Construct: ORF TRCN0000491312
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014598.1_s317c1
- Derived from:
- ccsbBroadEn_09188
- DNA Barcode:
- CGAGTGTGCGGCGCCGCGCAGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB11FIP4 (84440)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491312
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84440 | RAB11FIP4 | RAB11 family interacting pr... | NM_032932.6 | 99.9% | 100% | 1770G>A |
| 2 | human | 84440 | RAB11FIP4 | RAB11 family interacting pr... | NM_001303542.3 | 83.1% | 82.7% | (many diffs) |
| 3 | human | 84440 | RAB11FIP4 | RAB11 family interacting pr... | NM_001346748.2 | 77.8% | 77.8% | 0_1ins423;1347G>A |
| 4 | human | 84440 | RAB11FIP4 | RAB11 family interacting pr... | NM_001346747.2 | 72.9% | 72.3% | (many diffs) |
| 5 | human | 84440 | RAB11FIP4 | RAB11 family interacting pr... | NM_001346749.2 | 59.1% | 59.1% | 0_1ins780;990G>A |
| 6 | mouse | 268451 | Rab11fip4 | RAB11 family interacting pr... | NM_175543.3 | 87.6% | 92.6% | (many diffs) |
| 7 | mouse | 268451 | Rab11fip4 | RAB11 family interacting pr... | XM_006533403.1 | 84.7% | 89.4% | (many diffs) |
| 8 | mouse | 268451 | Rab11fip4 | RAB11 family interacting pr... | XM_006533404.3 | 72.6% | 76.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1980
- ORF length:
- 1911
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgggcggc gcgggctggt cgggcgcccc cgcggctctg ctgcgctccg 121 tgcgccgcct gcgcgaggtg ttcgaggtgt gcggccgcga ccccgacggc ttcctgcgcg 181 tggagcgcgt cgcggcgctc ggactgcgct tcggccaggg cgaggaggtg gaaaaacttg 241 tgaaatattt ggatcccaac gacctgggga gaatcaactt caaggacttt tgccgggggg 301 tgttcgccat gaaagggtgc gaggagctgc tgaaggatgt gctgtcggtg gagagcgcgg 361 ggacgctgcc gtgcgcgcca gagatcccag actgcgtgga gcagggcagc gaggtcacag 421 gccccacctt tgctgatggc gagctcatcc ccagggaacc cggctttttt cccgaggacg 481 aggaggaggc tatgacgctg gcgccacctg agggccccca ggagttgtac acagacagcc 541 ccatggagag cactcagagc ctggaggggt ctgtcgggag tcctgccgag aaggacgggg 601 gacttggggg cctgtttctg ccagaagaca agtccctggt ccacactcca tccatgacga 661 cctcagacct ttctacacac tccaccacct cgctcatcag caatgaggag cagtttgaag 721 actatgggga gggtgacgat gtggactgtg cccccagcag cccttgcccc gatgatgaga 781 ccaggaccaa cgtctactcg gacctggggt cttcggtgtc ttccagtgcg gggcagacgc 841 ctaggaaaat gcggcacgtg tacaacagcg aattgctaga tgtttactgc tctcaatgct 901 gcaagaaaat caacctgctc aatgacttgg aagcccgact gaaaaacctg aaggccaaca 961 gccccaaccg aaagatctcc agcacggcct ttggacggca gctcatgcac agcagcaact 1021 tcagcagcag caatggcagc accgaagacc tgttccggga cagcattgac tcttgcgaca 1081 atgacatcac agagaaggta agcttcctgg aaaagaaggt gacagagctg gagaatgaca 1141 gcctgaccaa tggggacctg aagagcaagc tgaagcaaga gaacacacag ctggtgcaca 1201 gggtgcatga gctggaggag atggtgaagg atcaggagac cacggccgag caggctctgg 1261 aggaggaggc gcggcgccac cgcgaggcct acggcaagct ggagagggag aaggctaccg 1321 aggtggagct gctcaatgcc agggtgcagc agttggagga agaaaataca gagcttagaa 1381 caacagtgac tcggctcaag tctcaaacag agaaactgga tgaggagcgg cagcgcatgt 1441 ctgaccgtct ggaggacacc agcctgcggc tcaaagatga gatggacctg tacaagcgca 1501 tgatggacaa gctgcgacag aaccgccttg agttccagaa ggagcgggag gcgacgcagg 1561 agctcatcga ggacttgcgg aaggagctgg agcacctgca gatgtacaag ctggactgcg 1621 agcggccagg caggggccgc agtgcctcct ctggcctagg cgagttcaat gccagggccc 1681 gcgaggtgga gctcgagcac gaggtcaagc ggctcaagca ggagaattat aagctgcggg 1741 atcagaacga cgacttgaat gggcagattt tgagccTCAG CCTCTACGAA GCAAAAAACC 1801 TCTTTGCTGC CCAGACTAAA GCCCAGTCTC TGGCTGCAGA GATAGACACC GCCTCGCGCG 1861 ATGAGCTAAT GGAAGCCCTG AAGGAGCAGG AGGAGATCAA CTTCCGGCTG AGGCAGTACA 1921 TGGACAAGAT TATCCTCGCC ATCCTGGACC ACAATCCCTC CATCCTCGAG ATCAAACACT 1981 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2041 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2101 TTTATATATC TTGTGGAAAG GACGACGAGT GTGCGGCGCC GCGCAGCCAA CGCGTTAAGT 2161 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt