Construct: ORF TRCN0000481436
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001978.1_s317c1
- Derived from:
- ccsbBroadEn_14514
- DNA Barcode:
- GCTCGTTTACTAGTATTTTTAATC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC644936 (644936)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481436
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60 | ACTB | actin beta | NM_001101.5 | 51.7% | 45% | (many diffs) |
| 2 | human | 71 | ACTG1 | actin gamma 1 | NM_001199954.2 | 50% | 45% | (many diffs) |
| 3 | human | 71 | ACTG1 | actin gamma 1 | NM_001614.5 | 50% | 45% | (many diffs) |
| 4 | human | 644936 | LOC644936 | actin beta pseudogene | NR_004845.2 | 47% | 1_141del;789delC;799_1394del | |
| 5 | human | 71 | ACTG1 | actin gamma 1 | NR_037688.3 | 31.7% | (many diffs) | |
| 6 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_017004743.2 | 29.1% | 24.9% | (many diffs) |
| 7 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_017004734.2 | 28.9% | 24.5% | (many diffs) |
| 8 | human | 728378 | POTEF | POTE ankyrin domain family ... | XM_017004835.1 | 28.8% | 24.3% | (many diffs) |
| 9 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_017004742.1 | 28.3% | 24.2% | (many diffs) |
| 10 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_017004733.1 | 28.2% | 23.8% | (many diffs) |
| 11 | human | 728378 | POTEF | POTE ankyrin domain family ... | XM_017004834.1 | 28% | 23.7% | (many diffs) |
| 12 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_011511216.2 | 19.8% | 16.6% | (many diffs) |
| 13 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_017004159.2 | 19.6% | 16.4% | (many diffs) |
| 14 | human | 653781 | POTEJ | POTE ankyrin domain family ... | NM_001277083.2 | 18.4% | 15.7% | (many diffs) |
| 15 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_011511683.2 | 18.2% | 15.6% | (many diffs) |
| 16 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_017004741.2 | 18.2% | 15.6% | (many diffs) |
| 17 | human | 653269 | POTEI | POTE ankyrin domain family ... | NM_001277406.2 | 17.7% | 14.9% | (many diffs) |
| 18 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_024453046.1 | 17.7% | 14.9% | (many diffs) |
| 19 | human | 445582 | POTEE | POTE ankyrin domain family ... | NM_001083538.2 | 17.6% | 14.7% | (many diffs) |
| 20 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_017004161.2 | 17.6% | 14.7% | (many diffs) |
| 21 | human | 728378 | POTEF | POTE ankyrin domain family ... | NM_001099771.2 | 17.6% | 14.8% | (many diffs) |
| 22 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_011511670.2 | 17.5% | 14.8% | (many diffs) |
| 23 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_017004732.2 | 17.5% | 14.8% | (many diffs) |
| 24 | human | 728378 | POTEF | POTE ankyrin domain family ... | XM_017004833.1 | 17.4% | 14.7% | (many diffs) |
| 25 | human | 653269 | POTEI | POTE ankyrin domain family ... | NM_001371926.1 | 17.3% | 14.6% | (many diffs) |
| 26 | mouse | 11461 | Actb | actin, beta | NM_007393.5 | 49.4% | 45% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 726
- ORF length:
- 657
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggtcacca acactgtgcc catctatgaa gggtacaccc ctcccccaca 121 ccatcctgtg tctggacctg actggccgga acctgactga ctacctcatg aagatcctca 181 cccagtgtgg ctacagcttc accaccacag tcatgcagga aattgtgtgt gacatcaaga 241 aaaggctgtg ctacatcccc ctggactttg agcaggagat ggccatggtg ggctccagcT 301 CCTCCCTGGA GAAGAGCTAC AAGCTGCCCA ATGGCCAGGT CATCACCATT AGCAACAAGT 361 GGTTCTGCTG CCCCGAGGCA CTCTTCCAAA CTTCCTTCGT GGGCATGGAA TCCTGTGGCA 421 TCCACGAAAC TACCTTCAAC TCCATCATGA AGTCGGATGT GGACATCTAC AAAGACCTGT 481 ATGCCAACAC AGTGCTGTCT GGCAGCACCA CCATGTACCC TAGCATCACC AACAGGATGC 541 AGAAGGAGAT TACCGCCCTG GCGCCCAGCG CAATGAAGAT CAAGATCATT GCTCCTCCTG 601 AGTGCAAGTA CTCTGTGTGG ATCAGAGGCT CCATCCTGGC CTCGCTGTCC ACCTTCCAGC 661 AGATGTGGAT CAGCAAGCAG GAGTACAGTG AGTCCAGCCC CTCCATCGTC CACCGAAATG 721 CTTCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT 781 CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC 841 TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCTCGTTTAC TAGTATTTTT AATCACGCGT 901 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt