Construct: ORF TRCN0000489763
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020059.1_s317c1
- DNA Barcode:
- GGTGAGATCGGGAATAACTTGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AAK1 (22848)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489763
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22848 | AAK1 | AP2 associated kinase 1 | NM_014911.4 | 99.9% | 99.8% | 1770G>A;2883_2884insG |
| 2 | human | 22848 | AAK1 | AP2 associated kinase 1 | NM_001371575.1 | 95.8% | 95.7% | (many diffs) |
| 3 | human | 22848 | AAK1 | AP2 associated kinase 1 | NM_001371577.1 | 69% | 69% | (many diffs) |
| 4 | mouse | 269774 | Aak1 | AP2 associated kinase 1 | XM_006506192.1 | 88.4% | 90.9% | (many diffs) |
| 5 | mouse | 269774 | Aak1 | AP2 associated kinase 1 | NM_001040106.2 | 88.3% | 90.8% | (many diffs) |
| 6 | mouse | 269774 | Aak1 | AP2 associated kinase 1 | XM_006506191.1 | 86.6% | 87.8% | (many diffs) |
| 7 | mouse | 269774 | Aak1 | AP2 associated kinase 1 | NM_177762.6 | 82.6% | 85.4% | (many diffs) |
| 8 | mouse | 269774 | Aak1 | AP2 associated kinase 1 | XM_017321597.1 | 82.5% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2958
- ORF length:
- 2886
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaagaag tttttcgact cccggcgaga gcagggcggc tctggcctgg 121 gctccggctc cagcggagga gggggcagca cctcgggcct gggcagtggc tacatcggaa 181 gagtcttcgg catcgggcga cagcaggtca cagtggacga ggtgttggcg gaaggtggat 241 ttgctattgt atttctggtg aggacaagca atgggatgaa atgtgccttg aaacgcatgt 301 ttgtcaacaa tgagcatgat ctccaggtgt gcaagagaga aatccagata atgagggatc 361 tttcagggca caagaatatt gtgggttaca ttgattctag tatcaacaac gtgagtagcg 421 gtgatgtatg ggaagtgctc attctgatgg acttttgtag aggtggccag gtggtaaacc 481 tgatgaacca gcgcctgcaa acaggcttta cagagaatga agtgctccag atattttgtg 541 atacctgtga agctgttgcc cgcctgcatc agtgcaaaac tcctattatc caccgggacc 601 tgaaggttga aaacatcctc ttgcatgacc gaggccacta tgtcctgtgt gactttggaa 661 gcgccaccaa caaattccag aatccacaaa ctgagggagt caatgcagta gaagatgaga 721 ttaagaaata cacaacgctg tcctatcgag caccagaaat ggtcaacctg tacagtggca 781 aaatcatcac tacgaaggca gacatttggg ctcttggatg tttgttgtat aaattatgct 841 acttcacttt gccatttggg gaaagtcagg tggcaatttg tgatggaaac ttcacaattc 901 ctgataattc tcgatattct caagacatgc actgcctaat taggtatatg ttggaaccag 961 accctgacaa aaggccggat atttaccagg tgtcctactt ctcatttaag ctactcaaga 1021 aagagtgccc aattccaaat gtacagaact ctcccattcc tgcaaagctt cctgaaccag 1081 tgaaagccag tgaggcagct gcaaaaaaga cccagccaaa ggccagactg acagatccca 1141 ttcccaccac agagacttca attgcacccc gccagaggcc taaagctggg cagactcagc 1201 cgaacccagg aatccttccc atccagccag cgctgacacc ccggaagagg gccactgttc 1261 agcccccacc tcaggctgca ggatccagca atcagcctgg ccttttagcc agtgttcccc 1321 aaccaaaacc ccaagcccca cccagccagc ctctgccgca aactcaggcc aagcagccac 1381 aggctcctcc cactccacag cagacgcctt ctactcaggc ccagggtctg cccgctcagg 1441 cccaggccac accccagcac cagcagcaac tcttcctcaa gcagcaacag cagcagcaac 1501 agccaccgcc agcacagcag cagccggcag gcacgtttta ccagcagcag caggcccaga 1561 ctcagcagtt tcaggcagta catccagcaa cccagcaacc agcaattgct cagttccctg 1621 tggtgtccca aggaggctct caacagcagc taatgcagaa tttctaccag cagcagcagc 1681 agcagcaaca acaacagcaa cagcaacagc tggccacagc cctgcatcaa caacagctga 1741 tgactcagca ggctgccttg cagcaaaagc ccactatggc agcaggacag cagccccagc 1801 cacagccagc tgcagcccca cagccagccc ctgcccagga accagcgatt caagccccag 1861 taagacaaca gccaaaggtt cagacaaccc cacctcctgc cgtccagggg cagaaagttg 1921 gatctctcac tccaccctca tcccccaaaa cccaacgtgc tgggcacagg cgtattctca 1981 gtgacgtaac ccacagtgca gtctttgggg tccctgccag caaatcaacc cagctgctcc 2041 aggcagctgc agctgaggcc agtctcaata agtccaagtc tgcaaccacc actccatcag 2101 gctctcctcg gacctctcaa caaaacgttt ataatccttc agaagggtct acgtggaatc 2161 cctttgatga cgataatttc tccaaactca cagctgaaga actgctaaac aaggactttg 2221 ccaagcttgg ggaaggcaaa catcccgaga agcttggagg ctcagctgag agtttgatcc 2281 caggctttca atcaacccaa ggtgatgctt ttgctacgac ctcattttct gctggaactg 2341 ctgaaaaaag gaagggtggg cagactgtgg actctggcct cccgcttcta agcgtgtctg 2401 atcctttcat tcctcttcaa gtacctgatg caccagaaaa actaattgag ggactcaaat 2461 ctcctgacac ttctcttctg ctccctgacc tcttgcctat gacagatcct tttggtagca 2521 cttctgatgc tgtaattgaa aaagctgatg ttgctgttga gagtctcata ccaggactgg 2581 agcccccagt tccccagcgc cTCCCATCTC AGACGGAATC TGTGACCTCG AATCGCACAG 2641 ATTCTCTCAC CGGGGAAGAT TCCCTGCTTG ATTGCTCTCT GCTCTCTAAC CCTACTACTG 2701 ACCTTCTGGA AGAGTTTGCC CCCACAGCAA TCTCTGCTCC AGTCCATAAA GCTGCAGAAG 2761 ATAGTAATCT CATCTCAGGT TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCTGAAGATG 2821 AGTTTGACCC TATTCCTGTA TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA 2881 GCAGTGGGAG CTCTGAGTCC AGTCTTCCCA ACCTAGCCAG GTCTTTACTG CTGGTGGATC 2941 AGCTCATAGA CCTGGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 3001 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 3061 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGTGAGATCG GGAATAACTT 3121 GTGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt