Construct: ORF TRCN0000481320
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001185.2_s317c1
- Derived from:
- ccsbBroadEn_13548
- DNA Barcode:
- AAGGAACGAACGTCACCAATACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC66 (285331)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481320
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285331 | CCDC66 | coiled-coil domain containi... | NM_001012506.5 | 90.8% | 90.7% | (many diffs) |
| 2 | human | 285331 | CCDC66 | coiled-coil domain containi... | NM_001353152.1 | 90.8% | 90.7% | (many diffs) |
| 3 | human | 285331 | CCDC66 | coiled-coil domain containi... | NM_001353153.1 | 90.8% | 90.7% | (many diffs) |
| 4 | human | 285331 | CCDC66 | coiled-coil domain containi... | NM_001353151.1 | 90.7% | 90.6% | (many diffs) |
| 5 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_011533616.3 | 90.2% | 90.1% | (many diffs) |
| 6 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_011533617.2 | 90.2% | 90.1% | (many diffs) |
| 7 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_024453462.1 | 90.2% | 90.1% | (many diffs) |
| 8 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_011533614.2 | 90.1% | 90% | (many diffs) |
| 9 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_011533615.2 | 90.1% | 90% | (many diffs) |
| 10 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_017006230.2 | 90.1% | 90% | (many diffs) |
| 11 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_024453463.1 | 89.4% | 89.3% | (many diffs) |
| 12 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_011533619.2 | 89.2% | 89.1% | (many diffs) |
| 13 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_024453464.1 | 89.2% | 89.1% | (many diffs) |
| 14 | human | 285331 | CCDC66 | coiled-coil domain containi... | NM_001141947.3 | 87.5% | 87.4% | (many diffs) |
| 15 | human | 285331 | CCDC66 | coiled-coil domain containi... | NM_001353147.1 | 87% | 86.8% | (many diffs) |
| 16 | human | 285331 | CCDC66 | coiled-coil domain containi... | NM_001353148.1 | 86.9% | 86.8% | (many diffs) |
| 17 | human | 285331 | CCDC66 | coiled-coil domain containi... | NM_001353149.1 | 86.8% | 86.7% | (many diffs) |
| 18 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_005265082.4 | 86.2% | 86.1% | (many diffs) |
| 19 | human | 285331 | CCDC66 | coiled-coil domain containi... | NM_001353150.1 | 85.1% | 85% | (many diffs) |
| 20 | human | 285331 | CCDC66 | coiled-coil domain containi... | NM_001353154.1 | 83% | 83% | (many diffs) |
| 21 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_005265083.4 | 82.5% | 82.4% | (many diffs) |
| 22 | human | 285331 | CCDC66 | coiled-coil domain containi... | NM_001353155.1 | 82.3% | 82.2% | (many diffs) |
| 23 | human | 285331 | CCDC66 | coiled-coil domain containi... | NR_148370.1 | 79% | (many diffs) | |
| 24 | human | 285331 | CCDC66 | coiled-coil domain containi... | NR_024460.2 | 78% | (many diffs) | |
| 25 | human | 285331 | CCDC66 | coiled-coil domain containi... | NR_148369.1 | 77.9% | (many diffs) | |
| 26 | human | 285331 | CCDC66 | coiled-coil domain containi... | NR_148368.1 | 77.9% | (many diffs) | |
| 27 | human | 285331 | CCDC66 | coiled-coil domain containi... | NR_148377.1 | 77.3% | (many diffs) | |
| 28 | human | 285331 | CCDC66 | coiled-coil domain containi... | NM_001353156.1 | 77.1% | 76.3% | (many diffs) |
| 29 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_024453468.1 | 77.1% | 76.3% | (many diffs) |
| 30 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_017006236.1 | 76.5% | 75.8% | (many diffs) |
| 31 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_017006237.2 | 76.5% | 75.8% | (many diffs) |
| 32 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_017006238.1 | 76.5% | 75.8% | (many diffs) |
| 33 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_017006239.1 | 76.5% | 75.8% | (many diffs) |
| 34 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_024453465.1 | 76.5% | 75.8% | (many diffs) |
| 35 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_024453466.1 | 76.5% | 75.8% | (many diffs) |
| 36 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_024453467.1 | 76.5% | 75.8% | (many diffs) |
| 37 | human | 285331 | CCDC66 | coiled-coil domain containi... | NR_148375.1 | 76.4% | (many diffs) | |
| 38 | human | 285331 | CCDC66 | coiled-coil domain containi... | NR_148366.1 | 76% | (many diffs) | |
| 39 | human | 285331 | CCDC66 | coiled-coil domain containi... | NR_148376.1 | 75.9% | (many diffs) | |
| 40 | human | 285331 | CCDC66 | coiled-coil domain containi... | NR_148367.1 | 75.9% | (many diffs) | |
| 41 | human | 285331 | CCDC66 | coiled-coil domain containi... | XR_001740115.2 | 75.8% | (many diffs) | |
| 42 | human | 285331 | CCDC66 | coiled-coil domain containi... | NR_148378.1 | 74.7% | (many diffs) | |
| 43 | human | 285331 | CCDC66 | coiled-coil domain containi... | NR_148371.1 | 74.1% | (many diffs) | |
| 44 | human | 285331 | CCDC66 | coiled-coil domain containi... | NR_148372.1 | 73.7% | (many diffs) | |
| 45 | human | 285331 | CCDC66 | coiled-coil domain containi... | NR_148374.1 | 73.3% | (many diffs) | |
| 46 | human | 285331 | CCDC66 | coiled-coil domain containi... | NR_148373.1 | 73.3% | (many diffs) | |
| 47 | human | 285331 | CCDC66 | coiled-coil domain containi... | NM_001353158.1 | 72% | 71.3% | (many diffs) |
| 48 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_017006242.2 | 72% | 71.3% | (many diffs) |
| 49 | human | 285331 | CCDC66 | coiled-coil domain containi... | NM_001353160.1 | 70.1% | 70% | 0_1ins741;56A>G;287G>A |
| 50 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_017006243.2 | 70.1% | 70% | 0_1ins741;56A>G;287G>A |
| 51 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_024453469.1 | 69.6% | 69.5% | (many diffs) |
| 52 | human | 285331 | CCDC66 | coiled-coil domain containi... | XM_024453470.1 | 69.6% | 69.5% | (many diffs) |
| 53 | human | 285331 | CCDC66 | coiled-coil domain containi... | XR_001740111.2 | 62.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2562
- ORF length:
- 2493
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagaagact agaaacaccg taaatacatc tctagtaggt aaacagaagc 121 ctcacaaaaa acacatcaca gctgaaaaca tgaagagcag tttggtgtgt ctaacacaag 181 accaactaca acagattttg atgactgtaa accaaggaaa tagatctctt tccctgactg 241 agaatggaaa ggaggcaaaa agtcaatata gtctatattt aaacagtatt tctaatcagc 301 caaaggatga gaacattatg ggattattca aaaaaactga aatggtttca tctgtcccag 361 ctgaaaataa atctgtctta aatgaacatc aggagacatc taaacagtgt gagcaaaaaa 421 ttgccataga gaatgaatgg aaaccagctg atatattcag tactctgggg gaaagggaat 481 gtgatagaag ttcgttggaa gcaaaaaaag cccagtggag gaaagagcta gatgaacagg 541 ttgctttaaa gaagaaagaa aaagaagttt ctgaaaaatg gaatgatcct tggaaaaaat 601 ctgaaagtga taaaataata tgggaaaaac atcaaattct tgaccaatct agggaaacag 661 tactgctgga gcaccctttc agtgctgtga aacaagaact gcagagaaaa tggattgaag 721 agttgaataa gcaaatagaa gatgaccgtc aaagaaaaat agaggaaaaa attatatatt 781 caaagggtga ggaacatgac agatgggcaa tgcactttga ttcattaaag agttatcctg 841 gttctcaatc tcagctgttc tctcggtcaa cacacaaaca acctgagtac ttctgtgtct 901 ctcctgacac tcaggagctg gctgatgtca gcagtgtttg tacacctaca accggaagcc 961 aggttgaacc ttcagaggag gagcatatag caaaacctat taaggatgtg gttatggcaa 1021 acagtaagaa aacaaacttt ctccgttcta tgactgctct cttggaccca gctcagattg 1081 aggaacgaga cagacaacga caaaaacaat tagagcatca gaaagccatc actgcccagg 1141 tagaagagaa gcgcaggaag aaacaactgg aggaagagca aagaaagaag gaagaacaag 1201 aagaggagct tcgcttagca caggaacgtg aagagatgca gaaacagtat gaagaagaca 1261 tacttaagca aaaacaaaag gaagaaatca tgactctcaa gacaaatgag ctattccaga 1321 caatgcagcg agcacaggaa ctggcacaga gactaaaaca agaacaaaga atccgagaat 1381 tggcgcaaaa gggacatgac acttctagac tgattaaaaa tcttggtgtt gatacaatac 1441 aaatggaata taatgcatct aacatttcaa attcaagaca tgattctgat gaaatcagtg 1501 gtaaaatgaa tacatatatg aattctacga cttctaagaa ggatactggt gtgcaaacag 1561 atgacttaaa tataggaata ttcaccaatg cagaatcaca ttgtggatca ttaatggaga 1621 gggacatcac aaattgttca tctcctgaga tttcggcaga acttattgga cagtttagca 1681 ccaagaaaaa caagcaagaa ctaactcagg ataaaggagc cagcttagaa aaagaaaaca 1741 atcggtgtaa tgaccagtgt aatcagttca caagaataga gaaacaaaca aaacacatga 1801 agaaatatcc taaaaggcct gattggaata taaataagcc acctaaaagg tatattccag 1861 catcagaaaa gtaccctaaa cagcttcaaa agcagagaga agaaaaaaaa gtaaggaggc 1921 agatggaatt gcttcatttg gtagaaaaaa ataatcctgg gcacctctct caaaacagag 1981 gcatttcacc agaaattttt cattcatctc atcaagaaac ggagtcaaag ttgaggtggc 2041 atctagtcaa aaaggaagaa gagcctctga atattcattc attcagcaag gaaaggtctc 2101 catcatcacc agttccagta gtgaaaaaca gaacccaaca aactcaaaat acattacatt 2161 taccactaaa aaacagtagc tatgagagag agaatttgat ctcaggaagt aatcaaacag 2221 aattatcatc tgggatttct gaaTCATCCC ATTTTATTCC GTATGTTCGA ACAAATGAGA 2281 TCTATTACCT TGATCCCGAT GCACCATTGT CTGGGCCTTC AACCCAGGAC CCTCAGTACC 2341 AAAATTCACA AGACTGTGGC CAAAAACGAC AGCTATTTGA TTCTGACTGT GTCAGGGATC 2401 CACTTCTTAA TCCTAACATG GTGAAAAATA GGGATCGACA GCAAGCAATC CTTAAGGGAC 2461 TTTCAGAACT GAGACAGGGC CTTCTCCAGA AGCAAAAGGA GTTGGAAAGT AGTCTCCTGC 2521 CTTTAGCTGA AAATCAAGAA GAGAGTTTTG GTTCTTCATT TTTGCCAACT TTCTTGTACA 2581 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2641 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2701 AGGACGAAAG GAACGAACGT CACCAATACC AACGCGTTAA GTCgacaatc aacctctgga 2761 ttacaaaatt tgtgaaagat t