Transcript: Human XR_001740111.2

PREDICTED: Homo sapiens coiled-coil domain containing 66 (CCDC66), transcript variant X24, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CCDC66 (285331)
Length:
2265
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001740111.2
NBCI Gene record:
CCDC66 (285331)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001740111.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000445479 AGACTAGAAACACCGTAAATA pLKO_005 439 3UTR 100% 15.000 21.000 N CCDC66 n/a
2 TRCN0000134816 CAAATAGAAGATGACCGTCAA pLKO.1 1095 3UTR 100% 4.050 5.670 N CCDC66 n/a
3 TRCN0000135179 CTGAGATTTCGGCAGAACTTA pLKO.1 2012 3UTR 100% 5.625 3.938 N CCDC66 n/a
4 TRCN0000136134 GCTGTGAAACAAGAACTGCAA pLKO.1 1047 3UTR 100% 2.640 1.848 N CCDC66 n/a
5 TRCN0000450143 ATGGAATATAATGCATCTAAC pLKO_005 1810 3UTR 100% 10.800 6.480 N CCDC66 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001740111.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13547 pDONR223 100% 74.3% None (many diffs) n/a
2 ccsbBroad304_13547 pLX_304 0% 74.3% V5 (many diffs) n/a
3 TRCN0000467805 CCGAGTCAACCCACACGCTCAACA pLX_317 16.3% 74.3% V5 (many diffs) n/a
4 ccsbBroadEn_13548 pDONR223 100% 62.3% None (many diffs) n/a
5 ccsbBroad304_13548 pLX_304 0% 62.3% V5 (many diffs) n/a
6 TRCN0000481320 AAGGAACGAACGTCACCAATACCA pLX_317 13% 62.3% V5 (many diffs) n/a
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