Construct: ORF TRCN0000489242
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020633.1_s317c1
- DNA Barcode:
- TTAAAATAGGCACATTTAAGAAGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP4K3 (8491)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489242
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8491 | MAP4K3 | mitogen-activated protein k... | NM_003618.4 | 99.9% | 100% | 189T>C |
| 2 | human | 8491 | MAP4K3 | mitogen-activated protein k... | NM_001270425.2 | 97.6% | 97.6% | 189T>C;1056_1057ins63 |
| 3 | human | 8491 | MAP4K3 | mitogen-activated protein k... | XR_939730.2 | 60.2% | (many diffs) | |
| 4 | human | 8491 | MAP4K3 | mitogen-activated protein k... | XM_024453183.1 | 50% | 50% | 0_1ins1341 |
| 5 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | NM_001290345.1 | 88.9% | 96% | (many diffs) |
| 6 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524192.2 | 87.8% | 94.9% | (many diffs) |
| 7 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524196.2 | 87.2% | 94.1% | (many diffs) |
| 8 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524197.2 | 86.6% | 93.8% | (many diffs) |
| 9 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524194.2 | 86.2% | 93% | (many diffs) |
| 10 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524195.2 | 85.5% | 92.7% | (many diffs) |
| 11 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524198.2 | 84.9% | 91.9% | (many diffs) |
| 12 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524199.3 | 78.5% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2754
- ORF length:
- 2682
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaacccc ggcttcgatt tgtcccgccg gaacccgcag gaggacttcg 121 agctgattca gcgcatcggc agcggcacct acggcgacgt ctacaaggca cggaatgtta 181 acactggtga attagcagca attaaagtaa taaaattgga accaggagaa gactttgcag 241 ttgtgcagca agaaattatc atgatgaaag actgtaaaca cccaaatatt gttgcttatt 301 ttggaagcta tctcaggcga gataagcttt ggatttgcat ggagttttgt ggaggtggtt 361 ctttacagga tatttatcac gtaactggac ctctgtcaga actgcaaatt gcatatgtta 421 gcagagaaac actgcaggga ttatattatc ttcacagtaa aggaaaaatg cacagagata 481 taaagggagc taacattcta ttaacggata atggtcatgt gaaattggct gattttggag 541 tatctgcaca gataacagct acaattgcca aacggaagtc tttcattggc acaccatatt 601 ggatggctcc agaagttgca gctgttgaga ggaagggggg ttacaatcaa ctctgtgatc 661 tctgggcagt gggaatcact gccatagaac ttgcagagct tcagcctcct atgtttgact 721 tacacccaat gagagcatta tttctaatga caaaaagcaa ttttcagcct cctaaactaa 781 aggataaaat gaaatggtca aatagttttc atcactttgt gaaaatggca cttaccaaaa 841 atccgaaaaa aagacctact gctgaaaaat tattacagca tccttttgta acacaacatt 901 tgacacggtc tttggcaatc gagctgttgg ataaagtaaa taatccagat cattccactt 961 accatgattt cgatgatgat gatcctgagc ctcttgttgc tgtaccacat agaattcact 1021 caacaagtag aaacgtgaga gaagaaaaaa cacgctcaga gataaccttt ggccaagtga 1081 aatttgatcc acccttaaga aaggagacag aaccacatca tgaacttccc gacagtgatg 1141 gttttttgga cagttcagaa gaaatatact acactgcaag atctaatctg gatctgcaac 1201 tggaatatgg acaaggacac caaggtggtt actttttagg tgcaaacaag agtcttctca 1261 agtctgttga agaagaattg catcagcgag gacacgtcgc acatttagaa gatgatgaag 1321 gagatgatga tgaatctaaa cactcaactc tgaaagcaaa aattccacct cctttgccac 1381 caaagcctaa gtctatcttc ataccacagg aaatgcattc tactgaggat gaaaatcaag 1441 gaacaatcaa gagatgtccc atgtcaggga gcccagcaaa gccatcccaa gttccaccta 1501 gaccaccacc tcccagatta cccccacaca aacctgttgc cttaggaaat ggaatgagct 1561 ccttccagtt aaatggtgaa cgagatggct cattatgtca acaacagaat gaacatagag 1621 gcacaaacct ttcaagaaaa gaaaagaaag atgtaccaaa gcctattagt aatggtcttc 1681 ctccaacacc taaagtgcat atgggtgcat gtttttcaaa agtttttaat gggtgtccct 1741 tgaaaattca ctgtgcatca tcatggataa acccagatac aagagatcag tacttgatat 1801 ttggtgccga agaagggatt tataccctca atcttaatga acttcatgaa acatcaatgg 1861 aacagctatt ccctcgaagg tgtacatggt tgtatgtaat gaacaattgc ttgctatcaa 1921 tatctggtaa agcttctcag ctttattccc ataatttacc agggcttttt gattatgcaa 1981 gacaaatgca aaagttacct gttgctattc cagcacacaa actccctgac agaatactgc 2041 caaggaaatt ttctgtatca gcaaaaatcc ctgaaaccaa atggtgccag aagtgttgtg 2101 ttgtaagaaa tccttacacg ggccataaat acctatgtgg agcacttcag actagcattg 2161 ttctattaga atgggttgaa ccaatgcaga aatttatgtt aattaagcac atagattttc 2221 ctataccatg tccacttaga atgtttgaaa tgctggtagt tcctgaacag gagtaccctt 2281 tagtttgtgt tggtgtcagt agaggtagag acttcaacca agtggttcga tttgagacgg 2341 tcaatccaaa ttctacctct tcatggttta cagaatcaga taCCCCACAG ACAAATGTTA 2401 CTCATGTAAC CCAACTGGAG AGAGATACCA TCCTTGTATG CTTGGACTGT TGTATAAAAA 2461 TAGTAAATCT CCAAGGAAGA TTAAAATCTA GCAGGAAATT GTCATCAGAA CTCACCTTTG 2521 ATTTCCAGAT TGAATCAATA GTGTGCCTAC AAGACAGTGT GCTAGCTTTC TGGAAACATG 2581 GAATGCAAGG TAGAAGTTTT AGATCTAATG AGGTAACACA AGAAATTTCA GATAGCACAA 2641 GAATTTTCAG GCTGCTTGGA TCTGACAGGG TCGTGGTTTT GGAAAGTAGG CCAACTGATA 2701 ACCCCACAGC AAATAGCAAT TTGTACATCC TGGCGGGTCA TGAAAACAGT TACTGAGACC 2761 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2821 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2881 ATATATCTTG TGGAAAGGAC GATTAAAATA GGCACATTTA AGAAGGACGC GTTAAGTCga 2941 caatcaacct ctggattaca aaatttgtga aagatt