Construct: ORF TRCN0000489641
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021115.1_s317c1
- DNA Barcode:
- TGAGCCTACTCTGCAACGTGAGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RXFP1 (59350)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489641
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 59350 | RXFP1 | relaxin family peptide rece... | NM_021634.4 | 100% | 100% | |
| 2 | human | 59350 | RXFP1 | relaxin family peptide rece... | XM_017008518.2 | 99.8% | 99.8% | 1043_1044insCAG |
| 3 | human | 59350 | RXFP1 | relaxin family peptide rece... | XM_011532176.2 | 96.8% | 96.8% | 754_755ins72 |
| 4 | human | 59350 | RXFP1 | relaxin family peptide rece... | NM_001253727.1 | 96.5% | 96.4% | 287_367del |
| 5 | human | 59350 | RXFP1 | relaxin family peptide rece... | XM_011532174.1 | 96.4% | 96.3% | 287_367del;1124_1125insCAG |
| 6 | human | 59350 | RXFP1 | relaxin family peptide rece... | NM_001253728.2 | 95.6% | 95.5% | 187_188ins99 |
| 7 | human | 59350 | RXFP1 | relaxin family peptide rece... | NM_001253729.2 | 93.6% | 93.5% | 898_899ins144 |
| 8 | human | 59350 | RXFP1 | relaxin family peptide rece... | XM_011532175.1 | 93.4% | 93.3% | 287_367del;835_836ins72 |
| 9 | human | 59350 | RXFP1 | relaxin family peptide rece... | XM_017008517.1 | 93.3% | 93.2% | 287_367del;835_836ins72;1052_1053insCAG |
| 10 | human | 59350 | RXFP1 | relaxin family peptide rece... | NM_001363776.1 | 89.2% | 89.2% | 0_1ins243 |
| 11 | human | 59350 | RXFP1 | relaxin family peptide rece... | XM_017008522.1 | 89.1% | 89.1% | 0_1ins243;800_801insCAG |
| 12 | human | 59350 | RXFP1 | relaxin family peptide rece... | XM_017008519.1 | 86.2% | 86% | 0_1ins243;44_124del |
| 13 | human | 59350 | RXFP1 | relaxin family peptide rece... | XM_017008520.1 | 86.2% | 86% | 0_1ins243;44_124del |
| 14 | human | 59350 | RXFP1 | relaxin family peptide rece... | NM_001253730.1 | 82.6% | 82.6% | 0_1ins390;653_654insCAG |
| 15 | human | 59350 | RXFP1 | relaxin family peptide rece... | NM_001253732.2 | 81.2% | 79.1% | (many diffs) |
| 16 | human | 59350 | RXFP1 | relaxin family peptide rece... | NM_001253733.1 | 78% | 75.9% | (many diffs) |
| 17 | human | 59350 | RXFP1 | relaxin family peptide rece... | XM_017008526.1 | 78% | 75.9% | (many diffs) |
| 18 | human | 59350 | RXFP1 | relaxin family peptide rece... | NR_045584.2 | 66.7% | (many diffs) | |
| 19 | human | 59350 | RXFP1 | relaxin family peptide rece... | NR_045581.2 | 61.2% | 1_95del;282_305del;2391_3710del | |
| 20 | human | 59350 | RXFP1 | relaxin family peptide rece... | NR_045580.2 | 60.7% | 1_95del;282_334del;2420_3739del | |
| 21 | human | 59350 | RXFP1 | relaxin family peptide rece... | NR_045583.2 | 56.5% | (many diffs) | |
| 22 | human | 59350 | RXFP1 | relaxin family peptide rece... | NR_045582.2 | 56.1% | (many diffs) | |
| 23 | human | 59350 | RXFP1 | relaxin family peptide rece... | NR_045579.2 | 49.7% | (many diffs) | |
| 24 | human | 59350 | RXFP1 | relaxin family peptide rece... | XM_017008523.2 | 49.6% | 49.4% | (many diffs) |
| 25 | human | 59350 | RXFP1 | relaxin family peptide rece... | XM_011532179.2 | 47.9% | 47.5% | (many diffs) |
| 26 | human | 59350 | RXFP1 | relaxin family peptide rece... | XM_017008524.2 | 46.5% | 46.2% | (many diffs) |
| 27 | human | 59350 | RXFP1 | relaxin family peptide rece... | XM_017008525.1 | 45.3% | 44.9% | (many diffs) |
| 28 | mouse | 381489 | Rxfp1 | relaxin/insulin-like family... | NM_212452.1 | 85.9% | 85% | (many diffs) |
| 29 | mouse | 381489 | Rxfp1 | relaxin/insulin-like family... | XM_006501629.2 | 85.7% | 84.9% | (many diffs) |
| 30 | mouse | 381489 | Rxfp1 | relaxin/insulin-like family... | XM_006501630.2 | 71.8% | 71.8% | (many diffs) |
| 31 | mouse | 381489 | Rxfp1 | relaxin/insulin-like family... | XR_375538.2 | 24.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2343
- ORF length:
- 2271
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgacatct ggttctgtct tcttctacat cttaattttt ggaaaatatt 121 tttctcatgg gggtggacag gatgtcaagt gctcccttgg ctatttcccc tgtgggaaca 181 tcacaaagtg cttgcctcag ctcctgcact gtaacggtgt ggacgactgc gggaatcagg 241 ccgatgagga caactgtgga gacaacaatg gatggtctct gcaatttgac aaatattttg 301 ccagttacta caaaatgact tcccaatatc cttttgaggc agaaacacct gaatgtttgg 361 tcggttctgt gccagtgcaa tgtctttgcc aaggtctgga gcttgactgt gatgaaacca 421 atttacgagc tgttccatcg gtttcttcaa atgtgactgc aatgtcactt cagtggaact 481 taataagaaa gcttcctcct gattgcttca agaattatca tgatcttcag aagctgtacc 541 tgcaaaacaa taagattaca tccatctcca tctatgcttt cagaggactg aatagcctta 601 ctaaactgta tctcagtcat aacagaataa ccttcctgaa gccgggtgtt tttgaagatc 661 ttcacagact agaatggctg ataattgaag ataatcacct cagtcgaatt tccccaccaa 721 cattttatgg actaaattct cttattctct tagtcctgat gaataacgtc ctcacccgtt 781 tacctgataa acctctctgt caacacatgc caagactaca ttggctggac cttgaaggca 841 accatatcca taatttaaga aatttgactt ttatttcctg cagtaattta actgttttag 901 tgatgaggaa aaacaaaatt aatcacttaa atgaaaatac ttttgcacct ctccagaaac 961 tggatgaatt ggatttagga agtaataaga ttgaaaatct tccaccgctt atattcaagg 1021 acctgaagga gctgtcacaa ttgaatcttt cctataatcc aatccagaaa attcaagcaa 1081 accaatttga ttatcttgtc aaactcaagt ctctcagcct agaagggatt gaaatttcaa 1141 atatccaaca aaggatgttt agacctctta tgaatctctc tcacatatat tttaagaaat 1201 tccagtactg tgggtatgca ccacatgttc gcagctgtaa accaaacact gatggaattt 1261 catctctaga gaatctcttg gcaagcatta ttcagagagt atttgtctgg gttgtatctg 1321 cagttacctg ctttggaaac atttttgtca tttgcatgcg accttatatc aggtctgaga 1381 acaagctgta tgccatgtca atcatttctc tctgctgtgc cgactgctta atgggaatat 1441 atttattcgt gatcggaggc tttgacctaa agtttcgtgg agaatacaat aagcatgcgc 1501 agctgtggat ggagagtact cattgtcagc ttgtaggatc tttggccatt ctgtccacag 1561 aagtatcagt tttactgtta acatttctga cattggaaaa atacatctgc attgtctatc 1621 cttttagatg tgtgagacct ggaaaatgca gaacaattac agttctgatt ctcatttgga 1681 ttactggttt tatagtggct ttcattccat tgagcaataa ggaatttttc aaaaactact 1741 atggcaccaa tggagtatgc ttccctcttc attcagaaga tacagaaagt attggagccc 1801 agatttattc agtggcaatt tttcttggta ttaatttggc cgcatttatc atcatagttt 1861 tttcctatgg aagcatgttt tatagtgttc atcaaagtgc cataaCAGCA ACTGAAATAC 1921 GGAATCAAGT TAAAAAAGAG ATGATCCTTG CCAAACGTTT TTTCTTTATA GTATTTACTG 1981 ATGCATTATG CTGGATACCC ATTTTTGTAG TGAAATTTCT TTCACTGCTT CAGGTAGAAA 2041 TACCAGGTAC CATAACCTCT TGGGTAGTGA TTTTTATTCT GCCCATTAAC AGTGCTTTGA 2101 ACCCAATTCT CTATACTCTG ACCACAAGAC CATTTAAAGA AATGATTCAT CGGTTTTGGT 2161 ATAACTACAG ACAAAGAAAA TCTATGGACA GCAAAGGTCA GAAAACATAT GCTCCATCAT 2221 TCATCTGGGT GGAAATGTGG CCACTGCAGG AGATGCCACC TGAGTTAATG AAGCCGGACC 2281 TTTTCACATA CCCCTGTGAA ATGTCACTGA TTTCTCAATC AACGAGACTC AATTCCTATT 2341 CATGAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2401 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2461 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATGAGCCTAC TCTGCAACGT GAGTCACGCG 2521 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt