Construct: ORF TRCN0000487794
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020519.1_s317c1
- DNA Barcode:
- GACACCTTGAAGTCTTTTATAATT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SYK (6850)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487794
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001135052.4 | 100% | 100% | |
| 2 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001174168.3 | 100% | 100% | |
| 3 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001174167.3 | 96.3% | 96.3% | 846_914del |
| 4 | human | 6850 | SYK | spleen associated tyrosine ... | NM_003177.7 | 96.3% | 96.3% | 846_914del |
| 5 | human | 6850 | SYK | spleen associated tyrosine ... | XM_005252147.4 | 96.3% | 96.3% | 846_914del |
| 6 | human | 6850 | SYK | spleen associated tyrosine ... | XM_011518946.3 | 96.3% | 96.3% | 846_914del |
| 7 | human | 6850 | SYK | spleen associated tyrosine ... | XR_001746370.2 | 36.2% | 1_166del;1012_1117del;2109_5060del | |
| 8 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_006516897.4 | 84.2% | 92.1% | (many diffs) |
| 9 | mouse | 20963 | Syk | spleen tyrosine kinase | NM_001198977.1 | 81.9% | 88.8% | (many diffs) |
| 10 | mouse | 20963 | Syk | spleen tyrosine kinase | NM_011518.2 | 81.9% | 88.8% | (many diffs) |
| 11 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_006516895.4 | 81.9% | 88.8% | (many diffs) |
| 12 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_017315471.2 | 48.6% | 53.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1908
- ORF length:
- 1836
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccagc agcggcatgg ctgacagcgc caaccacctg cccttctttt 121 tcggcaacat cacccgggag gaggcagaag attacctggt ccaggggggc atgagtgatg 181 ggctttattt gctgcgccag agccgcaact acctgggtgg cttcgccctg tccgtggccc 241 acgggaggaa ggcacaccac tacaccatcg agcgggagct gaatggcacc tacgccatcg 301 ccggtggcag gacccatgcc agccccgccg acctctgcca ctaccactcc caggagtctg 361 atggcctggt ctgcctcctc aagaagccct tcaaccggcc ccaaggggtg cagcccaaga 421 ctgggccctt tgaggatttg aaggaaaacc tcatcaggga atatgtgaag cagacatgga 481 acctgcaggg tcaggctctg gagcaggcca tcatcagtca gaagcctcag ctggagaagc 541 tgatcgctac cacagcccat gaaaaaatgc cttggttcca tggaaaaatc tctcgggaag 601 aatctgagca aattgtcctg ataggatcaa agacaaatgg aaagttcctg atccgagcca 661 gagacaacaa cggctcctac gccctgtgcc tgctgcacga agggaaggtg ctgcactatc 721 gcatcgacaa agacaagaca gggaagctct ccatccccga gggaaagaag ttcgacacgc 781 tctggcagct agtcgagcat tattcttata aagcagatgg tttgttaaga gttcttactg 841 tcccatgtca aaaaatcggc acacagggaa atgttaattt tggaggccgt ccacaacttc 901 caggttccca tcctgcgtcc tcccctgccc aagggaaccg gcaagagagt actgtgtcat 961 tcaatccgta tgagccagaa cttgcaccct gggctgcaga caaaggcccc cagagagaag 1021 ccctacccat ggacacagag gtgtacgaga gcccctacgc ggaccccgag gagatcaggc 1081 ccaaggaggt ttacctggac cgaaagctgc tgacgctgga agacaaagaa ctgggctctg 1141 gtaattttgg aactgtgaaa aagggctact accaaatgaa aaaagttgtg aaaaccgtgg 1201 ctgtgaaaat actgaaaaac gaggccaatg accccgctct taaagatgag ttattagcag 1261 aagcaaatgt catgcagcag ctggacaacc cgtacatcgt gcggatgatc gggatatgcg 1321 aggccgagtc ctggatgctg gttatggaga tggcagaact tggtcccctc aataagtatt 1381 tgcagcagaa cagacatgtc aaggataaga acatcataga actggttcat caggtttcca 1441 tgggcatgaa gtacttggag gagagcaatt ttgtgcacag agatctggct gcaagaaatg 1501 tgttgctagt tacccaacat tacgccaaga tcagtgattt cggactttcc aaagcactgc 1561 gtgctgatga aaactactac aaggcccaga cccatggaaa gtggcctgtc aagtggtacg 1621 ctccggaatg catcaactac tacaagttct ccagcaaaag cgatgtctgg agctttggag 1681 tgttgatgtg ggaagcaTTC TCCTATGGGC AGAAGCCATA TCGAGGGATG AAAGGAAGTG 1741 AAGTCACCGC TATGTTAGAG AAAGGAGAGC GGATGGGGTG CCCTGCAGGG TGTCCAAGAG 1801 AGATGTACGA TCTCATGAAT CTGTGCTGGA CATACGATGT GGAAAACAGG CCCGGATTCG 1861 CAGCAGTGGA ACTGCGGCTG CGCAATTACT ACTATGACGT GGTGAACTAG AACCCAGCTT 1921 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1981 CTACGTAGTA ATGAACTAGC CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2041 CTTGTGGAAA GGACGAGACA CCTTGAAGTC TTTTATAATT ACGCGTTAAG TCgacaatca 2101 acctctggat tacaaaattt gtgaaagatt