Construct: ORF TRCN0000489119

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020452.1_s317c1
DNA Barcode:
TACCTGTAGTGACCTCCCATAAGG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
TLK2 (11011)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489119
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11011 TLK2 tousled like kinase 2 NM_006852.4 100% 100%
2 human 11011 TLK2 tousled like kinase 2 XM_017024044.1 100% 100%
3 human 11011 TLK2 tousled like kinase 2 XM_017024046.1 100% 100%
4 human 11011 TLK2 tousled like kinase 2 XM_017024047.1 100% 100%
5 human 11011 TLK2 tousled like kinase 2 NM_001284333.2 97.1% 97.1% 1122_1187del
6 human 11011 TLK2 tousled like kinase 2 XM_011524216.2 97.1% 97.1% 1122_1187del
7 human 11011 TLK2 tousled like kinase 2 XM_011524217.3 97.1% 97.1% 1122_1187del
8 human 11011 TLK2 tousled like kinase 2 XM_011524222.3 97.1% 97.1% 1122_1187del
9 human 11011 TLK2 tousled like kinase 2 XM_024450553.1 97.1% 97.1% 1122_1187del
10 human 11011 TLK2 tousled like kinase 2 XM_024450552.1 96.2% 96.2% 1_21del;1143_1208del
11 human 11011 TLK2 tousled like kinase 2 XM_011524218.2 95.8% 95.8% 627_628ins93
12 human 11011 TLK2 tousled like kinase 2 NM_001284363.1 95.7% 95.7% 266_267ins96
13 human 11011 TLK2 tousled like kinase 2 XM_017024051.2 95.7% 95.7% 266_267ins96
14 human 11011 TLK2 tousled like kinase 2 XM_017024052.2 95.7% 95.7% 266_267ins96
15 human 11011 TLK2 tousled like kinase 2 XM_017024053.2 95.7% 95.7% 266_267ins96
16 human 11011 TLK2 tousled like kinase 2 XM_011524215.2 93.1% 93.1% 627_628ins93;1029_1094del
17 human 11011 TLK2 tousled like kinase 2 XM_011524221.2 93% 93% 266_267ins96;1026_1091del
18 human 11011 TLK2 tousled like kinase 2 XM_017024048.2 93% 93% 266_267ins96;1026_1091del
19 human 11011 TLK2 tousled like kinase 2 XM_017024049.2 93% 93% 266_267ins96;1026_1091del
20 human 11011 TLK2 tousled like kinase 2 XM_017024054.2 91.6% 91.6% 530_531ins189
21 human 11011 TLK2 tousled like kinase 2 XM_017024045.1 90.2% 90.2% 1_138del;404_405ins96
22 human 11011 TLK2 tousled like kinase 2 XM_011524220.2 88.9% 88.9% 530_531ins189;933_998del
23 human 11011 TLK2 tousled like kinase 2 XM_011524223.3 88.9% 88.9% 266_267ins96;531_532ins93;933_998del
24 human 11011 TLK2 tousled like kinase 2 XM_017024055.2 87.3% 87.3% 266_267ins96;434_435ins189
25 human 11011 TLK2 tousled like kinase 2 XM_017024050.1 86.3% 86.3% 1_138del;404_405ins96;669_670ins93
26 human 11011 TLK2 tousled like kinase 2 XM_017024056.2 86.1% 84% 0_1ins172;50_51ins140
27 human 11011 TLK2 tousled like kinase 2 XM_017024057.1 86.1% 84% 0_1ins172;50_51ins140
28 human 11011 TLK2 tousled like kinase 2 XM_011524224.3 83.6% 81.6% 0_1ins172;50_51ins140;810_875del
29 human 11011 TLK2 tousled like kinase 2 XM_011524225.3 83.6% 81.6% 0_1ins172;50_51ins140;810_875del
30 human 11011 TLK2 tousled like kinase 2 XM_011524226.2 83.6% 81.6% 0_1ins172;50_51ins140;810_875del
31 human 11011 TLK2 tousled like kinase 2 XM_011524227.2 83.6% 81.6% 0_1ins172;50_51ins140;810_875del
32 human 11011 TLK2 tousled like kinase 2 XM_011524228.3 83.6% 81.6% 0_1ins172;50_51ins140;810_875del
33 human 11011 TLK2 tousled like kinase 2 NM_001330418.2 80.1% 80.1% 0_1ins447
34 human 11011 TLK2 tousled like kinase 2 XM_017024059.1 80.1% 80.1% 0_1ins447
35 human 11011 TLK2 tousled like kinase 2 XM_011524229.2 77.8% 77.8% 0_1ins447;675_740del
36 human 11011 TLK2 tousled like kinase 2 XM_017024060.1 71.7% 71.7% 0_1ins447;83_84ins189
37 human 11011 TLK2 tousled like kinase 2 XM_011524231.1 63.9% 63.2% (many diffs)
38 human 101928451 LOC101928451 uncharacterized LOC101928451 XR_927955.2 42.3% (many diffs)
39 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001112705.2 93.6% 99.3% (many diffs)
40 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533312.3 93.6% 99.3% (many diffs)
41 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533313.3 93.6% 99.3% (many diffs)
42 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_017314550.1 91.1% 96.7% (many diffs)
43 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001294331.1 89.3% 95.2% (many diffs)
44 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_011249014.2 89.3% 95.2% (many diffs)
45 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533314.3 87.4% 92.5% (many diffs)
46 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533315.3 87% 92.7% (many diffs)
47 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533316.3 86.5% 91.9% (many diffs)
48 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_017314552.1 85.5% 90.9% (many diffs)
49 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001294334.1 85.1% 86.2% (many diffs)
50 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_017314551.1 83.4% 88.5% (many diffs)
51 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001294329.1 80.4% 83.7% (many diffs)
52 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XM_006533320.3 80.4% 83.7% (many diffs)
53 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... NM_001294330.1 77% 77.9% (many diffs)
54 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XR_001780005.1 27.6% (many diffs)
55 mouse 24086 Tlk2 tousled-like kinase 2 (Arab... XR_001780006.1 27.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2322
ORF length:
2250
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgatggaa gaattgcata gcctggaccc acgacggcag gaattattgg
121aggccaggtt tactggagta ggtgttagta agggaccact taatagtgag tcttccaacc
181agagcttgtg cagcgtcgga tccttgagtg ataaagaagt agagactccc gagaaaaagc
241agaatgacca gcgaaatcgg aaaagaaaag ctgaaccata tgaaactagc caagggaaag
301gcactcctag gggacataaa attagtgatt actttgagtt tgctggggga agcgcgccag
361gaaccagccc tggcagaagt gttccaccag ttgcacgatc ctcaccgcaa cattccttat
421ccaatccctt accgcgacga gtagaacagc ccctctatgg tttagatggc agtgctgcaa
481aggaggcaac ggaggagcag tctgctctgc caaccctcat gtcagtgatg ctagcaaaac
541ctcggcttga cacagagcag ctggcgcaaa ggggagctgg cctctgcttc acttttgttt
601cagctcagca aaacagtccc tcatctacgg gatctggcaa cacagagcat tcctgcagct
661cccaaaaaca gatctccatc cagcacagac agacccagtc cgacctcaca atagaaaaaa
721tatctgcact agaaaacagt aagaattctg acttagagaa gaaggaggga agaatagatg
781atttattaag agccaactgt gatttgagac ggcagattga tgaacagcaa aagatgctag
841agaaatacaa ggaacgatta aatagatgtg tgacaatgag caagaaactc cttatagaaa
901agtcaaaaca agagaagatg gcgtgtagag ataagagcat gcaagaccgc ttgagactgg
961gccactttac tactgtccga cacggagcct catttactga acagtggaca gatggttatg
1021cttttcagaa tcttatcaag caacaggaaa ggataaattc acagagggaa gagatagaaa
1081gacaacggaa aatgttagca aagcggaaac ctcctgccat gggtcaggcc cctcctgcaa
1141ccaatgagca gaaacagcgg aaaagcaaga ccaatggagc tgaaaatgaa acgttaacgt
1201tagcagaata ccatgaacaa gaagaaatct tcaaactcag attaggtcat cttaaaaagg
1261aggaagcaga gatccaggca gagctggaga gactagaaag ggttagaaat ctacatatca
1321gggaactaaa aaggatacat aatgaagata attcacaatt taaagatcat ccaacgctaa
1381atgacagata tttgttgtta catcttttgg gtagaggagg tttcagtgaa gtttacaagg
1441catttgatct aacagagcaa agatacgtag ctgtgaaaat tcaccagtta aataaaaact
1501ggagagatga gaaaaaggag aattaccaca agcatgcatg tagggaatac cggattcata
1561aagagctgga tcatcccaga atagttaagc tgtatgatta cttttcactg gatactgact
1621cgttttgtac agtattagaa tactgtgagg gaaatgatct ggacttctac ctgaaacagc
1681acaaattaat gtcggagaaa gaggcccggt ccattatcat gcagattgtg aatgctttaa
1741agtacttaaa tgaaataaaa cctcccatca tacactatga cctcaaacca ggtaatattc
1801ttttagtaaa tggtacagcg tgtggagaga taaaaattac agattttggt ctttcgaaga
1861tcatggatga tgatagctac aattcagtgg atggcatgga gctaacatca caaggtgctg
1921gtacttattg gtatttacca ccagagtgtt ttgtggttgg gaaagaacca ccaaagatct
1981caaataaagt tgatgtgtgg tcggtgggtg tgatcttcta tcagtgtctt tatGGAAGGA
2041AGCCTTTTGG CCATAACCAG TCTCAGCAAG ACATCCTACA AGAGAATACG ATTCTTAAAG
2101CTACTGAAGT GCAGTTCCCG CCAAAGCCAG TAGTAACACC TGAAGCAAAG GCGTTTATTC
2161GACGATGCTT GGCCTACCGA AAGGAGGACC GCATTGATGT CCAGCAGCTG GCCTGTGATC
2221CCTACTTGTT GCCTCACATC CGAAAGTCAG TCTCTACAAG TAGCCCTGCT GGAGCTGCTA
2281TTGCATCAAC CTCTGGGGCG TCCAATAACA GTTCTTCTAA TTAGGACCCA GCTTTCTTGT
2341ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
2401AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
2461GAAAGGACGA TACCTGTAGT GACCTCCCAT AAGGACGCGT TAAGTCgaca atcaacctct
2521ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)