Construct: ORF TRCN0000489560
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020101.1_s317c1
- DNA Barcode:
- TTCAAGTCGTGCGGGCTTGCCTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHA6 (285220)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489560
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285220 | EPHA6 | EPH receptor A6 | NM_173655.4 | 100% | 100% | |
| 2 | human | 285220 | EPHA6 | EPH receptor A6 | NM_001278300.2 | 83.9% | 83.9% | 961_1152del |
| 3 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006223.1 | 82.4% | 81.4% | (many diffs) |
| 4 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006220.1 | 62.4% | 61.6% | (many diffs) |
| 5 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006217.1 | 57.8% | 54.6% | (many diffs) |
| 6 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006218.1 | 57.8% | 54.6% | (many diffs) |
| 7 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006216.1 | 55.4% | 52.4% | (many diffs) |
| 8 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006215.1 | 53.9% | 51% | (many diffs) |
| 9 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006214.1 | 41% | 38.6% | (many diffs) |
| 10 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006213.1 | 32.4% | 30.6% | (many diffs) |
| 11 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006212.1 | 31.7% | 29.9% | (many diffs) |
| 12 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006211.1 | 31.5% | 29.7% | (many diffs) |
| 13 | human | 285220 | EPHA6 | EPH receptor A6 | NM_001080448.3 | 28.4% | 26.8% | (many diffs) |
| 14 | human | 285220 | EPHA6 | EPH receptor A6 | XM_006713592.3 | 28% | 26.4% | (many diffs) |
| 15 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006210.1 | 24.4% | 22.6% | (many diffs) |
| 16 | human | 285220 | EPHA6 | EPH receptor A6 | XR_001740110.1 | 7.4% | (many diffs) | |
| 17 | mouse | 13840 | Epha6 | Eph receptor A6 | XM_006521787.2 | 29.2% | 30.5% | (many diffs) |
| 18 | mouse | 13840 | Epha6 | Eph receptor A6 | NM_007938.2 | 25.2% | 26.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 1101
- ORF length:
- 1002
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcaccctcg agcaggagat gaaagactct ccatttcaag 121 tgacaaaact gtactggctt aatgaaaagt gggactttat tgcctcagct tctgacatgg 181 cagcagaaca aggacagatt ctcgtgatag ccaccgccgc tgttggcgga ttcactctcc 241 tcgtcatcct cactttattc ttcttgatca ctgggagatg tcagtggtac ataaaagcca 301 agatgaagtc agaagagaag agaagaaacc acttacagaa tgggcatttg cgcttcccgg 361 gaattaaaac ttacattgat ccagatacat atgaagaccc atccctagca gtccatgaat 421 ttgcaaagga gattgatccc tcaagaattc gtattgagag agtcattggg gcaggtgaat 481 ttggagaagt ctgtagtggg cgtttgaaga caccagggaa aagagagatc ccagttgcca 541 ttaaaacttt gaaaggtggc cacatggatc ggcaaagaag agattttcta agagaagcta 601 gtatcatggg ccagtttgac catccaaaca tcattcgcct agaaggggtt gtcaccaaaa 661 gatccttccc ggccattggg gtggaggcgt tttgccccag cttcctgagg gcagggtttt 721 taaatagcat ccaggccccg catccagtgc cagggggagg atctttgccc cccaggattc 781 cTGCTGGCAG ACCAGTAATG ATTGTGGTGG AATATATGGA GAATGGATCC CTAGACTCCT 841 TTTTGCGGAA GCATGATGGC CACTTCACAG TCATCCAGTT GGTCGGAATG CTCCGAGGCA 901 TTGCATCAGG CATGAAGTAT CTTTCTGATA TGGGTTATGT TCATCGAGAC CTAGCGGCTC 961 GGAATATACT GGTCAATAGC AACTTAGTAT GCAAAGTTTC TGATTTTGGT CTCTCCAGAG 1021 TGCTGGAAGA TGATCCAGAA GCTGCTTATA CAACAACTGA CCTCTTCCAA ACTCTAACAC 1081 TTAACCTCTG CTATTCTGCA AAGGGTGGGC GCGCCGACCC AGCTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 ATTCAAGTCG TGCGGGCTTG CCTCTACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt