcctggac ccactggctt cctgtggaga cgccggaggg agtgcccctg ggcccccctg 1081 agaacattag tgctacgcgg aatgggagcc aggccttcgt gcattggcaa gagccccggg 1141 cgcccctgca gggtaccctg ttagggtacc ggctggcgta tcaaggccag gacaccccag 1201 aggtgctaat ggacataggg ctaaggcaag aggtgaccct ggagctgcag ggggacgggt 1261 ctgtgtccaa tctgacagtg tgtgtggcag cctacactgc tgctggggat ggaccctgga 1321 gcctcccagt acccctggag gcctggcgcc cagggcaagc acagccagtc caccagctgg 1381 tgaaggaacc ttcaactcct gccttctcgt ggccctggtg gtatgtactg ctaggagcag 1441 tcgtggccgc tgcctgtgtc ctcatcttgg ctctcttcct tgtccaccgg cgaaagaagg 1501 agacccgtta tggagaagtg tttgaaccaa cagtggaaag aggtgaactg gtagtcaggt 1561 accgcgtgcg caagtcctac agtcgtcgga ccactgaagc taccttgaac agcctgggca 1621 tcagtgaaga gctgaaggag aagctgcggg atgtgatggt ggaccggcac aaggtggccc 1681 tggggaagac tctgggagag ggagagtttg gagctgtgat ggaaggccag ctcaaccagg 1741 acgactccat cctcaaggtg gctgtgaaga cgatgaagat tgccatctgc acgaggtcag 1801 agctggagga tttcctgagt gaagcggtct gcatgaagga atttgaccat cccaacgtca 1861 tgaggctcat cggtgtctgt ttccagggtt ctgaacgaga gagcttccca gcacctgtgg 1921 tcatcttacc tttcatgaaa catggagacc tacacagctt cctcctctat tcccggctcg 1981 gggaccagcc agtgtacctg cccactcaga tgctagtgaa gttcatggca gacatcgcca 2041 gtggcatgga gtatctgagt accaagagat tcatacaccg ggacctggcg gccaggaact 2101 gcatgctgaa tgagaacatg tccgtgtgtg tggcggactt cgggctctcc aagaagatct 2161 acaatgggga ctactaccgc cagggacgta tcgccaagat gccagtcaag tggattgcca 2221 ttgagagtct agctgaccgt gtctacacca gcaagagcga tgtgtggtcc ttcggggtga 2281 caatgtggga gattgccaca agaggccaaa ccccatatcc gggcgtggag aacagcgaga 2341 tttatgacta tctgcgccag ggaaatcgcc tgaagcagcc TGCGGACTGT CTGGATGGAC 2401 TGTATGCCTT GATGTCGCGG TGCTGGGAGC TAAATCCCCA GGACCGGCCA AGTTTTACAG 2461 AGCTGCGGGA AGATTTGGAG AACACACTGA AGGCCTTGCC TCCTGCCCAG GAGCCTGACG 2521 AAATCCTCTA TGTCAACATG GATGAGGGTG GAGGTTATCC TGAACCCCCT GGAGCTGCAG 2581 GAGGAGCTGA CCCCCCAACC CAGCCAGACC CTAAGGATTC CTGTAGCTGC CTCACTGCGG 2641 CTGAGGTCCA TCCTGCTGGA CGCTATGTCC TCTGCCCTTC CACAACCCCT AGCCCCGCTC 2701 AGCCTGCTGA TAGGGGCTCC CCAGCAGCCC CAGGGCAGGA GGATGGTGCC TAGCCAACTT 2761 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2821 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2881 CTTGTGGAAA GGACGAGTAC GCGAAGCTCC CAGGAGAAGC ACGCGTTAAG TCgacaatca 2941 acctctggat tacaaaattt gtgaaagatt