Construct: ORF TRCN0000488555

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021654.1_s317c1
DNA Barcode:
GGCGAATAATCCAGTCCTGCTCTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PRKAG2 (51422)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488555
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51422 PRKAG2 protein kinase AMP-activate... NM_016203.4 100% 100%
2 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012269.1 99.8% 99.8% 752_753insAGC
3 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516283.1 94.3% 91.2% (many diffs)
4 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516284.1 94.1% 91.1% (many diffs)
5 human 51422 PRKAG2 protein kinase AMP-activate... NM_001040633.1 92.2% 92.2% 0_1ins132
6 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012270.1 92.2% 92.2% 0_1ins132
7 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012271.2 92% 92% 0_1ins132;620_621insAGC
8 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012272.1 92% 92% 0_1ins132;620_621insAGC
9 human 51422 PRKAG2 protein kinase AMP-activate... XM_005250002.4 90.9% 90.1% (many diffs)
10 human 51422 PRKAG2 protein kinase AMP-activate... XM_006716021.2 85.8% 82.4% (many diffs)
11 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516282.1 85.7% 82.3% (many diffs)
12 human 51422 PRKAG2 protein kinase AMP-activate... XM_005250004.4 83.8% 83.1% (many diffs)
13 human 51422 PRKAG2 protein kinase AMP-activate... XM_024446786.1 83.8% 83.1% (many diffs)
14 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012268.2 83.6% 82.9% (many diffs)
15 human 51422 PRKAG2 protein kinase AMP-activate... NM_001363698.1 74.4% 73.7% (many diffs)
16 human 51422 PRKAG2 protein kinase AMP-activate... NM_001304527.1 74.2% 73.6% (many diffs)
17 human 51422 PRKAG2 protein kinase AMP-activate... XM_005250006.5 67.6% 66.2% (many diffs)
18 human 51422 PRKAG2 protein kinase AMP-activate... NM_001304531.1 57.6% 57.6% 0_1ins723
19 human 51422 PRKAG2 protein kinase AMP-activate... NM_024429.1 57.6% 57.6% 0_1ins723
20 human 51422 PRKAG2 protein kinase AMP-activate... XM_024446789.1 57.6% 57.6% 0_1ins723
21 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012276.2 57.4% 57.4% 0_1ins723;29_30insAGC
22 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012277.2 56.1% 55.8% 0_1ins745;2T>G;6_6delCinsGAA
23 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012278.1 54.1% 54.1% 0_1ins783
24 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012279.2 54.1% 54.1% 0_1ins783
25 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012280.2 54.1% 54.1% 0_1ins783
26 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012281.2 54.1% 54.1% 0_1ins783
27 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516285.2 52.1% 51.4% (many diffs)
28 human 51422 PRKAG2 protein kinase AMP-activate... XM_024446787.1 52.1% 51.4% (many diffs)
29 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012274.2 51.9% 51.2% (many diffs)
30 human 51422 PRKAG2 protein kinase AMP-activate... XM_024446788.1 51.9% 51.2% (many diffs)
31 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516286.2 50.7% 49.8% (many diffs)
32 human 51422 PRKAG2 protein kinase AMP-activate... XM_011516287.1 48.9% 48.2% (many diffs)
33 human 51422 PRKAG2 protein kinase AMP-activate... XM_017012275.2 48.9% 48.2% (many diffs)
34 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_006535611.3 89.3% 93.6% (many diffs)
35 mouse 108099 Prkag2 protein kinase, AMP-activat... NM_145401.2 89.1% 93.4% (many diffs)
36 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_006535612.3 82.7% 86.8% (many diffs)
37 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_017320595.1 76.9% 80.3% (many diffs)
38 mouse 108099 Prkag2 protein kinase, AMP-activat... NM_001310480.1 68.8% 69.7% (many diffs)
39 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_006535614.3 65.9% 68.7% (many diffs)
40 mouse 108099 Prkag2 protein kinase, AMP-activat... NM_001170555.1 52.8% 56.7% (many diffs)
41 mouse 108099 Prkag2 protein kinase, AMP-activat... NM_001170556.1 52.7% 56.5% (many diffs)
42 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_011249740.1 51.9% 55.5% (many diffs)
43 mouse 108099 Prkag2 protein kinase, AMP-activat... XM_017320596.1 51.9% 55.3% (many diffs)
44 mouse 108099 Prkag2 protein kinase, AMP-activat... XR_880821.2 40.5% (many diffs)
45 mouse 108099 Prkag2 protein kinase, AMP-activat... XR_389738.3 39.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1776
ORF length:
1707
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gggaagcgcg gttatggaca ccaagaagaa aaaagatgtt tccagccccg
121gcgggagcgg cggcaagaaa aatgccagcc agaagaggcg ttcgctgcgc gtgcacattc
181cggacctgag ctccttcgcc atgccgctcc tggacggaga cctggagggt tccggaaagc
241attcctctcg aaaggtggac agccccttcg gcccgggcag cccctccaaa gggttcttct
301ccagaggccc ccagccccgg ccctccagcc ccatgtctgc acctgtgagg cccaagacca
361gccccggctc tcccaaaacc gtgttcccgt tctcctacca ggagtccccg ccacgctccc
421ctcgacgcat gagcttcagt gggatcttcc gctcctcctc caaagagtct tcccccaact
481ccaaccctgc tacctcgccc gggggcatca ggtttttctc ccgctccaga aaaacctccg
541gcctctcctc ctctccgtca acacccaccc aagtgaccaa gcagcacacg tttcccctgg
601aatcctataa gcacgagcct gaacggttag agaatcgcat ctatgcctcg tcttcccccc
661cggacacagg gcagaggttc tgcccgtctt ccttccagag cccgaccagg cctccactgg
721catcaccgac acactatgct ccctccaaag ccgcggcgct ggcggcggcc ctgggacccg
781cggaagccgg catgctggag aagctggagt tcgaggacga agcagtagaa gactcagaaa
841gtggtgttta catgcgattc atgaggtcac acaagtgtta tgacatcgtt ccaaccagtt
901caaagcttgt tgtctttgat actacattac aagttaaaaa ggccttcttt gctttggtag
961ccaacggtgt ccgagcagcg ccactgtggg agagtaaaaa acaaagtttt gtaggaatgc
1021taacaattac agatttcata aatatactac atagatacta taaatcacct atggtacaga
1081tttatgaatt agaggaacat aaaattgaaa catggaggga gctttattta caagaaacat
1141ttaagccttt agtgaatata tctccagatg caagcctctt cgatgctgta tactccttga
1201tcaaaaataa aatccacaga ttgcccgtta ttgaccctat cagtgggaat gcactttata
1261tacttaccca caaaagaatc ctcaagttcc tccagctttt tatgtctgat atgccaaagc
1321ctgccttcat gaagcagaac ctggatgagc ttggaatagg aacgtaccac aacattgcct
1381tcatacatcc agacactccc atcatcaaag ccttgaacat atttgtGGAA AGACGAATAT
1441CAGCTCTGCC TGTTGTGGAT GAGTCAGGAA AAGTTGTAGA TATTTATTCC AAATTTGATG
1501TAATTAATCT TGCTGCTGAG AAAACATACA ATAACCTAGA TATCACGGTG ACCCAGGCCC
1561TTCAGCACCG TTCACAGTAT TTTGAAGGTG TTGTGAAGTG CAATAAGCTG GAAATACTGG
1621AGACCATCGT GGACAGAATA GTAAGAGCTG AGGTCCATCG GCTGGTGGTG GTAAATGAAG
1681CAGATAGTAT TGTGGGTATT ATTTCCCTGT CGGACATTCT GCAAGCCCTG ATCCTCACAC
1741CAGCAGGTGC CAAACAAAAG GAGACAGAAA CGGAGTAGAA CCCAGCTTTC TTGTACAAAG
1801TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1861GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1921ACGAGGCGAA TAATCCAGTC CTGCTCTCAC GCGTTAAGTC gacaatcaac ctctggatta
1981caaaatttgt gaaagatt

Download FASTA (ORF) (Full)