Construct: ORF TRCN0000487969

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020648.1_s317c1
DNA Barcode:
TCACTCTGCATCGTATCCGTTGCT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
IKBKB (3551)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487969
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3551 IKBKB inhibitor of nuclear factor... NM_001556.3 100% 100%
2 human 3551 IKBKB inhibitor of nuclear factor... NM_001190720.2 95.2% 93.9% (many diffs)
3 human 3551 IKBKB inhibitor of nuclear factor... XM_005273492.4 93.3% 93.1% 2114_2115insTGAAGAACTGGTGGCTGAA;2117C>A;2118_2119ins131
4 human 3551 IKBKB inhibitor of nuclear factor... XM_005273490.3 92.5% 90% (many diffs)
5 human 3551 IKBKB inhibitor of nuclear factor... XM_011544517.2 92.5% 90% (many diffs)
6 human 3551 IKBKB inhibitor of nuclear factor... NM_001242778.2 92.1% 91.2% 0_1ins82;21_22ins95
7 human 3551 IKBKB inhibitor of nuclear factor... XM_005273494.3 88.8% 87.2% (many diffs)
8 human 3551 IKBKB inhibitor of nuclear factor... XM_011544518.2 86.3% 81.9% (many diffs)
9 human 3551 IKBKB inhibitor of nuclear factor... XM_005273491.5 85.2% 82% (many diffs)
10 human 3551 IKBKB inhibitor of nuclear factor... XM_011544519.2 85.2% 82% (many diffs)
11 human 3551 IKBKB inhibitor of nuclear factor... XM_011544520.2 79.9% 72.7% (many diffs)
12 human 3551 IKBKB inhibitor of nuclear factor... XM_005273493.4 79.5% 74.5% (many diffs)
13 human 3551 IKBKB inhibitor of nuclear factor... XM_005273495.2 66.5% 61.3% (many diffs)
14 human 3551 IKBKB inhibitor of nuclear factor... XM_005273496.4 66.5% 61.3% (many diffs)
15 human 3551 IKBKB inhibitor of nuclear factor... XM_005273498.4 66.5% 61.3% (many diffs)
16 human 3551 IKBKB inhibitor of nuclear factor... XM_011544521.2 66.5% 61.3% (many diffs)
17 human 3551 IKBKB inhibitor of nuclear factor... XM_011544522.2 58% 55.9% (many diffs)
18 human 3551 IKBKB inhibitor of nuclear factor... XM_017013396.1 58% 55.9% (many diffs)
19 human 3551 IKBKB inhibitor of nuclear factor... NR_033818.2 56.7% 1_186del;575_630del;2511_3994del
20 human 3551 IKBKB inhibitor of nuclear factor... NR_040009.2 56.2% 1_186del;1309_1310ins53;2402_3885del
21 human 3551 IKBKB inhibitor of nuclear factor... NR_033819.2 56% 1_186del;1702_1703ins62;2393_3876del
22 human 3551 IKBKB inhibitor of nuclear factor... XR_001745530.2 53.8% (many diffs)
23 human 3551 IKBKB inhibitor of nuclear factor... XR_949402.3 46.8% (many diffs)
24 mouse 16150 Ikbkb inhibitor of kappaB kinase ... NM_001159774.1 87% 92.4% (many diffs)
25 mouse 16150 Ikbkb inhibitor of kappaB kinase ... XM_006509019.3 85.9% 90.6% (many diffs)
26 mouse 16150 Ikbkb inhibitor of kappaB kinase ... NM_010546.2 84.9% 90.7% (many diffs)
27 mouse 16150 Ikbkb inhibitor of kappaB kinase ... XM_006509020.3 81.8% 87.5% (many diffs)
28 mouse 16150 Ikbkb inhibitor of kappaB kinase ... XM_006509021.2 63.2% 64.2% (many diffs)
29 mouse 16150 Ikbkb inhibitor of kappaB kinase ... XM_006509022.3 62% 65.3% (many diffs)
30 mouse 16150 Ikbkb inhibitor of kappaB kinase ... XM_006509023.3 48.5% 51.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
99
ORF end:
2367
ORF length:
2268
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggctccgc ggccgccccc ttcacccaat tgatcagtat gagctggtca ccttccctga
121caacgcagac atgtggggcc tgggaaatga aagagcgcct tgggacaggg ggatttggaa
181atgtcatccg atggcacaat caggaaacag gtgagcagat tgccatcaag cagtgccggc
241aggagctcag cccccggaac cgagagcggt ggtgcctgga gatccagatc atgagaaggc
301tgacccaccc caatgtggtg gctgcccgag atgtccctga ggggatgcag aacttggcgc
361ccaatgacct gcccctgctg gccatggagt actgccaagg aggagatctc cggaagtacc
421tgaaccagtt tgagaactgc tgtggtctgc gggaaggtgc catcctcacc ttgctgagtg
481acattgcctc tgcgcttaga taccttcatg aaaacagaat catccatcgg gatctaaagc
541cagaaaacat cgtcctgcag caaggagaac agaggttaat acacaaaatt attgacctag
601gatatgccaa ggagctggat cagggcagtc tttgcacatc attcgtgggg accctgcagt
661acctggcccc agagctactg gagcagcaga agtacacagt gaccgtcgac tactggagct
721tcggcaccct ggcctttgag tgcatcacgg gcttccggcc cttcctcccc aactggcagc
781ccgtgcagtg gcattcaaaa gtgcggcaga agagtgaggt ggacattgtt gttagcgaag
841acttgaatgg aacggtgaag ttttcaagct ctttacccta ccccaataat cttaacagtg
901tcctggctga gcgactggag aagtggctgc aactgatgct gatgtggcac ccccgacaga
961ggggcacgga tcccacgtat gggcccaatg gctgcttcaa ggccctggat gacatcttaa
1021acttaaagct ggttcatatc ttgaacatgg tcacgggcac catccacacc taccctgtga
1081cagaggatga gagtctgcag agcttgaagg ccagaatcca acaggacacg ggcatcccag
1141aggaggacca ggagctgctg caggaagcgg gcctggcgtt gatccccgat aagcctgcca
1201ctcagtgtat ttcagacggc aagttaaatg agggccacac attggacatg gatcttgttt
1261ttctctttga caacagtaaa atcacctatg agactcagat ctccccacgg ccccaacctg
1321aaagtgtcag ctgtatcctt caagagccca agaggaatct cgccttcttc cagctgagga
1381aggtgtgggg ccaggtctgg cacagcatcc agaccctgaa ggaagattgc aaccggctgc
1441agcagggaca gcgagccgcc atgatgaatc tcctccgaaa caacagctgc ctctccaaaa
1501tgaagaattc catggcttcc atgtctcagc agctcaaggc caagttggat ttcttcaaaa
1561ccagcatcca gattgacctg gagaagtaca gcgagcaaac cgagtttggg atcacatcag
1621ataaactgct gctggcctgg agggaaatgg agcaggctgt ggagctctgt gggcgggaga
1681acgaagtgaa actcctggta gaacggatga tggctctgca gaccgacatt gtggacttac
1741agaggagccc catgggccgg aagcaggggg gaacgctgga cgacctagag gagcaagcaa
1801gggagctgta caggagacta agggaaaaac ctcgagacca gcgaactgag ggtgacagtc
1861aggaaatggt acggctgctg cttcaggcaa ttcagagctt cgagaagaaa gtgcgagtga
1921tctatacgca gctcagtaaa actgtggttt gcaagcagaa ggcgctggaa ctgttgccca
1981aggtggaaga ggtggtgagc ttaatgaatg aggatgagaa gactgttgtc cggctgcagg
2041agaagcggca gaaggagctc tggaatctcc tgaagattgc ttGTAGCAAG GTCCGTGGTC
2101CTGTCAGTGG AAGCCCGGAT AGCATGAATG CCTCTCGACT TAGCCAGCCT GGGCAGCTGA
2161TGTCTCAGCC CTCCACGGCC TCCAACAGCT TACCTGAGCC AGCCAAGAAG AGTGAAGAAC
2221TGGTGGCTGA AGCACATAAC CTCTGCACCC TGCTAGAAAA TGCCATACAG GACACTGTGA
2281GGGAACAAGA CCAGAGTTTC ACGGCCCTAG ACTGGAGCTG GTTACAGACG GAAGAAGAAG
2341AGCACAGCTG CCTGGAGCAG GCCTCATGAA AGCTTAAGGG TGGGCGCGCC GACCCAGCTT
2401TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
2461CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
2521CTTGTGGAAA GGACGATCAC TCTGCATCGT ATCCGTTGCT ACGCGTTAAG TCgacaatca
2581acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)