Construct: ORF TRCN0000489411
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019689.1_s317c1
- DNA Barcode:
- AAGTGGCATAGCCCCCTCAAGCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMK2B (816)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489411
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_001293170.2 | 99.9% | 99.8% | 1626_1627insG |
2 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172078.3 | 99.9% | 99.8% | 1626_1627insG |
3 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_005249862.3 | 97.1% | 97% | 1130_1131ins45;1581_1582insG |
4 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_006715784.2 | 95.5% | 95.3% | 1058_1059ins72;1554_1555insG |
5 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172079.2 | 95.4% | 95.2% | 947_947delCins73;1554_1555insG |
6 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172080.3 | 95.3% | 95.2% | 944_945ins75;1551_1552insG |
7 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172081.3 | 92.6% | 92.4% | 947_947delCins73;1058_1059ins45;1509_1510insG |
8 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012664.1 | 92.5% | 92.4% | 944_945ins75;1055_1056ins45;1506_1507insG |
9 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_005249864.3 | 91% | 90.7% | 947_947delCins73;986_987ins72;1482_1483insG |
10 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012665.1 | 90.9% | 90.7% | 944_945ins75;983_984ins72;1479_1480insG |
11 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172082.3 | 90.7% | 90.6% | 1058_1059ins72;1229_1230ins78;1476_1477insG |
12 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172083.3 | 88.2% | 88% | 947_947delCins73;984_985ins117;1437_1438insG |
13 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_024446945.1 | 86.2% | 86.1% | 1226_1483del;1884_1885insG |
14 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172084.3 | 82.7% | 82.6% | 944_945ins279;1347_1348insG |
15 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012662.1 | 82.2% | 82.1% | 944_945ins75;1151_1408del;1809_1810insG |
16 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012661.1 | 81.6% | 78.4% | (many diffs) |
17 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_001220.5 | 81.3% | 81.2% | 1226_1597del;1998_1999insG |
18 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515555.1 | 81.3% | 81.2% | 1224_1595del;1998_1999insG |
19 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515553.1 | 80.7% | 80.6% | 1224_1610del;2013_2014insG |
20 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515558.2 | 79.9% | 67.9% | (many diffs) |
21 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001204492.1 | 78.5% | 85.2% | (many diffs) |
22 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_006715781.2 | 78.4% | 75.2% | (many diffs) |
23 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_172169.3 | 77.4% | 84.1% | (many diffs) |
24 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515549.1 | 77.2% | 74.2% | (many diffs) |
25 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367543.1 | 77.2% | 83.2% | (many diffs) |
26 | human | 818 | CAMK2G | calcium/calmodulin dependen... | XM_006717998.1 | 77.1% | 83.2% | (many diffs) |
27 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515547.1 | 76.4% | 76.3% | 1224_1724del;2127_2128insG |
28 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367517.1 | 76.2% | 83.6% | (many diffs) |
29 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_172170.5 | 76.2% | 83.6% | (many diffs) |
30 | human | 818 | CAMK2G | calcium/calmodulin dependen... | XM_024448218.1 | 76.2% | 82.7% | (many diffs) |
31 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012666.2 | 76.1% | 76% | 0_1ins387;1239_1240insG |
32 | human | 818 | CAMK2G | calcium/calmodulin dependen... | XM_005270205.1 | 76% | 84% | (many diffs) |
33 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367531.1 | 75.8% | 81.6% | (many diffs) |
34 | human | 818 | CAMK2G | calcium/calmodulin dependen... | XM_017016730.1 | 75.7% | 81.6% | (many diffs) |
35 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367530.1 | 75.1% | 82.5% | (many diffs) |
36 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_172173.3 | 75% | 83.6% | (many diffs) |
37 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367532.1 | 75% | 82.1% | (many diffs) |
38 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367516.1 | 74.7% | 78.8% | (many diffs) |
39 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367527.1 | 74.1% | 78.6% | (many diffs) |
40 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001222.4 | 73.7% | 82.3% | (many diffs) |
41 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012660.1 | 73.7% | 70.7% | (many diffs) |
42 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367544.1 | 73.6% | 79.8% | (many diffs) |
43 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515554.1 | 73.6% | 70.7% | (many diffs) |
44 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367545.1 | 73.4% | 77.1% | (many diffs) |
45 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012667.2 | 73.3% | 73.2% | 0_1ins387;743_744ins45;1194_1195insG |
46 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515551.1 | 73% | 72.9% | 1058_1059ins72;1152_1652del;2055_2056insG |
47 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515550.1 | 72.9% | 72.8% | 947_947delCins73;1152_1652del;2055_2056insG |
48 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515552.1 | 72.8% | 72.8% | 944_945ins75;1149_1649del;2052_2053insG |
49 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367546.1 | 72.5% | 78.8% | (many diffs) |
50 | human | 818 | CAMK2G | calcium/calmodulin dependen... | XM_005270198.1 | 72.5% | 79.8% | (many diffs) |
51 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367534.1 | 72.4% | 78% | (many diffs) |
52 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367548.1 | 72.3% | 78% | (many diffs) |
53 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012669.2 | 71.7% | 71.6% | 0_1ins387;671_672ins72;1167_1168insG |
54 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012668.2 | 71.6% | 71.4% | 0_1ins387;560_560delCins73;1167_1168insG |
55 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_024446946.1 | 71.5% | 71.4% | 0_1ins387;557_558ins75;1164_1165insG |
56 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_172171.2 | 71.5% | 78.3% | (many diffs) |
57 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367547.1 | 71.3% | 76.9% | (many diffs) |
58 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_001369025.2 | 71.2% | 75.3% | (many diffs) |
59 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_001363990.1 | 71.1% | 75.3% | (many diffs) |
60 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_171825.2 | 71.1% | 75.3% | (many diffs) |
61 | human | 818 | CAMK2G | calcium/calmodulin dependen... | XM_006717996.1 | 71.1% | 76.5% | (many diffs) |
62 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367518.1 | 70.4% | 77.3% | (many diffs) |
63 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367533.1 | 70.3% | 78.3% | (many diffs) |
64 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367525.1 | 70.3% | 76.9% | (many diffs) |
65 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515556.1 | 69.5% | 69.4% | (many diffs) |
66 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515557.1 | 69.5% | 69.4% | (many diffs) |
67 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012670.2 | 68.8% | 68.6% | (many diffs) |
68 | human | 818 | CAMK2G | calcium/calmodulin dependen... | XM_024448219.1 | 64.3% | 69.4% | (many diffs) |
69 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367539.1 | 60.3% | 65.1% | (many diffs) |
70 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515559.2 | 58.2% | 58.1% | 0_1ins387;837_1337del;1740_1741insG |
71 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012663.2 | 54.8% | 54.7% | (many diffs) |
72 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | NM_007595.5 | 92.5% | 99% | (many diffs) |
73 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314239.1 | 92.3% | 98.8% | (many diffs) |
74 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514475.1 | 89.8% | 96.3% | (many diffs) |
75 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314240.1 | 89.6% | 96.1% | (many diffs) |
76 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514476.1 | 88% | 94.4% | (many diffs) |
77 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514477.1 | 87.9% | 94.4% | (many diffs) |
78 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | NM_001174054.1 | 86.3% | 92.5% | (many diffs) |
79 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514479.2 | 85.3% | 91.7% | (many diffs) |
80 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | NM_001174053.1 | 78.9% | 84.9% | (many diffs) |
81 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314237.1 | 76% | 78.2% | (many diffs) |
82 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514465.2 | 75.3% | 80.6% | (many diffs) |
83 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514464.2 | 75.2% | 80.5% | (many diffs) |
84 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | NM_001039138.2 | 74.9% | 83.6% | (many diffs) |
85 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514469.2 | 72.4% | 74.6% | (many diffs) |
86 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | NM_001039139.2 | 72.4% | 82.3% | (many diffs) |
87 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514470.3 | 72.3% | 74.6% | (many diffs) |
88 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514467.2 | 71.6% | 76.9% | (many diffs) |
89 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314238.1 | 71.5% | 76.9% | (many diffs) |
90 | mouse | 12322 | Camk2a | calcium/calmodulin-dependen... | XM_017317805.2 | 70.8% | 75.3% | (many diffs) |
91 | mouse | 12322 | Camk2a | calcium/calmodulin-dependen... | NM_177407.4 | 70.6% | 75.3% | (many diffs) |
92 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | XM_006518492.2 | 70.4% | 78.3% | (many diffs) |
93 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | XM_006518490.3 | 69.9% | 76.5% | (many diffs) |
94 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | XM_006518495.2 | 69.3% | 77.3% | (many diffs) |
95 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314241.1 | 51.8% | 51.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1704
- ORF length:
- 1629
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggcc accacggtga cctgcacccg cttcaccgac gagtaccagc 121 tctacgagga tattggcaag ggggctttct ctgtggtccg acgctgtgtc aagctctgca 181 ccggccatga gtatgcagcc aagatcatca acaccaagaa gctgtcagcc agagatcacc 241 agaagctgga gagagaggct cggatctgcc gccttctgaa gcattccaac atcgtgcgtc 301 tccacgacag catctccgag gagggcttcc actacctggt cttcgatctg gtcactggtg 361 gggagctctt tgaagacatt gtggcgagag agtactacag cgaggctgat gccagtcact 421 gtatccagca gatcctggag gccgttctcc attgtcacca aatgggggtc gtccacagag 481 acctcaagcc ggagaacctg cttctggcca gcaagtgcaa aggggctgca gtgaagctgg 541 cagacttcgg cctagctatc gaggtgcagg gggaccagca ggcatggttt ggtttcgctg 601 gcacaccagg ctacctgtcc cctgaggtcc ttcgcaaaga ggcgtatggc aagcctgtgg 661 acatctgggc atgtggggtg atcctgtaca tcctgctcgt gggctaccca cccttctggg 721 acgaggacca gcacaagctg taccagcaga tcaaggctgg tgcctatgac ttcccgtccc 781 ctgagtggga caccgtcact cctgaagcca aaaacctcat caaccagatg ctgaccatca 841 accctgccaa gcgcatcaca gcccatgagg ccctgaagca cccgtgggtc tgccaacgct 901 ccacggtagc atccatgatg cacagacagg agactgtgga gtgtctgaaa aagttcaatg 961 ccaggagaaa gctcaaggga gccatcctca ccaccatgct ggccacacgg aatttctcag 1021 tgggcagaca gaccaccgct ccggccacaa tgtccaccgc ggcctccggc accaccatgg 1081 ggctggtgga acaagccaag agtttactca acaagaaagc agatggagtc aagccccaga 1141 cgaatagcac caaaaacagt gcagccgcca ccagccccaa agggacgctt cctcctgccg 1201 ccctggagcc tcaaaccacc gtcatccata acccagtgga cgggattaag gagtcttctg 1261 acagtgccaa taccaccata gaggatgaag acgctaaagc ccggaagcag gagatcatta 1321 agaccacgga gcagctcatc gaggccgtca acaacggtga ctttgaggcc TACGCGAAAA 1381 TCTGTGACCC AGGGCTGACC TCGTTTGAGC CTGAAGCACT GGGCAACCTG GTTGAAGGGA 1441 TGGACTTCCA CAGATTCTAC TTCGAGAACC TGCTGGCCAA GAACAGCAAG CCGATCCACA 1501 CGACCATCCT GAACCCACAC GTGCACGTCA TTGGAGAGGA TGCCGCCTGC ATCGCTTACA 1561 TCCGGCTCAC GCAGTACATT GACGGGCAGG GCCGGCCCCG CACCAGCCAG TCTGAGGAGA 1621 CCCGCGTGTG GCACCGCCGC GACGGCAAGT GGCAGAACGT GCACTTCCAC TGCTCGGGCG 1681 CGCCTGTGGC CCCGCTGCAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1741 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1801 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAAGT GGCATAGCCC 1861 CCTCAAGCTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt