Construct: ORF TRCN0000489380
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021700.1_s317c1
- DNA Barcode:
- AGAGTAATAAGTTCTAAGTCATCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SP1 (6667)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489380
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6667 | SP1 | Sp1 transcription factor | NM_138473.3 | 100% | 100% | |
| 2 | human | 6667 | SP1 | Sp1 transcription factor | NM_003109.1 | 99.1% | 99.1% | 0_1ins21 |
| 3 | human | 6667 | SP1 | Sp1 transcription factor | NM_001251825.2 | 93.8% | 93.8% | 159_160ins144 |
| 4 | mouse | 20683 | Sp1 | trans-acting transcription ... | NM_013672.2 | 91.5% | 94.7% | (many diffs) |
| 5 | mouse | 20683 | Sp1 | trans-acting transcription ... | XM_017316523.1 | 90.6% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2424
- ORF length:
- 2355
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gagcgaccaa gatcactcca tggatgaaat gacagctgtg gtgaaaattg 121 aaaaaggagt tggtggcaat aatgggggca atggtaatgg tggtggtgcc ttttcacagg 181 ctcgaagtag cagcacaggc agtagcagca gcactggagg aggagggcag gagtcccagc 241 catccccttt ggctctgctg gcagcaactt gcagcagaat tgagtcaccc aatgagaaca 301 gcaacaactc ccagggcccg agtcagtcag ggggaacagg tgagcttgac ctcacagcca 361 cacaactttc acagggtgcc aatggctggc agatcatctc ttcctcctct ggggctaccc 421 ctacctcaaa ggaacagagt ggcagcagta ccaatggcag caatggcagt gagtcttcca 481 agaatcgcac agtctctggt gggcagtatg ttgtggctgc cgctcccaac ttacagaacc 541 agcaagttct gacaggacta cctggagtga tgcctaatat tcagtatcaa gtaatcccac 601 agttccagac cgttgatggg caacagctgc agtttgctgc cactggggcc caagtgcagc 661 aggatggttc tggtcaaata cagatcatac caggtgcaaa ccaacagatt atcacaaatc 721 gaggaagtgg aggcaacatc attgctgcta tgccaaacct actccagcag gctgtccccc 781 tccaaggcct ggctaataat gtactctcag gacagactca gtatgtgacc aatgtaccag 841 tggccctgaa tgggaacatc accttgctac ctgtcaacag cgtttctgca gctaccttga 901 ctcccagctc tcaggcagtc acgatcagca gctctgggtc ccaggagagt ggctcacagc 961 ctgtcacctc agggactacc atcagttctg ccagcttggt atcatcacaa gccagttcca 1021 gctccttttt caccaatgcc aatagctact caactactac taccaccagc aacatgggaa 1081 ttatgaactt tactaccagt ggatcatcag ggaccaactc tcaaggccag acaccccaga 1141 gggtcagtgg gctacagggg tctgatgctc tgaacatcca gcaaaaccag acatctggag 1201 gctcattgca agcaggccag caaaaagaag gagagcaaaa ccagcagaca cagcagcaac 1261 aaattcttat ccagcctcag ctagttcaag ggggacaggc cctccaggcc ctccaagcag 1321 caccattgtc agggcagacc tttacaactc aagccatctc ccaggaaacc ctccagaacc 1381 tccagcttca ggctgttcca aactctggtc ccatcatcat ccggacacca acagtggggc 1441 ccaatggaca ggtcagttgg cagactctac agctgcagaa cctccaagtt cagaacccac 1501 aagcccaaac aatcacctta gccccaatgc agggtgtttc cttggggcag accagcagca 1561 gcaacaccac tctcacaccc attgcctcag ctgcttccat tcctgctggc acagtcactg 1621 tgaatgctgc tcaactctcc tccatgccag gcctccagac cattaacctc agtgcattgg 1681 gtacttcagg aatccaggtg cacccaattc aaggcctgcc gttggctata gcaaatgccc 1741 caggtgatca tggagctcag cttggtctcc atggggctgg tggtgatgga atacatgatg 1801 acacagcagg tggagaggaa ggagaaaaca gcccagatgc ccaaccccaa gccggtcgga 1861 ggacccggcg ggaagcatgc acctgcccct actgtaaaga cagtgaagga aggggctcgg 1921 gggatcctgg caaaaagaaa cagcatattt gccacatcca aggctgtggg aaagtgtatg 1981 gcaagacctc tcacctgcgg gcacacttgc gctggcatac aggcgagagg ccatttatgt 2041 gtacctggtc atactgtggg aaacgcttca cacgttcgga tgagctacag aggcacaaac 2101 gtacacacac aggtgagaag aaatttgcct gccctgagtg tcctaagcgc ttcatgagga 2161 gtgaccacct gtcaaaacat atcaagaccc accagaataa gaagggaggc ccaggtgtag 2221 ctctgagtgt gggCACTTTG CCCCTGGACA GTGGGGCAGG TTCAGAAGGC AGTGGCACTG 2281 CCACTCCTTC AGCCCTTATT ACCACCAATA TGGTAGCCAT GGAGGCCATC TGTCCAGAGG 2341 GCATTGCCCG TCTTGCCAAC AGTGGCATCA ACGTCATGCA GGTGGCAGAT CTGCAGTCCA 2401 TTAATATCAG TGGCAATGGC TTCTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 2461 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2521 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGAGTAAT 2581 AAGTTCTAAG TCATCTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2641 aagatt