Construct: ORF TRCN0000487677

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020423.1_s317c1
DNA Barcode:
GGAGAAATTATCCTCAACCATTAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
AKT3 (10000)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487677
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10000 AKT3 AKT serine/threonine kinase 3 NM_001370074.1 100% 100%
2 human 10000 AKT3 AKT serine/threonine kinase 3 NM_005465.7 100% 100%
3 human 10000 AKT3 AKT serine/threonine kinase 3 NM_001206729.1 95.8% 94.3% (many diffs)
4 human 10000 AKT3 AKT serine/threonine kinase 3 NM_181690.2 95.8% 94.3% (many diffs)
5 human 10000 AKT3 AKT serine/threonine kinase 3 XM_024446000.1 89.4% 88.9% (many diffs)
6 human 10000 AKT3 AKT serine/threonine kinase 3 XM_016999985.1 87.2% 87.2% 0_1ins183
7 human 10000 AKT3 AKT serine/threonine kinase 3 XM_024446892.1 60.4% 58% (many diffs)
8 human 10000 AKT3 AKT serine/threonine kinase 3 XM_024447938.1 50.5% 49.2% (many diffs)
9 human 10000 AKT3 AKT serine/threonine kinase 3 XM_011544014.2 50.2% 42.1% (many diffs)
10 mouse 23797 Akt3 thymoma viral proto-oncogene 3 NM_011785.3 94% 99.5% (many diffs)
11 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_006496818.3 94% 99.5% (many diffs)
12 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_006496819.3 94% 99.5% (many diffs)
13 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_011238805.2 84.4% 85.6% (many diffs)
14 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_017320418.1 81.6% 86.8% (many diffs)
15 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_006496821.3 54.1% 53.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
84
ORF end:
1521
ORF length:
1437
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggctccgc ggcccccttc accatgagcg atgttaccat tgtgaaagaa ggttgggttc
121agaagagggg agaatatata aaaaactgga ggccaagata cttccttttg aagacagatg
181gctcattcat aggatataaa gagaaacctc aagatgtgga tttaccttat cccctcaaca
241acttttcagt ggcaaaatgc cagttaatga aaacagaacg accaaagcca aacacattta
301taatcagatg tctccagtgg actactgtta tagagagaac atttcatgta gatactccag
361aggaaaggga agaatggaca gaagctatcc aggctgtagc agacagactg cagaggcaag
421aagaggagag aatgaattgt agtccaactt cacaaattga taatatagga gaggaagaga
481tggatgcctc tacaacccat cataaaagaa agacaatgaa tgattttgac tatttgaaac
541tactaggtaa aggcactttt gggaaagtta ttttggttcg agagaaggca agtggaaaat
601actatgctat gaagattctg aagaaagaag tcattattgc aaaggatgaa gtggcacaca
661ctctaactga aagcagagta ttaaagaaca ctagacatcc ctttttaaca tccttgaaat
721attccttcca gacaaaagac cgtttgtgtt ttgtgatgga atatgttaat gggggcgagc
781tgtttttcca tttgtcgaga gagcgggtgt tctctgagga ccgcacacgt ttctatggtg
841cagaaattgt ctctgccttg gactatctac attccggaaa gattgtgtac cgtgatctca
901agttggagaa tctaatgctg gacaaagatg gccacataaa aattacagat tttggacttt
961gcaaagaagg gatcacagat gcagccacca tgaagacatt ctgtggcact ccagaatatc
1021tggcaccaga ggtgttagaa gataatgact atggccgagc agtagactgg tggggcctag
1081gggttgtcat gtatgaaatg atgtgtggga ggttaccttt ctacaaccag gaccatgaga
1141aactttttga attaatatta atggaagaca ttaaatttcc tcgaacactc tcttcagatg
1201caaaatcatt gctttcaggg ctcttgataa aggatccaaa taaacgcctt ggtggaggac
1261cagatgatgc aaaagaaatt atgagacaca gtttcttctc tggagtaaac tggcaagatg
1321tatatgataa aaagcttgta cctcctttta aacctcaagt aacatctgag acagatacta
1381gatattttga tgaagaattt acagctcaga ctattacaaT AACACCACCT GAAAAATATG
1441ATGAGGATGG TATGGACTGC ATGGACAATG AGAGGCGGCC GCATTTCCCT CAATTTTCCT
1501ACTCTGCAAG TGGACGAGAA TAATCTAGAA AGGGTGGGCG CGCCGACCCA GCTTTCTTGT
1561ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1621AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1681GAAAGGACGA GGAGAAATTA TCCTCAACCA TTACACGCGT TAAGTCgaca atcaacctct
1741ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)